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Commit 1155ac47 authored by van den Berg's avatar van den Berg
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Deprecate fastq_count

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...@@ -142,7 +142,6 @@ The following configuration options are **optional**: ...@@ -142,7 +142,6 @@ The following configuration options are **optional**:
| ------------- | ----------- | | ------------- | ----------- |
| `BED` | Comma-separate list of paths to BED files of interest | | `BED` | Comma-separate list of paths to BED files of interest |
| `FEMALE_THRESHOLD` | Float between 0 and 1 that signifies the threshold of the ratio between coverage on X/overall coverage that 'calls' a sample as female. Default = 0.6 | | `FEMALE_THRESHOLD` | Float between 0 and 1 that signifies the threshold of the ratio between coverage on X/overall coverage that 'calls' a sample as female. Default = 0.6 |
| `FASTQ_COUNT` | Path to `fastq-count` executable |
| `MAX_BASES` | Maximum allowed number of bases per sample before subsampling. Default = None (no subsampling) | | `MAX_BASES` | Maximum allowed number of bases per sample before subsampling. Default = None (no subsampling) |
| `KNOWN_SITES` | Path to one or more VCF files of known variants, to be used with baserecalibration | | `KNOWN_SITES` | Path to one or more VCF files of known variants, to be used with baserecalibration |
...@@ -208,7 +207,6 @@ snakemake -s Snakefile \ ...@@ -208,7 +207,6 @@ snakemake -s Snakefile \
REFERENCE=/path/to/genome.fasta \ REFERENCE=/path/to/genome.fasta \
KNOWN_SITES=/path/to/dbsnp.vcf.gz,/path/to/onekg.vcf,/path/to/hapmap.vcf \ KNOWN_SITES=/path/to/dbsnp.vcf.gz,/path/to/onekg.vcf,/path/to/hapmap.vcf \
DBSNP=/path/to/dbsnp.vcf.gz \ DBSNP=/path/to/dbsnp.vcf.gz \
FASTQ_COUNT=/path/to/fastq-count \
BED=/path/to/interesting_region.bed BED=/path/to/interesting_region.bed
``` ```
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...@@ -62,7 +62,6 @@ for filename in KNOWN_SITES: ...@@ -62,7 +62,6 @@ for filename in KNOWN_SITES:
BED = config.get("BED", "") # comma-separated list of BED files BED = config.get("BED", "") # comma-separated list of BED files
REFFLAT = config.get("REFFLAT", "") # comma-separated list of refFlat files REFFLAT = config.get("REFFLAT", "") # comma-separated list of refFlat files
FEMALE_THRESHOLD = config.get("FEMALE_THRESHOLD", 0.6) FEMALE_THRESHOLD = config.get("FEMALE_THRESHOLD", 0.6)
FASTQ_COUNT = config.get("FASTQ_COUNT")
MAX_BASES = config.get("MAX_BASES", "") MAX_BASES = config.get("MAX_BASES", "")
# Generate the input string for basrecalibration # Generate the input string for basrecalibration
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