Merge branch 'feature-yamsvp' into 'develop'

Feature yamsvp

Convert yamsvp to also work from a bam file and be included in Shiva

See merge request !201

public/basty/src/main/scala/nl/lumc/sasc/biopet/pipelines/basty/BastyTrait.scala

@@ -179,7 +179,7 @@ trait BastyTrait extends MultiSampleQScript {
                        snpsOnly: Boolean = false): FastaOutput = {
     val bastyGenerateFasta = new BastyGenerateFasta(this)
     bastyGenerateFasta.outputName = if (outputName != null) outputName else sampleName
-    bastyGenerateFasta.inputVcf = shiva.variantcalling.get.finalFile
+    bastyGenerateFasta.inputVcf = shiva.variantCalling.get.finalFile
     if (shiva.samples.contains(sampleName)) {
       bastyGenerateFasta.bamFile = shiva.samples(sampleName).preProcessBam.get
     }

public/biopet-framework/src/main/scala/nl/lumc/sasc/biopet/extensions/breakdancer/Breakdancer.scala

@@ -18,38 +18,35 @@ package nl.lumc.sasc.biopet.extensions.breakdancer
 import java.io.File
 
 import nl.lumc.sasc.biopet.core.config.Configurable
-import nl.lumc.sasc.biopet.core.{ BiopetQScript, PipelineCommand }
+import nl.lumc.sasc.biopet.core.{ Reference, BiopetQScript, PipelineCommand }
 import org.broadinstitute.gatk.queue.QScript
 
 /// Breakdancer is actually a mini pipeline executing binaries from the breakdancer package
-class Breakdancer(val root: Configurable) extends QScript with BiopetQScript {
+class Breakdancer(val root: Configurable) extends QScript with BiopetQScript with Reference {
   def this() = this(null)
 
   @Input(doc = "Input file (bam)")
   var input: File = _
 
-  @Input(doc = "Reference Fasta file")
-  var reference: File = _
-
   @Argument(doc = "Work directory")
-  var workdir: String = _
+  var workDir: File = _
 
   var deps: List[File] = Nil
 
   @Output(doc = "Breakdancer config")
   lazy val configfile: File = {
-    new File(workdir + "/" + input.getName.substring(0, input.getName.lastIndexOf(".bam")) + ".breakdancer.cfg")
+    new File(workDir, input.getName.substring(0, input.getName.lastIndexOf(".bam")) + ".breakdancer.cfg")
   }
   @Output(doc = "Breakdancer raw output")
   lazy val outputraw: File = {
-    new File(workdir + "/" + input.getName.substring(0, input.getName.lastIndexOf(".bam")) + ".breakdancer.tsv")
+    new File(workDir, input.getName.substring(0, input.getName.lastIndexOf(".bam")) + ".breakdancer.tsv")
   }
   @Output(doc = "Breakdancer VCF output")
   lazy val outputvcf: File = {
-    new File(workdir + "/" + input.getName.substring(0, input.getName.lastIndexOf(".bam")) + ".breakdancer.vcf")
+    new File(workDir, input.getName.substring(0, input.getName.lastIndexOf(".bam")) + ".breakdancer.vcf")
   }
 
-  override def init() {
+  override def init(): Unit = {
   }
 
   def biopetScript() {
@@ -72,11 +69,10 @@ class Breakdancer(val root: Configurable) extends QScript with BiopetQScript {
 }
 
 object Breakdancer extends PipelineCommand {
-  def apply(root: Configurable, input: File, reference: File, runDir: String): Breakdancer = {
+  def apply(root: Configurable, input: File, runDir: File): Breakdancer = {
     val breakdancer = new Breakdancer(root)
     breakdancer.input = input
-    breakdancer.reference = reference
-    breakdancer.workdir = runDir
+    breakdancer.workDir = runDir
     breakdancer.init()
     breakdancer.biopetScript()
     breakdancer

public/biopet-framework/src/main/scala/nl/lumc/sasc/biopet/extensions/breakdancer/BreakdancerCaller.scala

