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Commit 9378cc17 authored by Michiel van Galen's avatar Michiel van Galen
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Spellcheck practical 2

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......@@ -18,7 +18,7 @@
}
\title{NGS Introduction Course\\
{\Large Practical two, Piplines}}
{\Large Practical two, Pipelines}}
\date{Friday, 4 April 2014}
\author{Jeroen Laros, Michiel van Galen}
......@@ -36,10 +36,10 @@ Log in to the virtual machine as described in the first practical. Finish these
exercises first then continue with this one.
\bigskip
Anlysis pipelines can vary from a couple of very simple lines of code, to
Analysis pipelines can vary from a couple of very simple lines of code, to
complex frameworks working on multiple computing nodes. However, the idea
behind it is always the same. Make your analysis easier, while at the same time
it becomes reproducable, documented and easier to share.
it becomes reproducible, documented and easier to share.
\medskip
Today we will use bash to create a basic pipeline. Unknowingly, you have
......@@ -59,12 +59,12 @@ specify the interpreter. In Linux we us chmod for this.
\medskip
One last additional piece of information your system is missing, is which
interpertaor it should use. We store this in the very first line of the script
interpreter it should use. We store this in the very first line of the script
and is written like this ``\#!/bin/bash''.
\medskip
This is all you need to know to write your first pipeline. Bash offers a lot
more possibilities than just using Linux powertools, navigation and starting
more possibilities than just using Linux power tools, navigation and starting
other software. For example, to make the pipeline easier to re-use with other
data, we can give a bash script parameters. Just like Bowtie, where you can
supply your fastq file. This is done by using the reserved variable \$1 inside
......@@ -99,7 +99,7 @@ a script that combines the tools from the first practical into a pipeline:
\medskip
\begin{itemize}
\item Inlcude these steps:
\item Include these steps:
\begin{itemize}
\item Align a fastq file to the mtDNA
\item Convert the SAM to a sorted BAM
......
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