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Commit 185ead0f authored by van den Berg's avatar van den Berg
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Add pbmm2, the PacBio wrapper for minimap2

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version 1.0
# Copyright (c) 2020 Leiden University Medical Center
#
# Permission is hereby granted, free of charge, to any person obtaining a copy
# of this software and associated documentation files (the "Software"), to deal
# in the Software without restriction, including without limitation the rights
# to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
# copies of the Software, and to permit persons to whom the Software is
# furnished to do so, subject to the following conditions:
# The above copyright notice and this permission notice shall be included in all
# copies or substantial portions of the Software.
# THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
# IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
# FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
# AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
# LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
# OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
# SOFTWARE.
task Mapping {
input {
String presetOption
Boolean sort=true
String sample
File referenceMMI
File queryFile
Int cores = 4
String memory = "30G"
Int timeMinutes = 1 + ceil(size(queryFile, "G") * 200 / cores)
String dockerImage = "quay.io/biocontainers/pbmm2:1.3.0--h56fc30b_1"
}
command {
set -e
pbmm2 align \
--preset ~{presetOption} \
~{true="--sort" false="" sort} \
-j ~{cores} \
~{referenceMMI} \
~{queryFile} \
~{sample}.align.bam
}
output {
File outputAlignmentFile = sample + ".align.bam"
}
runtime {
cpu: cores
memory: memory
time_minutes: timeMinutes
docker: dockerImage
}
parameter_meta {
presetOption: {description: "This option applies multiple options at the same time.", category: "required"}
sort: {description: "Sort the output bam file.", category: "advanced"}
sample: {description: "Name of the sample"}
referenceMMI: {description: "MMI file for the reference.", category: "required"}
queryFile: {description: "BAM file with reads to align against the reference.", category: "required"}
cores: {description: "The number of cores to be used.", category: "advanced"}
memory: {description: "The amount of memory available to the job.", category: "advanced"}
timeMinutes: {description: "The maximum amount of time the job will run in minutes.", category: "advanced"}
dockerImage: {description: "The docker image used for this task. Changing this may result in errors which the developers may choose not to address.", category: "advanced"}
# output
outputAlignmentFile: {description: "Mapped bam files."}
}
}
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