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  1. Mar 10, 2021
  2. Mar 02, 2021
  3. Jan 07, 2021
  4. Dec 10, 2020
    • van den Berg's avatar
      Use a tmp folder for MultiQC · b1baa6de
      van den Berg authored
      MultiQC can run out of /tmp space on execution nodes on shark, leading
      to incorrect results or crashes. This commit fixes this problem by
      specifying a default location for the python tempfiles that MultiQC uses
      via the environment variable TMPFILE inside of the shell block for the
      MultiQC rule.
      b1baa6de
  5. Aug 28, 2020
  6. Aug 27, 2020
  7. Aug 25, 2020
  8. Aug 24, 2020
  9. Aug 21, 2020
  10. Aug 20, 2020
  11. Aug 12, 2020
    • van den Berg's avatar
      Remove directory output from fastqc · 89da42b6
      van den Berg authored
      The directory output for the fastqc tasks is causing issues on the
      shared file system of the cluster, since it cannot properly determine
      the age of the folder. As a result, it re-runs the fastqc tasks every
      time a workflow is restarted, regardless of whether the task has already
      completed.
      
      To prevent this, a single dummy output file '.done' has been added to
      the fastqc tasks which will be written when fastqc exits successfully.
      89da42b6
  12. Aug 07, 2020
    • van den Berg's avatar
      Add an option to restrict BaseRecalibration · 095305f0
      van den Berg authored
      The base recalibration step of the pipeline can take up to 7 hours for
      WGS samples, which is a significant part of the total run time.
      
      The developers of GATK state that BQSR requires at least 100M bases per
      read group: "We usually expect to see more than 100M bases per read
      group; as a rule of thumb, larger numbers will work better."
      
      A human WGS sample with an average read depth of 43x has almost 1300
      times that amount of bases. The analysis of these samples would be sped
      up greatly by restricting BQSR to a single chromosome.
      095305f0
    • van den Berg's avatar
      Speed up cutadapt · b585ff80
      van den Berg authored
      Use 8 cores instead of just 1, according to the documentation speed
      should almost scale linear with the cores provided.
      
      Also reduce the compression level on the output file, since most time
      for cutadapt is spent re-compressing the data after trimming the
      adapters.
      b585ff80
  13. Jul 29, 2020
  14. Jul 28, 2020
  15. Jul 24, 2020
  16. Jul 22, 2020
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