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Commit 38a76485 authored by JasperBoom's avatar JasperBoom
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Update parameter_meta Lima.wdl.

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......@@ -21,4 +21,26 @@ version 1.0
# SOFTWARE.
task refine {
input {
}
command {
set -e
mkdir -p "$(dirname ~{outputPrefix})"
}
output {
}
runtime {
cpu: cores
memory: memory
docker: dockerImage
}
parameter_meta {
# inputs
# outputs
}
}
......@@ -106,7 +106,42 @@ task lima {
parameter_meta {
# inputs
libraryDesign: {description: "", category: ""}
libraryDesign: {description: "Barcode structure of the library design.", category: "required"}
scoreFullPass: {description: "Only use subreads flanked by adapters for barcode identification.", category: "advanced"}
maxScoredBarcodePairs: {description: "Only use up to N barcode pair regions to find the barcode, 0 means use all.", category: "advanced"}
maxScoredBarcodes: {description: "Analyze at maximum the provided number of barcodes per ZMW, 0 means deactivated.", category: "advanced"}
maxScoredAdapters: {description: "Analyze at maximum the provided number of adapters per ZMW, 0 means deactivated.", category: "advanced"}
minPasses: {description: "Minimal number of full passes.", category: "common"}
minLength: {description: "Minimum sequence length after clipping.", category: "common"}
maxInputLength: {description: "Maximum input sequence length, 0 means deactivated.", category: "advanced"}
minRefSpan: {description: "Minimum reference span relative to the barcode length.", category: "advanced"}
minScoringRegion: {description: "Minimum number of barcode regions with sufficient relative span to the barcode length.", category: "advanced"}
minScore: {description: "Reads below the minimum barcode score are removed from downstream analysis.", category: "common"}
minEndScore: {description: "Minimum end barcode score threshold is applied to the individual leading and trailing ends.", category: "advanced"}
minSignalIncrease: {description: "The minimal score difference, between first and combined, required to call a barcode pair different.", category: "advanced"}
minScoreLead: {description: "The minimal score lead required to call a barcode pair significant.", category: "common"}
ccsMode: {description: "CCS mode, use optimal alignment options.", category: "common"}
splitBamNamed: {description: "Split BAM output by resolved barcode pair name.", category: "common"}
scoredAdapterRatio: {description: "Minimum ratio of scored vs sequenced adapters.", category: "advanced"}
peek: {description: "Demux the first N ZMWs and return the mean score, 0 means peeking deactivated.", category: "advanced"}
guess: {description: "Try to guess the used barcodes, using the provided mean score threshold, 0 means guessing deactivated.", category: "advanced"}
guessMinCount: {description: "Minimum number of ZMWs observed to whitelist barcodes.", category: "advanced"}
peekGuess: {description: "Try to infer the used barcodes subset, by peeking at the first 50,000 ZMWs.", category: "advanced"}
logLevel: {description: "Set log level. Valid choices: (TRACE, DEBUG, INFO, WARN, FATAL).", category: "advanced"}
inputBamFile: {description: "BAM input file.", category: "required"}
barcodeFile: {description: "Barcode fasta file.", category: "required"}
outputPrefix: {description: "Output directory path + output file prefix.", category: "required"}
cores: {description: "The number of cores to be used.", category: "advanced"}
memory: {description: "The amount of memory available to the job.", category: "advanced"}
dockerImage: {description: "The docker image used for this task. Changing this may result in errors which the developers may choose not to address.", category: "advanced"}
# outputs
outputFLfile: {description: "Demultiplexed reads output file."}
outputFLindexFile: {description: "Index of demultiplexed reads output file."}
outputSTDERRfile: {description: "Lima STDERR log file."}
outputJSONfile: {description: "Lima JSON file."}
outputCountsFile: {description: "Lima counts file."}
outputReportFile: {description: "Lima report file."}
outputSummaryFile: {description: "Lima summary file."}
}
}
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