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NGS-intro-course
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a56431c1
Commit
a56431c1
authored
10 years ago
by
Michiel van Galen
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Spellchecked practical one
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a56431c1
...
...
@@ -76,7 +76,7 @@ Now, create a directory and a text file using \texttt{nano}.
$
nano file
1
.txt
\end
{
lstlisting
}
We have now opened the
\texttt
{
nano
}
text editor and are editin
t
the content
We have now opened the
\texttt
{
nano
}
text editor and are editin
g
the content
of the file
\texttt
{
file
1
.txt
}
. Try to create a
\texttt
{
FASTA
}
formatted file,
something like:
...
...
@@ -251,7 +251,7 @@ checked for variants and annotated.
\subsection
{
Get the input
}
First we must fetch the course data for the practicals. We have precompiled a
small human test set of short reads in an online repository. Use the next
command to pull eveything from that repository for you to work with.
command to pull eve
r
ything from that repository for you to work with.
\medskip
How this exactly works is not important for now but if things work as
...
...
@@ -269,14 +269,14 @@ providing everything you need to continue.
\medskip
% Quality control
\subsection
{
Quality control with Fast
qc
}
Every tool which is systemwide available can simply be run by typing the name
of the tool. Try typing part of the toolname and hit TAB twice. It will
automatically autocomplete the name or give suggestions if it's not sure yet.
\subsection
{
Quality control with Fast
QC
}
Every tool which is system
wide available can simply be run by typing the name
of the tool. Try typing part of the tool
name and hit TAB twice. It will
automatically auto
-
complete the name or give suggestions if it's not sure yet.
\medskip
Most tools will show some documentation if you start them without any options.
If this doesn't happe
d
you have to usually add the
-
h which stands for help.
If this doesn't happe
n
you have to usually add the
-
h which stands for help.
\medskip
\begin
{
itemize
}
...
...
@@ -337,7 +337,7 @@ name for the output as last option.
\medskip
For downstream purpose we want one more option, namely the output to be in SAM
format. We achive this by adding the -S parameter. Note these optional
format. We achi
e
ve this by adding the -S parameter. Note these optional
arguments come first before the others. (As you can see in the help)
\medskip
...
...
@@ -360,7 +360,7 @@ less disk space! Let's convert the file.
The first option we need to give Samtools is which function to use, in our
case we use ``view''. After that we give ``
-
Sb'', basically telling Samtools that
our input is SAM and we would like BAM output. Finally we redirect the output
to a new outputfile using the greater than sign.
to a new output
file using the greater than sign.
\medskip
For many purposes it is convenient, and often even required, to have your BAM
...
...
@@ -370,7 +370,7 @@ useful. To sort the file, we simply call Samtools sort. The first argument is
the input file, followed by the output file name.
\medskip
Note that we omit the .bam extension for the output filename. This will be done
Note that we omit the .bam extension for the output file
name. This will be done
by Samtools.
\medskip
...
...
@@ -405,7 +405,7 @@ As explained, the mpileup data will be fed directly into bcftools. At this
point bcftools will go over the pileup format, picks out the variants and
describes them into a bcf file.
(
A binary format of the vcf format.
)
In the next
step we will convert the bcf file to a readable vcf file while at the same time
we apply a filterstep from vcfutils.
we apply a filter
step from vcfutils.
\medskip
\begin
{
itemize
}
...
...
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