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Commit 83256df2 authored by JB's avatar JB
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Fix conflicts with remote

parents d42b3593 452d7a50
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......@@ -12,6 +12,12 @@ that users understand how the changes affect the new version.
version 2.1.0-dev
---------------------------
+ Updated TALON to version 4.4
+ Added parameter_meta sections to the following tools:
+ htseq
+ cutadapt
+ collect-columns
+ stringtie
+ fastqc
+ Updated star default image to 2.7.3a
+ Hisat2 now indexes the resulting BAM file.
+ Samtools index now also works without setting a path for the output
......
......@@ -104,7 +104,7 @@ task Kit {
category: "required"
}
outputPrefix: {
description: "The prefix of the output files, including any parent directories",
description: "The prefix of the output files, including any parent directories.",
category: "required"
}
readgroup: {
......@@ -128,7 +128,7 @@ task Kit {
category: "advanced"
}
dockerImage: {
description: "The docker image used for this task. Changing this may result in errors which the developers may chose not to address.",
description: "The docker image used for this task. Changing this may result in errors which the developers may choose not to address.",
category: "advanced"
}
......
......@@ -42,4 +42,51 @@ task CollectColumns {
memory: "~{memoryGb}G"
docker: dockerImage
}
parameter_meta {
inputTables: {
description: "The tables from which columns should be taken.",
category: "required"
}
outputPath: {
description: "The path to which the output should be written.",
category: "required"
}
featureColumn: {
description: "Equivalent to the -f option of collect-columns.",
category: "common" # Should likely be controlled by the calling workflow
}
valueColumn: {
description: "Equivalent to the -c option of collect-columns.",
category: "common" # Should likely be controlled by the calling workflow
}
separator: {
description: "Equivalent to the -s option of collect-columns.",
category: "common" # Should likely be controlled by the calling workflow
}
sampleNames: {
description: "Equivalent to the -n option of collect-columns.",
category: "common" # Should likely be controlled by the calling workflow
}
header: {
description: "Equivalent to the -H flag of collect-columns.",
category: "common"
}
additionalAttributes: {
description: "Equivalent to the -a option of collect-columns.",
category: "advanced"
}
referenceGtf: {
description: "Equivalent to the -g option of collect-columns.",
category: "advanced"
}
featureAttribute: {
description: "Equivalent to the -F option of collect-columns.",
category: "advanced"
}
dockerImage: {
description: "The docker image used for this task. Changing this may result in errors which the developers may choose not to address.",
category: "advanced"
}
}
}
\ No newline at end of file
......@@ -116,7 +116,7 @@ task Cutadapt {
~{true="--no-trim" false="" noTrim} \
~{true="--mask-adapter" false="" maskAdapter} \
~{true="--no-indels" false="" noIndels} \
~{true="--trim-n" false="" trimN} \
~{true="--trim-n" false="" trimN} \
~{true="--interleaved" false="" interleaved} \
~{true="--discard-trimmed" false="" discardTrimmed } \
~{true="--colorspace" false="" colorspace} \
......@@ -151,4 +151,239 @@ task Cutadapt {
memory: memory
docker: dockerImage
}
parameter_meta {
read1: {
description: "The first or single end fastq file to be run through cutadapt.",
category: "required"
}
read2: {
description: "An optional second end fastq file to be run through cutadapt.",
category: "common"
}
read1output: {
description: "The name of the resulting first or single end fastq file.",
category: "common"
}
read2output: {
description: "The name of the resulting second end fastq file.",
category: "common"
}
format: {
description: "Equivalent to cutadapt's --format option.",
category: "advanced"
}
adapter: {
description: "A list of 3' ligated adapter sequences to be cut from the given first or single end fastq file.",
category: "common"
}
front: {
description: "A list of 5' ligated adapter sequences to be cut from the given first or single end fastq file.",
category: "advanced"
}
anywhere: {
description: "A list of 3' or 5' ligated adapter sequences to be cut from the given first or single end fastq file.",
category: "advanced"
}
adapterRead2: {
description: "A list of 3' ligated adapter sequences to be cut from the given second end fastq file.",
category: "common"
}
frontRead2: {
description: "A list of 5' ligated adapter sequences to be cut from the given second end fastq file.",
category: "advanced"
}
anywhereRead2: {
description: "A list of 3' or 5' ligated adapter sequences to be cut from the given second end fastq file.",
category: "advanced"
}
interleaved: {
description: "Equivalent to cutadapt's --interleaved flag.",
category: "advanced"
}
pairFilter: {
description: "Equivalent to cutadapt's --pair-filter option.",
category: "advanced"
}
errorRate: {
description: "Equivalent to cutadapt's --error-rate option.",
category: "advanced"
}
noIndels: {
description: "Equivalent to cutadapt's --no-indels flag.",
category: "advanced"
}
times: {