@@ -51,8 +51,8 @@ class BreakdancerCaller(val root: Configurable) extends BiopetCommandLineFunctio
   var x: Option[Int] = config("x", default = 1000)
   var b: Option[Int] = config("b", default = 100)
   var t: Boolean = config("t", default = false)
-  var d: String = config("d")
-  var g: String = config("g")
+  var d: Option[String] = config("d")
+  var g: Option[String] = config("g")
   var l: Boolean = config("l", default = false)
   var a: Boolean = config("a", default = false)
   var h: Boolean = config("h", default = false)

public/biopet-framework/src/main/scala/nl/lumc/sasc/biopet/extensions/clever/Clever.scala

-/**
- * Biopet is built on top of GATK Queue for building bioinformatic
- * pipelines. It is mainly intended to support LUMC SHARK cluster which is running
- * SGE. But other types of HPC that are supported by GATK Queue (such as PBS)
- * should also be able to execute Biopet tools and pipelines.
- *
- * Copyright 2014 Sequencing Analysis Support Core - Leiden University Medical Center
- *
- * Contact us at: sasc@lumc.nl
- *
- * A dual licensing mode is applied. The source code within this project that are
- * not part of GATK Queue is freely available for non-commercial use under an AGPL
- * license; For commercial users or users who do not want to follow the AGPL
- * license, please contact us to obtain a separate license.
- */
-package nl.lumc.sasc.biopet.extensions.clever
-
-import java.io.File
-
-import nl.lumc.sasc.biopet.core.config.Configurable
-import nl.lumc.sasc.biopet.core.{ BiopetQScript, PipelineCommand }
-import org.broadinstitute.gatk.queue.QScript
-
-class Clever(val root: Configurable) extends QScript with BiopetQScript {
-  def this() = this(null)
-
-  @Input(doc = "Input file (bam)")
-  var input: File = _
-
-  @Input(doc = "Reference")
-  var reference: File = _
-
-  @Argument(doc = "Work directory")
-  var workdir: String = _
-
-  @Argument(doc = "Current working directory")
-  var cwd: String = _
-
-  override def init() {
-  }
-
-  def biopetScript() {
-    // write the pipeline here
-    logger.info("Starting Clever Pipeline")
-
-    /// start clever and then copy the vcf into the root directory "<sample>.clever/"
-    val clever = CleverCaller(this, input, reference, cwd, workdir)
-    outputFiles += ("clever_vcf" -> clever.outputvcf)
-    add(clever)
-  }
-}
-
-object Clever extends PipelineCommand {
-  override val pipeline = "/nl/lumc/sasc/biopet/extensions/svcallers/Clever/Clever.class"
-
-  def apply(root: Configurable, input: File, runDir: String): Clever = {
-    val cleverpipeline = new Clever(root)
-    cleverpipeline.input = input
-    cleverpipeline.workdir = runDir
-    cleverpipeline.init()
-    cleverpipeline.biopetScript()
-    cleverpipeline
-  }
-
-}
\ No newline at end of file

public/biopet-framework/src/main/scala/nl/lumc/sasc/biopet/extensions/clever/CleverCaller.scala

@@ -2,18 +2,19 @@ package nl.lumc.sasc.biopet.extensions.clever
 
 import java.io.File
 
-import nl.lumc.sasc.biopet.core.BiopetCommandLineFunction
+import nl.lumc.sasc.biopet.core.{ Reference, BiopetCommandLineFunction }
 import nl.lumc.sasc.biopet.core.config.Configurable
 import org.broadinstitute.gatk.utils.commandline.{ Argument, Input, Output }
 
-class CleverCaller(val root: Configurable) extends BiopetCommandLineFunction {
+class CleverCaller(val root: Configurable) extends BiopetCommandLineFunction with Reference {
   executable = config("exe", default = "clever")
 
-  private lazy val versionexecutable: File = config("version_exe", default = new File(executable).getParent + "/ctk-version")
+  private lazy val versionExecutable: File = config("version_exe", default = new File(executable).getParent + "/ctk-version")
 
   override def defaultThreads = 8
+  override def defaultCoreMemory = 3.0
 
-  override def versionCommand = versionexecutable.getAbsolutePath
+  override def versionCommand = versionExecutable.getAbsolutePath
   override def versionRegex = """(.*)""".r
   override def versionExitcode = List(0, 1)
 
