Skip to content
Snippets Groups Projects
Commit 731d6089 authored by sajvanderzeeuw's avatar sajvanderzeeuw
Browse files

added start up sage docs

parent 1a209ea0
No related branches found
No related tags found
No related merge requests found
......@@ -4,7 +4,7 @@
The GATK-pipeline is build for variant calling on NGS data (preferably Illumina data).
It is based on the <a href="https://www.broadinstitute.org/gatk/guide/best-practices" target="_blank">best practices</a>) of GATK in terms of there approach to variant calling.
The pipeline accepts ```.fastq & .bam``` files as <samplename>.
The pipeline accepts ```.fastq & .bam``` files as input.
----
......
# Introduction
The Sage pipeline has been created to process SAGE data, which requires a different approach than NGS data.
The Sage pipeline has been created to process SAGE data, which requires a different approach than standard NGS data.
# Tools for this pipeline
* [Flexiprep](flexiprep.md)
* [Mapping](mapping.md)
* [SageCountFastq](sagetools.md)
* [SageCreateLibrary](sagetools.md)
* [SageCreateTagCounts](sagetools.md)
# Invocation
# Example
Note that one should first create the appropriate [configs](../config.md).
~~~
java -jar Biopet-0.2.0-DEV-801b72ed.jar pipeline Sage -h
java -jar Biopet-0.2.0.jar pipeline Sage -h
Arguments for Sage:
-outDir,--output_directory <output_directory> Output directory
--countbed <countbed> countBed
......@@ -20,13 +26,70 @@ Arguments for Sage:
~~~
# Testcase A
# Testcase B
# Examine results
## Result files
~~~
.
├── 1A
│ ├── 1A-2.merge.bai
│ ├── 1A-2.merge.bam
│ ├── 1A.fastq
│ ├── 1A.genome.antisense.counts
│ ├── 1A.genome.antisense.coverage
│ ├── 1A.genome.counts
│ ├── 1A.genome.coverage
│ ├── 1A.genome.sense.counts
│ ├── 1A.genome.sense.coverage
│ ├── 1A.raw.counts
│ ├── 1A.tagcount.all.antisense.counts
│ ├── 1A.tagcount.all.sense.counts
│ ├── 1A.tagcount.antisense.counts
│ ├── 1A.tagcount.sense.counts
│ ├── run_1
│ │ ├── 1A-1.bai
│ │ ├── 1A-1.bam
│ │ ├── flexiprep
│ │ └── metrics
│ └── run_2
│ ├── 1A-2.bai
│ ├── 1A-2.bam
│ ├── flexiprep
│ └── metrics
├── 1B
│ ├── 1B-2.merge.bai
│ ├── 1B-2.merge.bam
│ ├── 1B.fastq
│ ├── 1B.genome.antisense.counts
│ ├── 1B.genome.antisense.coverage
│ ├── 1B.genome.counts
│ ├── 1B.genome.coverage
│ ├── 1B.genome.sense.counts
│ ├── 1B.genome.sense.coverage
│ ├── 1B.raw.counts
│ ├── 1B.tagcount.all.antisense.counts
│ ├── 1B.tagcount.all.sense.counts
│ ├── 1B.tagcount.antisense.counts
│ ├── 1B.tagcount.sense.counts
│ ├── run_1
│ │ ├── 1B-1.bai
│ │ ├── 1B-1.bam
│ │ ├── flexiprep
│ │ └── metrics
│ └── run_2
│ ├── 1B-2.bai
│ ├── 1B-2.bam
│ ├── flexiprep
│ └── metrics
├── ensgene.squish.bed
├── summary-33.tsv
├── taglib
├── no_antisense_genes.txt
├── no_sense_genes.txt
└── tag.lib
~~~
## Best practice
......
......@@ -11,13 +11,13 @@ so usually one does not want to safe these files.
## Example
To start the tool:
~~~
java -jar Biopet-0.2.0-DEV-801b72ed.jar tool mpileupToVcf
java -jar Biopet-0.2.0.jar tool mpileupToVcf
~~~
To open the help:
~~~bash
java -jar Biopet-0.2.0-DEV-801b72ed.jar tool mpileupToVcf -h
java -jar Biopet-0.2.0.jar tool mpileupToVcf -h
Usage: MpileupToVcf [options]
-l <value> | --log_level <value>
......
# SAGE tools
## SageCountFastq
~~~
Usage: SageCountFastq [options]
-l <value> | --log_level <value>
Log level
-h | --help
Print usage
-v | --version
Print version
-I <file> | --input <file>
-o <file> | --output <file>
~~~
## SageCreateLibrary
~~~
Usage: SageCreateLibrary [options]
-l <value> | --log_level <value>
Log level
-h | --help
Print usage
-v | --version
Print version
-I <file> | --input <file>
-o <file> | --output <file>
--tag <value>
--length <value>
--noTagsOutput <file>
--noAntiTagsOutput <file>
--allGenesOutput <file>
~~~
## SageCreateTagCounts
~~~
Usage: SageCreateTagCounts [options]
-l <value> | --log_level <value>
Log level
-h | --help
Print usage
-v | --version
Print version
-I <file> | --input <file>
-t <file> | --tagLib <file>
--countSense <file>
--countAllSense <file>
--countAntiSense <file>
--countAllAntiSense <file>
~~~
\ No newline at end of file
......@@ -9,6 +9,8 @@ pages:
- ['pipelines/sage.md', 'Pipelines', 'Sage']
- ['tools/SamplesTsvToJson.md','tools','SamplesTsvToJson']
- ['tools/BastyGenerateFasta.md','tools','BastyGenerateFasta']
- ['tools/MpileupToVcf.md', 'tools', 'MpileupToVcf']
- ['tools/sagetools.md', 'tools', 'Sagetools']
- ['cluster/oge.md', 'OpenGridEngine']
- ['about.md', 'About']
- ['license.md', 'License']
......
0% Loading or .
You are about to add 0 people to the discussion. Proceed with caution.
Finish editing this message first!
Please register or to comment