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biopet.biopet
Commits
a68e86c1
Commit
a68e86c1
authored
Oct 27, 2015
by
Wai Yi Leung
Browse files
Refactor usage of Delly pipeline a bit by adding CatVariants and moving Delly to Shiva
parent
fe9f50f7
Changes
4
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Side-by-side
public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/extensions/gatk/CatVariants.scala
0 → 100644
View file @
a68e86c1
/**
* Biopet is built on top of GATK Queue for building bioinformatic
* pipelines. It is mainly intended to support LUMC SHARK cluster which is running
* SGE. But other types of HPC that are supported by GATK Queue (such as PBS)
* should also be able to execute Biopet tools and pipelines.
*
* Copyright 2014 Sequencing Analysis Support Core - Leiden University Medical Center
*
* Contact us at: sasc@lumc.nl
*
* A dual licensing mode is applied. The source code within this project that are
* not part of GATK Queue is freely available for non-commercial use under an AGPL
* license; For commercial users or users who do not want to follow the AGPL
* license, please contact us to obtain a separate license.
*/
package
nl.lumc.sasc.biopet.extensions.gatk
import
java.io.File
import
nl.lumc.sasc.biopet.utils.config.Configurable
import
org.broadinstitute.gatk.utils.commandline.
{
Input
,
Output
}
class
CatVariants
(
val
root
:
Configurable
)
extends
Gatk
{
val
analysisType
=
"CatVariants"
@Input
(
doc
=
""
,
required
=
true
)
var
inputFiles
:
List
[
File
]
=
Nil
@Output
(
doc
=
""
,
required
=
true
)
var
outputFile
:
File
=
null
override
def
cmdLine
=
super
.
cmdLine
+
(
for
(
file
<-
inputFiles
)
yield
{
required
(
"-V"
,
file
)
}).
mkString
+
required
(
"-o"
,
outputFile
)
}
object
CatVariants
{
def
apply
(
root
:
Configurable
,
input
:
List
[
File
],
output
:
File
)
:
CatVariants
=
{
val
cv
=
new
CatVariants
(
root
)
cv
.
inputFiles
=
input
cv
.
outputFile
=
output
cv
}
}
\ No newline at end of file
public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/
extensions/delly
/Delly.scala
→
public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/
pipelines/shiva
/Delly.scala
View file @
a68e86c1
...
...
@@ -13,17 +13,19 @@
* license; For commercial users or users who do not want to follow the AGPL
* license, please contact us to obtain a separate license.
*/
package
nl.lumc.sasc.biopet.
extensions.delly
package
nl.lumc.sasc.biopet.
pipelines.shiva
import
java.io.File
import
nl.lumc.sasc.biopet.core.
{
Reference
,
BiopetQScript
,
PipelineCommand
}
import
nl.lumc.sasc.biopet.
utils.config.Configurable
import
nl.lumc.sasc.biopet.core.
summary.SummaryQScript
import
nl.lumc.sasc.biopet.
core.
{
PipelineCommand
,
Reference
}
import
nl.lumc.sasc.biopet.extensions.Ln
import
nl.lumc.sasc.biopet.extensions.delly.DellyCaller
import
nl.lumc.sasc.biopet.extensions.gatk.CatVariants
import
nl.lumc.sasc.biopet.utils.config.Configurable
import
org.broadinstitute.gatk.queue.QScript
import
org.broadinstitute.gatk.queue.extensions.gatk.CatVariants
class
Delly
(
val
root
:
Configurable
)
extends
QScript
with
BiopetQScript
with
Reference
{
class
Delly
(
val
root
:
Configurable
)
extends
QScript
with
Reference
with
SummaryQScript
{
def
this
()
=
this
(
null
)
@Input
(
doc
=
"Input file (bam)"
)
...
...
@@ -47,9 +49,30 @@ class Delly(val root: Configurable) extends QScript with BiopetQScript with Refe
if
(
outputVcf
==
null
)
outputVcf
=
new
File
(
workDir
,
outputName
+
".delly.vcf"
)
}
override
def
summaryFile
:
File
=
new
File
(
outputDir
,
"Delly.summary.json"
)
override
def
summaryFiles
:
Map
[
String
,
File
]
=
Map
.
empty
++
Map
(
"input"
->
input
)
++
Map
(
"outputVCF"
->
outputVcf
)
override
def
summarySettings
=
{
Map
(
"input"
->
input
,
"workDir"
->
workDir
,
"outputVCF"
->
outputVcf
,
"outputName"
->
outputName
,
"del"
->
del
,
"dup"
->
dup
,
"inv"
->
inv
,
"tra"
->
tra
)
}
def
biopetScript
()
{
// write the pipeline here
logger
.
info
(
"Configuring Delly pipeline"
)
var
outputFiles
:
Map
[
String
,
File
]
=
Map
()
var
vcfFiles
:
Map
[
String
,
File
]
=
Map
()
...
