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biopet.biopet
Commits
ceaa8fd0
Commit
ceaa8fd0
authored
9 years ago
by
Wai Yi Leung
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Handle RNA seq files, sam filter only takes the primary alignment
parent
b4a13e74
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public/gears/src/main/scala/nl/lumc/sasc/biopet/pipelines/gears/Gears.scala
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...ain/scala/nl/lumc/sasc/biopet/pipelines/gears/Gears.scala
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public/gears/src/main/scala/nl/lumc/sasc/biopet/pipelines/gears/Gears.scala
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ceaa8fd0
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@@ -69,7 +69,7 @@ class Gears(val root: Configurable) extends QScript with SummaryQScript {
// sambamba view -f bam -F "unmapped or mate_is_unmapped" <alnFile> > <extracted.bam>
val
samFilterUnmapped
=
new
SambambaView
(
qscript
)
samFilterUnmapped
.
input
=
bamfile
samFilterUnmapped
.
filter
=
Some
(
"unmapped or mate_is_unmapped"
)
samFilterUnmapped
.
filter
=
Some
(
"
(
unmapped or mate_is_unmapped
) and not (secondary_alignment)
"
)
samFilterUnmapped
.
output
=
new
File
(
outputDir
,
s
"$outputName.unmapped.bam"
)
samFilterUnmapped
.
isIntermediate
=
false
add
(
samFilterUnmapped
)
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