@@ -23,19 +24,17 @@ class CleverCaller(val root: Configurable) extends BiopetCommandLineFunction {
   @Input(doc = "Reference")
   var reference: File = _
 
-  @Argument(doc = "Work directory")
-  var workdir: String = _
-
-  var cwd: String = _
+  protected def workDir: File = new File(cwd, "work")
+  var cwd: File = _
 
   @Output(doc = "Clever VCF output")
   lazy val outputvcf: File = {
-    new File(cwd + "predictions.vcf")
+    new File(cwd, "predictions.vcf")
   }
 
   @Output(doc = "Clever raw output")
   lazy val outputraw: File = {
-    new File(workdir + "predictions.raw.txt")
+    new File(workDir, "predictions.raw.txt")
   }
 
   //  var T: Option[Int] = config("T", default = defaultThreads)
@@ -45,31 +44,30 @@ class CleverCaller(val root: Configurable) extends BiopetCommandLineFunction {
   var k: Boolean = config("k", default = false) // keep working directory
   var r: Boolean = config("r", default = false) // take read groups into account
 
-  override def beforeCmd() {
-    if (workdir == null) throw new Exception("Clever :: Workdirectory is not defined")
-    //    if (input.getName.endsWith(".sort.bam")) sorted = true
+  override def beforeGraph() {
+    super.beforeGraph()
+    if (workDir == null) throw new Exception("Clever :: Workdirectory is not defined")
+    if (reference == null) reference = referenceFasta()
   }
 
   def cmdLine = required(executable) +
     " --sorted " +
     " --use_xa " +
-    optional("-T", nCoresRequest) +
+    optional("-T", threads) +
     conditional(f, "-f") +
     conditional(a, "-a") +
     conditional(k, "-k") +
     conditional(r, "-r") +
-    required(this.input) +
-    required(this.reference) +
-    required(this.workdir)
+    required(input) +
+    required(reference) +
+    required(workDir)
 }
 
 object CleverCaller {
-  def apply(root: Configurable, input: File, reference: File, svDir: String, runDir: String): CleverCaller = {
+  def apply(root: Configurable, input: File, svDir: File): CleverCaller = {
     val clever = new CleverCaller(root)
     clever.input = input
-    clever.reference = reference
     clever.cwd = svDir
-    clever.workdir = runDir
     clever
   }
 }
\ No newline at end of file

public/biopet-framework/src/main/scala/nl/lumc/sasc/biopet/extensions/delly/Delly.scala

@@ -2,33 +2,34 @@ package nl.lumc.sasc.biopet.extensions.delly
 
 import java.io.File
 
-import nl.lumc.sasc.biopet.core.{ BiopetQScript, PipelineCommand }
+import nl.lumc.sasc.biopet.core.{ Reference, BiopetQScript, PipelineCommand }
 import nl.lumc.sasc.biopet.core.config.Configurable
 import nl.lumc.sasc.biopet.extensions.Ln
 import org.broadinstitute.gatk.queue.QScript
 import org.broadinstitute.gatk.queue.extensions.gatk.CatVariants
 
-class Delly(val root: Configurable) extends QScript with BiopetQScript {
+class Delly(val root: Configurable) extends QScript with BiopetQScript with Reference {
   def this() = this(null)
 
   @Input(doc = "Input file (bam)")
   var input: File = _
 
-  @Argument(doc = "Work directory")
-  var workdir: String = _
+  var workDir: File = _
 
   @Output(doc = "Delly result VCF")
-  var outputvcf: File = _
+  var outputVcf: File = _
 
-  var outputBaseName: String = _
+  var outputName: String = _
 
   // select the analysis types DEL,DUP,INV,TRA
-  var del: Boolean = config("DEL", default = false)
-  var dup: Boolean = config("DUP", default = false)
-  var inv: Boolean = config("INV", default = false)
-  var tra: Boolean = config("TRA", default = false)
-
-  override def init() {
+  var del: Boolean = config("DEL", default = true)
+  var dup: Boolean = config("DUP", default = true)
+  var inv: Boolean = config("INV", default = true)
+  var tra: Boolean = config("TRA", default = true)
+
+  override def init(): Unit = {
+    if (outputName == null) outputName = input.getName.stripSuffix(".bam")
+    if (outputVcf == null) outputVcf = new File(workDir, outputName + ".delly.vcf")
   }
 
   def biopetScript() {
@@ -37,15 +38,12 @@ class Delly(val root: Configurable) extends QScript with BiopetQScript {
     var outputFiles: Map[String, File] = Map()
     var vcfFiles: Map[String, File] = Map()
 