...
@@ -89,15 +112,13 @@ class Delly(val root: Configurable) extends QScript with BiopetQScript with Refe
// we need to merge the vcf's
val
finalVCF
=
if
(
vcfFiles
.
size
>
1
)
{
// do merging
// CatVariants is a $org.broadinstitute.gatk.utils.commandline.CommandLineProgram$;
//TODO: convert to biopet extension
val
variants
=
new
CatVariants
()
variants
.
variant
=
vcfFiles
.
values
.
toList
val
variants
=
new
CatVariants
(
this
)
variants
.
inputFiles
=
vcfFiles
.
values
.
toList
variants
.
outputFile
=
this
.
outputVcf
variants
.
reference
=
referenceFasta
()
//
variants.reference = referenceFasta()
// add the job
//
add(variants)
Some
(
output
Vcf
)
add
(
variants
)
Some
(
variants
.
output
File
)
}
else
if
(
vcfFiles
.
size
==
1
)
{
// TODO: pretify this
val
ln
=
Ln
(
this
,
vcfFiles
.
head
.
_2
,
this
.
outputVcf
,
relative
=
true
)
...
...
@@ -110,8 +131,6 @@ class Delly(val root: Configurable) extends QScript with BiopetQScript with Refe
}
object
Delly
extends
PipelineCommand
{
override
val
pipeline
=
"/nl/lumc/sasc/biopet/extensions/svcallers/Delly/Delly.class"
def
apply
(
root
:
Configurable
,
input
:
File
,
workDir
:
File
)
:
Delly
=
{
val
dellyPipeline
=
new
Delly
(
root
)
dellyPipeline
.
input
=
input
...
...
public/shiva/src/main/scala/nl/lumc/sasc/biopet/pipelines/shiva/ShivaSvCalling.scala
View file @
a68e86c1
...
...
@@ -17,10 +17,9 @@ package nl.lumc.sasc.biopet.pipelines.shiva
import
htsjdk.samtools.SamReaderFactory
import
nl.lumc.sasc.biopet.core.summary.SummaryQScript
import
nl.lumc.sasc.biopet.core.
{
PipelineCommand
,
Reference
,
SampleLibraryTag
}
import
nl.lumc.sasc.biopet.core.
{
PipelineCommand
,
Reference
,
SampleLibraryTag
}
import
nl.lumc.sasc.biopet.extensions.breakdancer.Breakdancer
import
nl.lumc.sasc.biopet.extensions.clever.CleverCaller
import
nl.lumc.sasc.biopet.extensions.delly.Delly
import
nl.lumc.sasc.biopet.utils.Logging
import
nl.lumc.sasc.biopet.utils.config.Configurable
import
org.broadinstitute.gatk.queue.QScript
...
...
@@ -135,6 +134,7 @@ class ShivaSvCalling(val root: Configurable) extends QScript with SummaryQScript
val
delly
=
Delly
(
qscript
,
bamFile
,
dellyDir
)
delly
.
outputName
=
sample
addAll
(
delly
.
functions
)
addSummaryQScript
(
delly
)
}
}
}
...
...
public/yamsvp/src_old/main/scala/nl/lumc/sasc/biopet/pipelines/yamsvp/Yamsvp.scala
View file @
a68e86c1
...
...
@@ -21,15 +21,15 @@ package nl.lumc.sasc.biopet.pipelines.yamsvp
import
java.io.File
import
nl.lumc.sasc.biopet.utils.config.Configurable
import
nl.lumc.sasc.biopet.core.
{
MultiSampleQScript
,
PipelineCommand
}
import
nl.lumc.sasc.biopet.core.
{
MultiSampleQScript
,
PipelineCommand
}
import
nl.lumc.sasc.biopet.extensions.Ln
import
nl.lumc.sasc.biopet.extensions.breakdancer.Breakdancer
import
nl.lumc.sasc.biopet.extensions.clever.CleverCaller
import
nl.lumc.sasc.biopet.extensions.igvtools.IGVToolsCount
import
nl.lumc.sasc.biopet.extensions.sambamba.
{
SambambaMarkdup
,
SambambaMerge
}
import
nl.lumc.sasc.biopet.extensions.sambamba.
{
SambambaMarkdup
,
SambambaMerge
}
import
nl.lumc.sasc.biopet.pipelines.shiva.Delly
import
nl.lumc.sasc.biopet.utils.config.Configurable
//import nl.lumc.sasc.biopet.extensions.pindel.Pindel
import
nl.lumc.sasc.biopet.extensions.delly.Delly
import
nl.lumc.sasc.biopet.pipelines.bammetrics.BamMetrics
import
nl.lumc.sasc.biopet.pipelines.mapping.Mapping
import
org.broadinstitute.gatk.queue.QScript
...
...
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