Skip to content
Snippets Groups Projects
  • António Paulo's avatar
    48f0b3cf
    Add flags to varDict filtering script "var2vcf_paired.pl" · 48f0b3cf
    António Paulo authored
    Flag description:
      -M  If set, output only candidate somatic
      -A  Indicate to output all variants at the same position.  By default, only the variant with the highest allele frequency is converted to VCF
      -Q  float The minimum mapping quality.  Defaults to 0 for Illumina sequencing => Using -Q 20 as default
      -d  integer The minimum total depth.  Defaults to 5 => Using -d 8 as default
      -v  integer The minimum variant depth.  Defaults to 3 => Using -v 4 as default
      -f  float The minimum allele frequency.  Defaults to 0.02
    
    Note that "-M" is a soft filter (see https://github.com/AstraZeneca-NGS/VarDictJava/issues/247). This means that there will still be variants with "STATUS=Germline" with a "PASS" filter.
    48f0b3cf
    History
    Add flags to varDict filtering script "var2vcf_paired.pl"
    António Paulo authored
    Flag description:
      -M  If set, output only candidate somatic
      -A  Indicate to output all variants at the same position.  By default, only the variant with the highest allele frequency is converted to VCF
      -Q  float The minimum mapping quality.  Defaults to 0 for Illumina sequencing => Using -Q 20 as default
      -d  integer The minimum total depth.  Defaults to 5 => Using -d 8 as default
      -v  integer The minimum variant depth.  Defaults to 3 => Using -v 4 as default
      -f  float The minimum allele frequency.  Defaults to 0.02
    
    Note that "-M" is a soft filter (see https://github.com/AstraZeneca-NGS/VarDictJava/issues/247). This means that there will still be variants with "STATUS=Germline" with a "PASS" filter.