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biopet.biopet
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5e274379
Commit
5e274379
authored
9 years ago
by
Peter van 't Hof
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docs/pipelines/shiva.md
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View file @
5e274379
...
...
@@ -89,6 +89,7 @@ At this moment the following variant callers can be used
*
<a
href=
"https://www.broadinstitute.org/gatk/gatkdocs/org_broadinstitute_gatk_tools_walkers_genotyper_UnifiedGenotyper.php"
>
unifiedgenotyper_allele
</a>
*
Only genotype a given list of alleles with UnifiedGenotyper
*
<a
href=
"https://samtools.github.io/bcftools/bcftools.html"
>
bcftools
</a>
*
<a
href=
"https://samtools.github.io/bcftools/bcftools.html"
>
bcftools_singlesample
</a>
*
<a
href=
"https://github.com/ekg/freebayes"
>
freebayes
</a>
*
[
raw
](
../tools/MpileupToVcf
)
...
...
@@ -108,6 +109,8 @@ To view all possible config options please navigate to our Gitlab wiki page
| Namespace | Name | Type | Default | Function |
| ----------- | ---- | ----- | ------- | -------- |
| shiva | species | String | unknown_species | Name of species, like H.sapiens |
| shiva | reference_name | String | unknown_reference_name | Name of reference, like hg19 |
| shiva | reference_fasta | String | | reference to align to |
| shiva | dbsnp | String | | vcf file of dbsnp records |
| shiva | variantcallers | List[String] | | variantcaller to use, see list |
...
...
@@ -122,7 +125,6 @@ To view all possible config options please navigate to our Gitlab wiki page
| vcffilter | min_alternate_depth | Integer | 2 | Filter variants with at least x depth on the alternate allele |
| vcffilter | min_samples_pass | Integer | 1 | Minimum amount of samples which pass custom filter (requires additional flags) |
| vcffilter | filter_ref_calls | Boolean | true | Remove reference calls |
| vcfstats | reference | String | Path to reference to be used by
`vcfstats`
|
Since Shiva uses the
[
Mapping
](
mapping.md
)
pipeline internally, mapping config values can be specified as well.
For all the options, please see the corresponding documentation for the mapping pipeline.
...
...
@@ -151,26 +153,23 @@ The config for these therefore is:
**Config example**
```
json
{
"samples"
:
{
"SampleID"
:
{
"libraries"
:
{
"lib_id_1"
:
{
"bam"
:
"YourBam.bam"
},
"lib_id_2"
:
{
"R1"
:
"file_R1.fq.gz"
,
"R2"
:
"file_R2.fq.gz"
}
}
}
},
"shiva"
:
{
"reference"
:
"<location of fasta of reference>"
,
"variantcallers"
:
[
"haplotypecaller"
,
"unifiedgenotyper"
],
"dbsnp"
:
"</path/to/dbsnp.vcf>"
,
"vcffilter"
:
{
"min_alternate_depth"
:
1
}
},
"output_dir"
:
"<output directory>"
}
```
yaml
samples
:
SampleID
:
libraries
:
lib_id_1
:
bam
:
YourBam.bam
lib_id_2
:
R1
:
file_R1.fq.gz
R2
:
file_R2.fq.gz
dbsnp
:
<dbsnp.vcf.gz>
vcffilter
:
min_alternate_depth
:
1
output_dir
:
<output directory>
variantcallers
:
-
haplotypecaller
-
unifiedgenotyper
-
haplotypecaller_gvcf
```
## References
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