> **PCR Report** <br> [:arrow_down:]() PDF file, containing a report on fragment analysis of the PCR products. <br> <br>
> **Sanger Sequences** <br> [:arrow_down:]() FASTA file, containing Sanger sequences based on the PCR experiment.
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**Single-molecule RNA FISH** relies on the combined fluorescence from 25-48 singly fluorophore labeled oligonucleotides bound to the same RNA. By using the fluorescence from a guide probe set in one dye, the fluorescence from one or more exon-specific probe sets with <25oligonucleotides,andeachlabeledwithaseparatedye,canbeaccuratelyregisteredasbelongtothesameRNA.Theoptimalnumberofoligonucleotidesperspecificsetmustbeexperimentallydetermined.Herewecoulduseprobesetswithonly9oligonucleotides.<br>