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Commit 482ffc59 authored by Wai Yi Leung's avatar Wai Yi Leung
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Remove the indexing requirement from sambamba, since this does this on the fly since version v0.5.0

parent bd2f00b0
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......@@ -74,7 +74,13 @@ class Yamsvp(val root: Configurable) extends QScript with MultiSampleQScript {
}
val bamFile: File =
if (libraryBamfiles.size == 1) libraryBamfiles.head
if (libraryBamfiles.size == 1) {
// When the sample has only 1 run, make a link in the main alignment directory
val alignmentlink = Ln( root, libraryBamfiles.head,
alignmentDir + sampleID + ".merged.bam", true )
add(alignmentlink, isIntermediate = true)
alignmentlink.out
}
else if (libraryBamfiles.size > 1) {
val mergeSamFiles = new SambambaMerge(root)
mergeSamFiles.input = libraryBamfiles
......@@ -83,21 +89,18 @@ class Yamsvp(val root: Configurable) extends QScript with MultiSampleQScript {
mergeSamFiles.output
} else null
// TODO: Check whether sambamba reaches release version v0.5.0 which includes
// automatic indexing of the bam file.
val bamMarkDup = SambambaMarkdup(root, bamFile)
add(bamMarkDup)
val analysisBam: File = bamMarkDup.output
val analysisBamIndex = SambambaIndex(root, analysisBam)
add(analysisBamIndex)
// val analysisBam: File = bamMarkDup.output
// val analysisBamIndex = SambambaIndex(root, analysisBam)
// add(analysisBamIndex)
/// bamfile will be used as input for the SV callers. First run Clever
// val cleverVCF : File = sampleDir + "/" + sampleID + ".clever.vcf"
val cleverDir = svcallingDir + sampleID + ".clever/"
val clever = CleverCaller(this, analysisBam, this.reference, svcallingDir, cleverDir)
clever.deps = List(analysisBamIndex.output)
val clever = CleverCaller(this, bamMarkDup.output, this.reference, svcallingDir, cleverDir)
sampleOutput.vcf += ("clever" -> List(clever.outputvcf))
add(clever)
......@@ -105,7 +108,7 @@ class Yamsvp(val root: Configurable) extends QScript with MultiSampleQScript {
add(clever_vcf)
val breakdancerDir = svcallingDir + sampleID + ".breakdancer/"
val breakdancer = Breakdancer(this, analysisBam, this.reference, breakdancerDir)
val breakdancer = Breakdancer(this, bamMarkDup.output, this.reference, breakdancerDir)
sampleOutput.vcf += ("breakdancer" -> List(breakdancer.outputvcf))
addAll(breakdancer.functions)
......@@ -113,7 +116,7 @@ class Yamsvp(val root: Configurable) extends QScript with MultiSampleQScript {
add(bd_vcf)
val dellyDir = svcallingDir + sampleID + ".delly/"
val delly = Delly(this, analysisBam, dellyDir)
val delly = Delly(this, bamMarkDup.output, dellyDir)
sampleOutput.vcf += ("delly" -> List(delly.outputvcf))
addAll(delly.functions)
......
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