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Commit 47035fb2 authored by Ruben Vorderman's avatar Ruben Vorderman
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added bwa index task

parent e63040a8
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2 merge requests!9Changes for virus assembly pipeline,!10Extra tasks required for assembly.
......@@ -81,7 +81,7 @@ task mem {
${"-I " + I } \
${referenceFile} ${read1} ${read2} \
| picard SortSam CREATE_INDEX=TRUE TMP_DIR=null \
INPUT=/dev/stdin OUTPUT=${outputFile}
INPUT=/dev/stdin SORT_ORDER=coordinate OUTPUT=${outputFile}
}
output {
File alignedFile = select_first([outputFile])
......@@ -90,4 +90,34 @@ task mem {
cpu: select_first([threads])
memory: select_first([memory])
}
}
task index {
File fasta
String? preCommand
String? constructionAlgorithm
Int? blockSize
String? outputDir
String fastaFilename = basename(fasta)
command {
set -e -o pipefail
${"mkdir -p " + outputDir}
${preCommand}
ln -sf ${fasta} ${outputDir + "/"}${fastaFilename}
bwa index \
${"-a " + constructionAlgorithm} \
${"-b" + blockSize} \
${outputDir + "/"}${fastaFilename}
}
output {
File indexedFasta = if (defined(outputDir)) then select_first([outputDir]) + "/" + fastaFilename else fastaFilename
}
parameter_meta {
fasta: "Fasta file to be indexed"
constructionAlgorithm: "-a STR BWT construction algorithm: bwtsw, is or rb2 [auto]"
blockSize: "-b INT block size for the bwtsw algorithm (effective with -a bwtsw) [10000000]"
outputDir: "index will be created in this output directory"
}
}
\ No newline at end of file
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