-    this.outputBaseName = workdir + input.getName.substring(0, input.getName.lastIndexOf(".bam"))
-    this.outputvcf = outputBaseName + ".delly.vcf"
-
     /// start delly and then copy the vcf into the root directory "<sample>.delly/"
     if (del) {
       val delly = new DellyCaller(this)
       delly.input = input
       delly.analysistype = "DEL"
-      delly.outputvcf = outputBaseName + ".delly.del.vcf"
+      delly.outputvcf = new File(workDir, outputName + ".delly.del.vcf")
       add(delly)
       vcfFiles += ("DEL" -> delly.outputvcf)
     }
@@ -53,7 +51,7 @@ class Delly(val root: Configurable) extends QScript with BiopetQScript {
       val delly = new DellyCaller(this)
       delly.input = input
       delly.analysistype = "DUP"
-      delly.outputvcf = outputBaseName + ".delly.dup.vcf"
+      delly.outputvcf = new File(workDir, outputName + ".delly.dup.vcf")
       add(delly)
       vcfFiles += ("DUP" -> delly.outputvcf)
     }
@@ -61,7 +59,7 @@ class Delly(val root: Configurable) extends QScript with BiopetQScript {
       val delly = new DellyCaller(this)
       delly.input = input
       delly.analysistype = "INV"
-      delly.outputvcf = outputBaseName + ".delly.inv.vcf"
+      delly.outputvcf = new File(workDir, outputName + ".delly.inv.vcf")
       add(delly)
       vcfFiles += ("INV" -> delly.outputvcf)
     }
@@ -69,47 +67,43 @@ class Delly(val root: Configurable) extends QScript with BiopetQScript {
       val delly = new DellyCaller(this)
       delly.input = input
       delly.analysistype = "TRA"
-      delly.outputvcf = outputBaseName + ".delly.tra.vcf"
+      delly.outputvcf = new File(workDir, outputName + ".delly.tra.vcf")
       //      vcfFiles += ("TRA" -> delly.outputvcf)
       add(delly)
     }
     // we need to merge the vcf's
-    var finalVCF = if (vcfFiles.size > 1) {
+    val finalVCF = if (vcfFiles.size > 1) {
       // do merging
       // CatVariants is a $org.broadinstitute.gatk.utils.commandline.CommandLineProgram$;
+      //TODO: convert to biopet extension
       val variants = new CatVariants()
-      variants.jobResourceRequests :+= "h_vmem=4G"
-      variants.nCoresRequest = 1
-      variants.memoryLimit = Option(2.0)
       variants.variant = vcfFiles.values.toList
-      variants.reference = config("reference")
-      variants.outputFile = this.outputvcf
+      variants.outputFile = this.outputVcf
+      variants.reference = referenceFasta()
       // add the job
-      add(variants)
-      this.outputvcf
-    } else {
+      //add(variants)
+      Some(outputVcf)
+    } else if (vcfFiles.size == 1) {
       // TODO: pretify this
-      val ln = Ln(this, vcfFiles.head._2, this.outputvcf, relative = true)
-      add(ln)
-      ln.output
-    }
+      val ln = Ln(this, vcfFiles.head._2, this.outputVcf, relative = true)
+      //add(ln)
+      Some(ln.output)
+    } else None
 
-    outputFiles += ("vcf" -> this.outputvcf)
+    finalVCF.foreach(file => outputFiles += ("vcf" -> file))
   }
-
-  private def swapExtension(inputFile: String) = inputFile.substring(0, inputFile.lastIndexOf(".bam")) + ".delly.vcf"
 }
 
 object Delly extends PipelineCommand {
   override val pipeline = "/nl/lumc/sasc/biopet/extensions/svcallers/Delly/Delly.class"
 
