From dbff8c0736f70d03c3a9c1aebab578b4e056d734 Mon Sep 17 00:00:00 2001
From: Sander van der Zeeuw <s.a.j.van_der_zeeuw@lumc.nl>
Date: Thu, 29 Jan 2015 10:25:45 +0100
Subject: [PATCH] bug fixes for macs2 wrapper
---
.../extensions/macs2/Macs2CallPeak.scala | 62 +++++++++++--------
1 file changed, 37 insertions(+), 25 deletions(-)
diff --git a/public/biopet-framework/src/main/scala/nl/lumc/sasc/biopet/extensions/macs2/Macs2CallPeak.scala b/public/biopet-framework/src/main/scala/nl/lumc/sasc/biopet/extensions/macs2/Macs2CallPeak.scala
index 40fc868e9..0722e99a2 100644
--- a/public/biopet-framework/src/main/scala/nl/lumc/sasc/biopet/extensions/macs2/Macs2CallPeak.scala
+++ b/public/biopet-framework/src/main/scala/nl/lumc/sasc/biopet/extensions/macs2/Macs2CallPeak.scala
@@ -7,70 +7,82 @@ import org.broadinstitute.gatk.utils.commandline.{ Output, Input }
class Macs2CallPeak(val root: Configurable) extends Macs2 {
@Input(doc = "Treatment input", required = true)
- var treatment: List[File] = Nil
+ var treatment: File = _
@Input(doc = "Control input", required = false)
- var control: List[File] = Nil
+ var control: File = _
@Output(doc = "Output file NARROWPEAKS")
- var output_narrow: File = _
+ private var output_narrow: File = _
@Output(doc = "Output file BROADPEAKS")
- var output_broad: File = _
+ private var output_broad: File = _
@Output(doc = "Output in Excel format")
- var output_xls: File = _
+ private var output_xls: File = _
@Output(doc = "R script with Bimodal model")
- var output_r: File = _
+ private var output_r: File = _
@Output(doc = "Output file Bedgraph")
- var output_bdg: File = _
+ private var output_bdg: File = _
+
+ @Output(doc = "Output file gappedPeak")
+ private var output_gapped: File = _
var fileformat: String = config("fileformat")
var gsize: Option[Float] = config("gsize")
- var keepdup: Boolean = config("keep-dup")
+ var keepdup: Boolean = config("keep-dup", default = false)
var buffersize: Option[Int] = config("buffer-size")
var outputdir: String = config("outputDir")
var name: String = config("name")
- var bdg: Boolean = config("bdg")
- var verbose: Boolean = config("verbose")
+ var bdg: Boolean = config("bdg", default = false)
+ var verbose: Boolean = config("verbose", default = false)
var tsize: Option[Int] = config("tsize")
var bandwith: Option[Int] = config("bandwith")
var mfold: Option[Float] = config("mfold")
- var fixbimodel: Boolean = config("fixbimodel")
- var nomodel: Boolean = config("nomodel")
+ var fixbimodel: Boolean = config("fixbimodel", default = false)
+ var nomodel: Boolean = config("nomodel", default = false)
var shift: Option[Int] = config("shift")
var qvalue: Option[Float] = config("qvalue")
var pvalue: Option[Float] = config("pvalue")
- var tolarge: Boolean = config("tolarge")
- var downsample: Boolean = config("downsample")
- var nolambda: Boolean = config("nolambda")
+ var tolarge: Boolean = config("tolarge", default = false)
+ var downsample: Boolean = config("downsample", default = false)
+ var nolambda: Boolean = config("nolambda", default = false)
var slocal: Option[Int] = config("slocal")
var llocal: Option[Int] = config("llocal")
- var broad: Boolean = config("broad")
+ var broad: Boolean = config("broad", default = false)
var broadcutoff: Option[Int] = config("broadcutoff")
- var callsummits: Boolean = config("callsummits")
+ var callsummits: Boolean = config("callsummits", default = false)
+
+ override def afterGraph: Unit = {
+ output_narrow = new File(outputdir + name + ".narrowPeak")
+ output_broad = new File(outputdir + name + ".broadPeak")
+ output_xls = new File(outputdir + name + ".xls")
+ output_bdg = new File(outputdir + name + ".bdg")
+ output_r = new File(outputdir + name + ".r")
+ output_gapped = new File(outputdir + name + ".gappedPeak")
+ }
def cmdLine = {
required(executable) + required("callpeak") +
- repeat("--treatment", treatment) + /* Treatment sample */
- repeat("--control", control) + /* Control sample */
+ required("--treatment", treatment) + /* Treatment sample */
+ optional("--control", control) + /* Control sample */
optional("-f", fileformat) + /* Input file format */
required("-g", gsize) + /* Estimated genome size.(format: 2.7e9) (presets: hs, mm, ce, dm) */
conditional(keepdup, "--keep-dup") + /* Whether to keep duplicates */
optional("--buffer-size", buffersize) + /* Buffer size */
- required("--outputDir", outputdir) + /* The output directory */
- optional("-n", name) + /* prefix name of the output files. (note that also the peak names inside the files will have this name */
+ required("--outdir", outputdir) + /* The output directory */
+ optional("--name", name) + /* prefix name of the output files. (note that also the peak names inside the files will have this name */
conditional(bdg, "-B") + /* Whether to output in BDG format */
conditional(verbose, "--verbose") + /* Whether to output verbosely */
optional("--tsize", tsize) + /* Sets custom tag length, if not specified macs will use first 10 sequences to estimate the size */
optional("--bw", bandwith) + /* The bandwith to use for model building. Set this parameter as the sonication fragment size estimated in the wetlab */
optional("--mfold", mfold) + /* The parameter to select regions within the model fold. Must be a upper and lower limit. */
- conditional(fixbimodel, "--fix-bimodal") /* Whether turn on the auto paired-peak model process. If it's set, when MACS failed to build paired model, it will use the nomodel settings, the '--extsize' parameter to extend each tags. If set, MACS will be terminated if paried-peak model is failed. */
- conditional(nomodel, "--nomodel") /* While on, MACS will bypass building the shifting model */
- optional("--shift", shift) /* You can set an arbitrary shift in basepairs here */
- optional("--extsize", shift) + /* While '--nomodel' is set, MACS uses this parameter to extend reads in 5'->3' direction to fix-sized fragments. For example, if the size of binding region for your transcription factor is 200 bp, and you want to bypass the model building by MACS, this parameter can be set as 200. This option is only valid when --nomodel is set or when MACS fails to build model and --fix-bimodal is on. */
+ conditional(fixbimodel, "--fix-bimodal") + /* Whether turn on the auto paired-peak model process. If it's set, when MACS failed to build paired model, it will use the nomodel settings, the '--extsize' parameter to extend each tags. If set, MACS will be terminated if paried-peak model is failed. */
+ conditional(nomodel, "--nomodel") + /* While on, MACS will bypass building the shifting model */
+ optional("--shift", shift) + /* You can set an arbitrary shift in basepairs here */
+ optional("--extsize", shift) + /* While '--nomodel' is set, MACS uses this parameter to extend reads in 5'->3' direction to fix-sized fragments. For example, if the size of binding region for your transcription factor is 200 bp, and you want to bypass the model building by MACS, this parameter can be set as 200. This option is only valid when --nomodel is set or when MACS fails to build model and --fix-bimodal is on. */
optional("--qvalue", qvalue) + /* the Q-value(FDR) cutoff */
optional("--pvalue", pvalue) + /* The P-value cutoff, if --pvalue is set no Qvalue is calculated */
conditional(tolarge, "--to-large") + /* Whether to scale up the smallest input file to the larger one */
--
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