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courses
NGS-intro-course
Commits
7079cd57
Commit
7079cd57
authored
10 years ago
by
Michiel van Galen
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Spellchecked and updated getting started
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getting_started/annotation.png
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getting_started/annotation.png
getting_started/getting_started.tex
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getting_started/getting_started.tex
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getting_started/getting_started.tex
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@@ -118,7 +118,7 @@
\begin
{
lstlisting
}
[
language
=
none, caption
=
{
Copy and move
}
]
$
ls
original.txt work
$
cp orginal.txt another.txt
$
cp or
i
ginal.txt another.txt
$
ls
another.txt original.txt work
$
mv original.txt archive.txt
...
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@@ -291,7 +291,7 @@
\begin{pframe}
\begin{itemize}
\item
Many times it's helpful to do something for each something
\item
A simple for-oneliner in bash can help us out
\item
A simple for-one
-
liner in bash can help us out
\item
for this in that; do something; done
\end{itemize}
\begin{lstlisting}
[language=none, caption=
{
For loop
}
]
...
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@@ -389,7 +389,7 @@
\section
{
Working with NGS tools
}
\subsection
{
Analysis workflow
}
\begin
{
pframe
}
For this course we will go over the steps to analy
s
e a human dataset short reads for variants:
For this course we will go over the steps to analy
z
e a human dataset short reads for variants:
\smallskip
\begin
{
itemize
}
\item
Quality control
...
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@@ -408,7 +408,7 @@
\item
Assess the quality of your data in a fastq file
\end
{
itemize
}
\begin
{
figure
}
\caption
{
Fast
qc
}
\caption
{
Fast
QC
}
\centering
\includegraphics
[
width
=
0
.
5
\textwidth
]
{
pretrimmed
_
qscores
}
\end
{
figure
}
...
...
@@ -418,7 +418,7 @@
\begin
{
pframe
}
\begin
{
itemize
}
\item
Sickle: A windowed adaptive trimming tool for FASTQ files using quality.
\item
Only maintain the high
-
quality bases in a given windowsize
\item
Only maintain the high
-
quality bases in a given window
size
\item
Works on single and paired end
\end
{
itemize
}
\begin
{
lstlisting
}
[
language
=
none, caption
=
{}
]
...
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@@ -432,6 +432,18 @@
\end
{
lstlisting
}
\end
{
pframe
}
\subsection
{
Alignment
}
\begin
{
pframe
}
High troughput mapping of reads to a known reference genome
\begin
{
itemize
}
\item
Allow mismatches
\end
{
itemize
}
\begin
{
figure
}
\centering
\includegraphics
[
width
=
0
.
68
\textwidth
]
{
varcall
}
\end
{
figure
}
\end
{
pframe
}
\subsection
{
Alignment
}
\begin
{
pframe
}
\begin
{
itemize
}
...
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@@ -464,6 +476,15 @@
\end
{
itemize
}
\end
{
pframe
}
\subsection
{
Annotation
}
\begin
{
pframe
}
Adding some context to your variants greatly benefits the interpretation
\begin
{
figure
}
\centering
\includegraphics
[
width
=
1
\textwidth
]
{
annotation
}
\end
{
figure
}
\end
{
pframe
}
\subsection
{
Annotation
}
\begin
{
pframe
}
\begin
{
itemize
}
...
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@@ -487,8 +508,8 @@
\item
You have gotten an idea of a small selection of NGS tools
\bigskip
\item
In the practical you will learn how to use these tools
\item
This knowledge can be applied to install and use
your own
tools of choice
\item
In the practical you will learn how to use
and combine
these tools
\item
This knowledge can be applied to install and use
the
tools of
your
choice
\end
{
itemize
}
\end
{
pframe
}
...
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@@ -496,7 +517,7 @@
\lastpagetemplate
\begin
{
pframe
}
\begin
{
center
}
Acknowledg
e
ments:
Acknowledgments:
\bigskip
\bigskip
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