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biowdl
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!92
Dockerize GATK. Scattering now works with containers. Tabix another docker image.
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Dockerize GATK. Scattering now works with containers. Tabix another docker image.
BIOWDL-199
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develop
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Ruben Vorderman
requested to merge
BIOWDL-199
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develop
6 years ago
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82cc7609
Merge branch 'develop' into BIOWDL-199
· 82cc7609
Ruben Vorderman
authored
6 years ago
hisat2.wdl
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version 1.0
task Hisat2 {
input {
File indexDirectory
String indexBasename
File inputR1
File? inputR2
String outputBam
String sample
String library
String readgroup
String platform = "illumina"
Boolean downstreamTranscriptomeAssembly = true
Int threads = 1
Int memory = 48
String dockerTag = "2388ff67fc407dad75774291ca5038f40cac4be0-0"
}
command {
set -e -o pipefail
mkdir -p $(dirname ~{outputBam})
hisat2 \
-p ~{threads} \
-x ~{indexDirectory}/~{indexBasename} \
~{true="-1" false="-U" defined(inputR2)} ~{inputR1} \
~{"-2" + inputR2} \
--rg-id ~{readgroup} \
--rg 'SM:~{sample}' \
--rg 'LB:~{library}' \
--rg 'PL:~{platform}' \
~{true="--dta" false="" downstreamTranscriptomeAssembly} \
| samtools sort > ~{outputBam}
}
output {
File bamFile = outputBam
}
runtime {
memory: (memory / threads) + 1
cpu: threads + 1
docker: "quay.io/biocontainers/mulled-v2-a97e90b3b802d1da3d6958e0867610c718cb5eb1:" + dockerTag
}
}
\ No newline at end of file
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