description: "Equivalent to cutadapt's --times option.",
category: "advanced"
}
overlap: {
description: "Equivalent to cutadapt's --overlap option.",
category: "advanced"
}
matchReadWildcards: {
description: "Equivalent to cutadapt's --match-read-wildcards flag.",
category: "advanced"
}
noMatchAdapterWildcards: {
description: "Equivalent to cutadapt's --no-match-adapter-wildcards flag.",
category: "advanced"
}
noTrim: {
description: "Equivalent to cutadapt's --no-trim flag.",
category: "advanced"
}
maskAdapter: {
description: "Equivalent to cutadapt's --mask-adapter flag.",
category: "advanced"
}
cut: {
description: "Equivalent to cutadapt's --cut option.",
category: "advanced"
}
nextseqTrim: {
description: "Equivalent to cutadapt's --nextseq-trim option.",
category: "advanced"
}
qualityCutoff: {
description: "Equivalent to cutadapt's --quality-cutoff option.",
category: "advanced"
}
qualityBase: {
description: "Equivalent to cutadapt's --quality-base option.",
category: "advanced"
}
length: {
description: "Equivalent to cutadapt's --length option.",
category: "advanced"
}
trimN: {
description: "Equivalent to cutadapt's --trim-n flag.",
category: "advanced"
}
lengthTag: {
description: "Equivalent to cutadapt's --length-tag option.",
category: "advanced"
}
stripSuffix: {
description: "Equivalent to cutadapt's --strip-suffix option.",
category: "advanced"
}
prefix: {
description: "Equivalent to cutadapt's --prefix option.",
category: "advanced"
}
suffix: {
description: "Equivalent to cutadapt's --suffix option.",
category: "advanced"
}
minimumLength: {
description: "Equivalent to cutadapt's --minimum-length option.",
category: "advanced"
}
maximumLength: {
description: "Equivalent to cutadapt's --maximum-length option.",
category: "advanced"
}
maxN: {
description: "Equivalent to cutadapt's --max-n option.",
category: "advanced"
}
discardTrimmed: {
description: "Equivalent to cutadapt's --quality-cutoff option.",
category: "advanced"
}
discardUntrimmed: {
description: "Equivalent to cutadapt's --discard-untrimmed option.",
category: "advanced"
}
infoFilePath: {
description: "Equivalent to cutadapt's --info-file option.",
category: "advanced"
}
restFilePath: {
description: "Equivalent to cutadapt's --rest-file option.",
category: "advanced"
}
wildcardFilePath: {
description: "Equivalent to cutadapt's --wildcard-file option.",
category: "advanced"
}
tooShortOutputPath: {
description: "Equivalent to cutadapt's --too-short-output option.",
category: "advanced"
}
tooLongOutputPath: {
description: "Equivalent to cutadapt's --too-long-output option.",
category: "advanced"
}
untrimmedOutputPath: {
description: "Equivalent to cutadapt's --untrimmed-output option.",
category: "advanced"
}
tooShortPairedOutputPath: {
description: "Equivalent to cutadapt's --too-short-paired-output option.",
category: "advanced"
}
tooLongPairedOutputPath: {
description: "Equivalent to cutadapt's --too-long-paired-output option.",
category: "advanced"
}
untrimmedPairedOutputPath: {
description: "Equivalent to cutadapt's --untrimmed-paired-output option.",
category: "advanced"
}
colorspace: {
description: "Equivalent to cutadapt's --colorspace flag.",
category: "advanced"
}
doubleEncode: {
description: "Equivalent to cutadapt's --double-encode flag.",
category: "advanced"
}
trimPrimer: {
description: "Equivalent to cutadapt's --trim-primer flag.",
category: "advanced"
}
stripF3: {
description: "Equivalent to cutadapt's --strip-f3 flag.",
category: "advanced"
}
maq: {
description: "Equivalent to cutadapt's --maq flag.",
category: "advanced"
}
bwa: {
description: "Equivalent to cutadapt's --bwa flag.",
category: "advanced"
}
zeroCap: {
description: "Equivalent to cutadapt's --zero-cap flag.",
category: "advanced"
}
noZeroCap: {
description: "Equivalent to cutadapt's --no-zero-cap flag.",
category: "advanced"
}
reportPath: {
description: "The name of the file to write cutadapts's stdout to, this contains some metrics.",
category: "common"
}
Z: {
description: "Equivalent to cutadapt's -Z flag.",
category: "advanced"
}
cores: {
description: "The number of cores to use.",
category: "advanced"
}
memory: {
description: "The amount of memory this job will use.",
category: "advanced"
}
dockerImage: {
description: "The docker image used for this task. Changing this may result in errors which the developers may choose not to address.",
category: "advanced"
}
}
}
......@@ -63,6 +63,79 @@ task Fastqc {
cpu: threads
docker: dockerImage
}
parameter_meta {
seqFile: {
description: "A fastq file.",
category: "required"
}
outdirPath: {
description: "The path to write the output to",
catgory: "required"
}
casava: {
description: "Equivalent to fastqc's --casava flag.",
category: "advanced"
}
nano: {
description: "Equivalent to fastqc's --nano flag.",
category: "advanced"
}
noFilter: {
description: "Equivalent to fastqc's --nofilter flag.",
category: "advanced"
}
extract: {
description: "Equivalent to fastqc's --extract flag.",
category: "advanced"
}
nogroup: {
description: "Equivalent to fastqc's --nogroup flag.",
category: "advanced"
}
minLength: {
description: "Equivalent to fastqc's --min_length option.",
category: "advanced"
}
