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NGS-intro-course
Commits
1eb792d1
Commit
1eb792d1
authored
10 years ago
by
Michiel van Galen
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Added links to html output
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1295302e
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practical_one/practical_one.tex
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1eb792d1
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@@ -305,6 +305,13 @@ This will create a folder with data and plots of the analysis. Have a look at
the fastq
\_
data.txt file to get an idea of the metrics of this set of reads.
\medskip
FastQC also generates an HTML file. We have put the output somewhere online
already for you. In your browser visit the next url:
\medskip
https:
//
barmsijs.lumc.nl
/
NGS
-
course
/
course.fq
\_
fastqc
/
fastqc
\_
report.html
\medskip
% Alignment
\subsection
{
Alignment with Bowtie
}
We skip the clipping process and continue with the alignment. Run Bowtie, same
...
...
@@ -345,7 +352,7 @@ arguments come first before the others. (As you can see in the help)
\item
Run a Bowtie analysis on the data by aligning to the mtDNA index:
\end{itemize}
\begin{lstlisting}
$
bowtie
-
S .
/
ref
/
chrM course.fq course.sam
$
bowtie
-
S .
/
bowtie
_
index
/
chrM course.fq course.sam
\end
{
lstlisting
}
\medskip
...
...
@@ -446,6 +453,13 @@ same time expand annotation for interesting variants only. Manage this using
the next parameters.
\medskip
Like FastQC, VEP also creates an HTML file. We have put this online for you. If
you like to have a look at this, open a browser and visit:
\medskip
https://barmsijs.lumc.nl/NGS-course/course.fq.vep
\_
summary.html
\medskip
\begin{itemize}
\item
Try some of these parameters and figure out what they do:
\item
-
{}
-check
\_
existing -
{}
-canonical -
{}
-coding
\_
only -
{}
-force
\_
overwrite
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