-  def apply(root: Configurable, input: File, runDir: String): Delly = {
-    val dellypipeline = new Delly(root)
-    dellypipeline.input = input
-    dellypipeline.workdir = runDir
-    dellypipeline.init()
-    dellypipeline.biopetScript()
-    dellypipeline
+  def apply(root: Configurable, input: File, workDir: File): Delly = {
+    val dellyPipeline = new Delly(root)
+    dellyPipeline.input = input
+    dellyPipeline.workDir = workDir
+    dellyPipeline.init()
+    dellyPipeline.biopetScript()
+    dellyPipeline
   }
 
 }
\ No newline at end of file

public/biopet-framework/src/main/scala/nl/lumc/sasc/biopet/extensions/delly/DellyCaller.scala

@@ -12,6 +12,7 @@ class DellyCaller(val root: Configurable) extends BiopetCommandLineFunction {
   private lazy val versionexecutable: File = new File(executable)
 
   override def defaultThreads = 1
+  override def defaultCoreMemory = 4.0
 
   override def versionCommand = versionexecutable.getAbsolutePath
   override def versionRegex = """DELLY \(Version: (.*)\)""".r

public/biopet-framework/src/main/scala/nl/lumc/sasc/biopet/tools/VcfFilter.scala

@@ -42,7 +42,7 @@ class VcfFilter(val root: Configurable) extends ToolCommandFuntion {
   var minSamplesPass: Option[Int] = config("min_samples_pass")
   var filterRefCalls: Boolean = config("filter_ref_calls", default = false)
 
-  override def defaultCoreMemory = 1.0
+  override def defaultCoreMemory = 3.0
 
   override def commandLine = super.commandLine +
     required("-I", inputVcf) +

public/biopet-framework/src/main/scala/nl/lumc/sasc/biopet/utils/ConfigUtils.scala

@@ -267,6 +267,22 @@ object ConfigUtils extends Logging {
     any2list(any).map(_.toString)
   }
 
+  /** Convert Any to List[Any], fallback on list with 1 value */
+  def any2set(any: Any): Set[Any] = {
+    if (any == null) return null
+    any match {
+      case s: Set[_]  => s.toSet
+      case l: List[_] => l.toSet
+      case _          => Set(any)
+    }
+  }
+
+  /** Convert Any to List[String] */
+  def any2stringSet(any: Any): Set[String] = {
+    if (any == null) return null
+    any2set(any).map(_.toString)
+  }
+
   /** Convert Any to List[File] */
   def any2fileList(any: Any): List[File] = {
     if (any == null) return null
@@ -414,7 +430,7 @@ object ConfigUtils extends Logging {
 
     /** Convert ConfigValue to Set[String] */
     implicit def configValue2stringSet(value: ConfigValue): Set[String] = {
-      if (requiredValue(value)) any2stringList(value.value).toSet
+      if (requiredValue(value)) any2stringSet(value.value)
       else Set()
     }
 

public/biopet-public-package/src/main/scala/nl/lumc/sasc/biopet/core/BiopetExecutablePublic.scala

@@ -24,7 +24,8 @@ object BiopetExecutablePublic extends BiopetExecutable {
     nl.lumc.sasc.biopet.pipelines.sage.Sage,
     nl.lumc.sasc.biopet.pipelines.bamtobigwig.Bam2Wig,
     nl.lumc.sasc.biopet.pipelines.carp.Carp,
-    nl.lumc.sasc.biopet.pipelines.toucan.Toucan
+    nl.lumc.sasc.biopet.pipelines.toucan.Toucan,
+    nl.lumc.sasc.biopet.pipelines.shiva.ShivaSvCalling
   )
 
   def pipelines: List[MainCommand] = List(

public/pom.xml

@@ -33,7 +33,7 @@
         <module>mapping</module>
         <module>sage</module>
         <module>kopisu</module>
-        <module>yamsvp</module>
+        <!-- <module>yamsvp</module> -->
         <module>gears</module>
         <module>bam2wig</module>
         <module>carp</module>

public/shiva/src/main/scala/nl/lumc/sasc/biopet/pipelines/shiva/ShivaSvCalling.scala