format: {
description: "Equivalent to fastqc's --format option.",
category: "advanced"
}
contaminants: {
description: "Equivalent to fastqc's --contaminants option.",
category: "advanced"
}
adapters: {
description: "Equivalent to fastqc's --adapters option.",
category: "advanced"
}
limits: {
description: "Equivalent to fastqc's --limits option.",
category: "advanced"
}
kmers: {
description: "Equivalent to fastqc's --kmers option.",
category: "advanced"
}
dir: {
description: "Equivalent to fastqc's --dir option.",
category: "advanced"
}
threads: {
description: "The number of cores to use.",
category: "advanced"
}
dockerImage: {
description: "The docker image used for this task. Changing this may result in errors which the developers may choose not to address.",
category: "advanced"
}
}
meta {
WDL_AID: {
exclude: ["NoneFile", "NoneArray"]
}
}
}
task GetConfiguration {
......@@ -89,4 +162,11 @@ task GetConfiguration {
memory: "2G" # Needs more than 1 to pull the docker image
docker: dockerImage
}
parameter_meta {
dockerImage: {
description: "The docker image used for this task. Changing this may result in errors which the developers may choose not to address.",
category: "advanced"
}
}
}
......@@ -39,4 +39,51 @@ task HTSeqCount {
memory: memory
docker: dockerImage
}
parameter_meta {
inputBams: {
description: "The input BAM files.",
category: "required"
}
gtfFile: {
description: "A GTF/GFF file containing the features of interest.",
category: "required"
}
outputTable: {
description: "The path to which the output table should be written.",
category: "common"
}
format: {
description: "Equivalent to the -f option of htseq-count.",
category: "advanced"
}
order: {
description: "Equivalent to the -r option of htseq-count.",
category: "advanced"
}
stranded: {
description: "Equivalent to the -s option of htseq-count.",
category: "common"
}
featureType: {
description: "Equivalent to the --type option of htseq-count.",
category: "advanced"
}
idattr: {
description: "Equivalent to the --idattr option of htseq-count.",
category: "advanced"
}
additionalAttributes: {
description: "Equivalent to the --additional-attr option of htseq-count.",
category: "advanced"
}
memory: {
description: "The amount of memory the job requires in GB.",
category: "advanced"
}
dockerImage: {
description: "The docker image used for this task. Changing this may result in errors which the developers may choose not to address.",
category: "advanced"
}
}
}
......@@ -40,6 +40,53 @@ task Stringtie {
memory: memory
docker: dockerImage
}
parameter_meta {
bam: {
description: "The input BAM file.",
category: "required"
}
bamIndex: {
description: "The input BAM file's index.",
category: "required"
}
referenceGtf: {
description: "A reference GTF file to be used as guide.",
category: "common"
}
skipNovelTranscripts: {
description: "Whether new transcripts should be assembled or not.",
category: "common"
}
assembledTranscriptsFile: {
description: "Where the output of the assembly should be written.",
category: "required"
}
firstStranded: {
description: "Equivalent to the --rf flag of stringtie.",
category: "required"
}
secondStranded: {
description: "Equivalent to the --fr flag of stringtie.",
category: "required"
}
geneAbundanceFile: {
description: "Where the abundance file should be written.",
category: "common"
}
threads: {
description: "The number of threads to use.",
category: "advanced"
}
memory: {
description: "The amount of memory needed for this task in GB.",
category: "advanced"
}
dockerImage: {
description: "The docker image used for this task. Changing this may result in errors which the developers may choose not to address.",
category: "advanced"
}
}
}
task Merge {
......@@ -83,4 +130,55 @@ task Merge {
memory: memory
docker: dockerImage
}
parameter_meta {
gtfFiles: {
description: "The GTF files produced by stringtie.",
category: "required"
}
outputGtfPath: {
description: "Where the output should be written.",
category: "required"
}
guideGtf: {
description: "Equivalent to the -G option of 'stringtie --merge'.",
category: "advanced"
}
minimumLength: {
description: "Equivalent to the -m option of 'stringtie --merge'.",
category: "advanced"
}
minimumCoverage: {
description: "Equivalent to the -c option of 'stringtie --merge'.",
category: "advanced"
}
minimumFPKM: {
description: "Equivalent to the -F option of 'stringtie --merge'.",
category: "advanced"
}
minimumTPM: {
description: "Equivalent to the -T option of 'stringtie --merge'.",
category: "advanced"
}
minimumIsoformFraction: {
description: "Equivalent to the -f option of 'stringtie --merge'.",
category: "advanced"
}
keepMergedTranscriptsWithRetainedIntrons: {
description: "Equivalent to the -i flag of 'stringtie --merge'.",
category: "advanced"
}
label: {
description: "Equivalent to the -l option of 'stringtie --merge'.",
category: "advanced"
}
memory: {
description: "The amount of memory needed for this task in GB.",
category: "advanced"
}
dockerImage: {
description: "The docker image used for this task. Changing this may result in errors which the developers may choose not to address.",
category: "advanced"
}
}
}
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