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Commit 7079cd57 authored by Michiel van Galen's avatar Michiel van Galen
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Spellchecked and updated getting started

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../presentation-pics/pics/annotation.png
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......@@ -118,7 +118,7 @@
\begin{lstlisting}[language=none, caption={Copy and move}]
$ ls
original.txt work
$ cp orginal.txt another.txt
$ cp original.txt another.txt
$ ls
another.txt original.txt work
$ mv original.txt archive.txt
......@@ -291,7 +291,7 @@
\begin{pframe}
\begin{itemize}
\item Many times it's helpful to do something for each something
\item A simple for-oneliner in bash can help us out
\item A simple for-one-liner in bash can help us out
\item for this in that; do something; done
\end{itemize}
\begin{lstlisting}[language=none, caption={For loop}]
......@@ -389,7 +389,7 @@
\section{Working with NGS tools}
\subsection{Analysis workflow}
\begin{pframe}
For this course we will go over the steps to analyse a human dataset short reads for variants:
For this course we will go over the steps to analyze a human dataset short reads for variants:
\smallskip
\begin{itemize}
\item Quality control
......@@ -408,7 +408,7 @@
\item Assess the quality of your data in a fastq file
\end{itemize}
\begin{figure}
\caption{Fastqc}
\caption{FastQC}
\centering
\includegraphics[width=0.5\textwidth]{pretrimmed_qscores}
\end{figure}
......@@ -418,7 +418,7 @@
\begin{pframe}
\begin{itemize}
\item Sickle: A windowed adaptive trimming tool for FASTQ files using quality.
\item Only maintain the high-quality bases in a given windowsize
\item Only maintain the high-quality bases in a given window size
\item Works on single and paired end
\end{itemize}
\begin{lstlisting}[language=none, caption={}]
......@@ -432,6 +432,18 @@
\end{lstlisting}
\end{pframe}
\subsection{Alignment}
\begin{pframe}
High troughput mapping of reads to a known reference genome
\begin{itemize}
\item Allow mismatches
\end{itemize}
\begin{figure}
\centering
\includegraphics[width=0.68\textwidth]{varcall}
\end{figure}
\end{pframe}
\subsection{Alignment}
\begin{pframe}
\begin{itemize}
......@@ -464,6 +476,15 @@
\end{itemize}
\end{pframe}
\subsection{Annotation}
\begin{pframe}
Adding some context to your variants greatly benefits the interpretation
\begin{figure}
\centering
\includegraphics[width=1\textwidth]{annotation}
\end{figure}
\end{pframe}
\subsection{Annotation}
\begin{pframe}
\begin{itemize}
......@@ -487,8 +508,8 @@
\item You have gotten an idea of a small selection of NGS tools
\bigskip
\item In the practical you will learn how to use these tools
\item This knowledge can be applied to install and use your own tools of choice
\item In the practical you will learn how to use and combine these tools
\item This knowledge can be applied to install and use the tools of your choice
\end{itemize}
\end{pframe}
......@@ -496,7 +517,7 @@
\lastpagetemplate
\begin{pframe}
\begin{center}
Acknowledgements:
Acknowledgments:
\bigskip
\bigskip
......
../presentation-pics/pics/varcall.gif
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