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Commit 5e274379 authored by Peter van 't Hof's avatar Peter van 't Hof
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Update some content

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...@@ -89,6 +89,7 @@ At this moment the following variant callers can be used ...@@ -89,6 +89,7 @@ At this moment the following variant callers can be used
* <a href="https://www.broadinstitute.org/gatk/gatkdocs/org_broadinstitute_gatk_tools_walkers_genotyper_UnifiedGenotyper.php">unifiedgenotyper_allele</a> * <a href="https://www.broadinstitute.org/gatk/gatkdocs/org_broadinstitute_gatk_tools_walkers_genotyper_UnifiedGenotyper.php">unifiedgenotyper_allele</a>
* Only genotype a given list of alleles with UnifiedGenotyper * Only genotype a given list of alleles with UnifiedGenotyper
* <a href="https://samtools.github.io/bcftools/bcftools.html">bcftools</a> * <a href="https://samtools.github.io/bcftools/bcftools.html">bcftools</a>
* <a href="https://samtools.github.io/bcftools/bcftools.html">bcftools_singlesample</a>
* <a href="https://github.com/ekg/freebayes">freebayes</a> * <a href="https://github.com/ekg/freebayes">freebayes</a>
* [raw](../tools/MpileupToVcf) * [raw](../tools/MpileupToVcf)
...@@ -108,6 +109,8 @@ To view all possible config options please navigate to our Gitlab wiki page ...@@ -108,6 +109,8 @@ To view all possible config options please navigate to our Gitlab wiki page
| Namespace | Name | Type | Default | Function | | Namespace | Name | Type | Default | Function |
| ----------- | ---- | ----- | ------- | -------- | | ----------- | ---- | ----- | ------- | -------- |
| shiva | species | String | unknown_species | Name of species, like H.sapiens |
| shiva | reference_name | String | unknown_reference_name | Name of reference, like hg19 |
| shiva | reference_fasta | String | | reference to align to | | shiva | reference_fasta | String | | reference to align to |
| shiva | dbsnp | String | | vcf file of dbsnp records | | shiva | dbsnp | String | | vcf file of dbsnp records |
| shiva | variantcallers | List[String] | | variantcaller to use, see list | | shiva | variantcallers | List[String] | | variantcaller to use, see list |
...@@ -122,7 +125,6 @@ To view all possible config options please navigate to our Gitlab wiki page ...@@ -122,7 +125,6 @@ To view all possible config options please navigate to our Gitlab wiki page
| vcffilter | min_alternate_depth | Integer | 2 | Filter variants with at least x depth on the alternate allele | | vcffilter | min_alternate_depth | Integer | 2 | Filter variants with at least x depth on the alternate allele |
| vcffilter | min_samples_pass | Integer | 1 | Minimum amount of samples which pass custom filter (requires additional flags) | | vcffilter | min_samples_pass | Integer | 1 | Minimum amount of samples which pass custom filter (requires additional flags) |
| vcffilter | filter_ref_calls | Boolean | true | Remove reference calls | | vcffilter | filter_ref_calls | Boolean | true | Remove reference calls |
| vcfstats | reference | String | Path to reference to be used by `vcfstats` |
Since Shiva uses the [Mapping](mapping.md) pipeline internally, mapping config values can be specified as well. Since Shiva uses the [Mapping](mapping.md) pipeline internally, mapping config values can be specified as well.
For all the options, please see the corresponding documentation for the mapping pipeline. For all the options, please see the corresponding documentation for the mapping pipeline.
...@@ -151,26 +153,23 @@ The config for these therefore is: ...@@ -151,26 +153,23 @@ The config for these therefore is:
**Config example** **Config example**
```json ``` yaml
{ samples:
"samples": { SampleID:
"SampleID": { libraries:
"libraries": { lib_id_1:
"lib_id_1": { "bam": "YourBam.bam" }, bam: YourBam.bam
"lib_id_2": { "R1": "file_R1.fq.gz", "R2": "file_R2.fq.gz" } lib_id_2:
} R1: file_R1.fq.gz
} R2: file_R2.fq.gz
}, dbsnp: <dbsnp.vcf.gz>
"shiva": { vcffilter:
"reference": "<location of fasta of reference>", min_alternate_depth: 1
"variantcallers": [ "haplotypecaller", "unifiedgenotyper" ], output_dir: <output directory>
"dbsnp": "</path/to/dbsnp.vcf>", variantcallers:
"vcffilter": { - haplotypecaller
"min_alternate_depth": 1 - unifiedgenotyper
} - haplotypecaller_gvcf
},
"output_dir": "<output directory>"
}
``` ```
## References ## References
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