+/**
+ * Biopet is built on top of GATK Queue for building bioinformatic
+ * pipelines. It is mainly intended to support LUMC SHARK cluster which is running
+ * SGE. But other types of HPC that are supported by GATK Queue (such as PBS)
+ * should also be able to execute Biopet tools and pipelines.
+ *
+ * Copyright 2014 Sequencing Analysis Support Core - Leiden University Medical Center
+ *
+ * Contact us at: sasc@lumc.nl
+ *
+ * A dual licensing mode is applied. The source code within this project that are
+ * not part of GATK Queue is freely available for non-commercial use under an AGPL
+ * license; For commercial users or users who do not want to follow the AGPL
+ * license, please contact us to obtain a separate license.
+ */
+package nl.lumc.sasc.biopet.pipelines.shiva
+
+import java.io.File
+
+import htsjdk.samtools.SamReaderFactory
+import nl.lumc.sasc.biopet.core.config.Configurable
+import nl.lumc.sasc.biopet.core.summary.SummaryQScript
+import nl.lumc.sasc.biopet.core.{ PipelineCommand, BiopetQScript, Reference, SampleLibraryTag }
+import nl.lumc.sasc.biopet.extensions.breakdancer.Breakdancer
+import nl.lumc.sasc.biopet.extensions.clever.CleverCaller
+import nl.lumc.sasc.biopet.extensions.delly.Delly
+import nl.lumc.sasc.biopet.tools.VcfStats
+import org.broadinstitute.gatk.queue.QScript
+import org.broadinstitute.gatk.utils.commandline.Input
+import scala.collection.JavaConversions._
+
+/**
+ * Common trait for ShivaVariantcalling
+ *
+ * Created by pjvan_thof on 2/26/15.
+ */
+class ShivaSvCalling(val root: Configurable) extends QScript with SummaryQScript with SampleLibraryTag with Reference {
+  qscript =>
+
+  def this() = this(null)
+
+  @Input(doc = "Bam files (should be deduped bams)", shortName = "BAM", required = true)
+  protected var inputBamsArg: List[File] = Nil
+
+  protected var inputBams: Map[String, File] = Map()
+
+  def addBamFile(file: File, sampleId: Option[String] = None): Unit = {
+    sampleId match {
+      case Some(sample)        => inputBams += sample -> file
+      case _ if !file.exists() => throw new IllegalArgumentException("Bam file does not exits: " + file)
+      case _ => {
+        val inputSam = SamReaderFactory.makeDefault.open(file)
+        val samples = inputSam.getFileHeader.getReadGroups.map(_.getSample).distinct
+        if (samples.size == 1) {
+          inputBams += samples.head -> file
+        } else throw new IllegalArgumentException("Bam contains multiple sample IDs: " + file)
+      }
+    }
+  }
+
+  /** Executed before script */
+  def init(): Unit = {
+    inputBamsArg.foreach(addBamFile(_))
+  }
+
+  /** Variantcallers requested by the config */
+  protected val configCallers: Set[String] = config("sv_callers", default = Set("breakdancer", "clever", "delly"))
+
+  /** This will add jobs for this pipeline */
+  def biopetScript(): Unit = {
+    for (cal <- configCallers) {
+      if (!callersList.exists(_.name == cal))
+        BiopetQScript.addError("variantcaller '" + cal + "' does not exist, possible to use: " + callersList.map(_.name).mkString(", "))
+    }
+
+    val callers = callersList.filter(x => configCallers.contains(x.name))
+
+    require(inputBams.nonEmpty, "No input bams found")
+    require(callers.nonEmpty, "must select at least 1 SV caller, choices are: " + callersList.map(_.name).mkString(", "))
+
+    callers.foreach(_.addJobs())
+
+    addSummaryJobs()
+  }
+
+  /** Will generate all available variantcallers */
+  protected def callersList: List[SvCaller] = List(new Breakdancer, new Clever, new Delly)
+
+  /** General trait for a variantcaller mode */
+  trait SvCaller {
+    /** Name of mode, this should also be used in the config */
+    val name: String
+
+    /** Output dir for this mode */
+    def outputDir = new File(qscript.outputDir, name)
+
+    /** This should add the variantcaller jobs */
+    def addJobs()