diff --git a/biopet-framework/src/test/resources/single05.bam b/biopet-framework/src/test/resources/single05.bam
new file mode 100644
index 0000000000000000000000000000000000000000..e9fab38e6a945bf942921353d4a443cee881de2e
Binary files /dev/null and b/biopet-framework/src/test/resources/single05.bam differ
diff --git a/biopet-framework/src/test/resources/single05.bam.bai b/biopet-framework/src/test/resources/single05.bam.bai
new file mode 100644
index 0000000000000000000000000000000000000000..7a081f4700900bd7f3da6634d39b676b5547130a
Binary files /dev/null and b/biopet-framework/src/test/resources/single05.bam.bai differ
diff --git a/biopet-framework/src/test/resources/single05.sam b/biopet-framework/src/test/resources/single05.sam
new file mode 100644
index 0000000000000000000000000000000000000000..b5fe7ceae436ea67e0a7eb44b5f2b7b051cf72bf
--- /dev/null
+++ b/biopet-framework/src/test/resources/single05.sam
@@ -0,0 +1,9 @@
+@HD	VN:1.0	SO:coordinate
+@SQ	SN:chrQ	LN:10000
+@SQ	SN:chrR	LN:10000
+@RG	ID:001	DS:single-end reads	SM:WipeReadsTestCase
+r02	16	chrQ	50	60	10M	*	0	0	TACGTACGTA	EEFFGGHHII	RG:Z:001
+r04	0	chrQ	500	60	10M	*	0	0	CGTACGTACG	EEFFGGHHII	RG:Z:001
+r01	0	chrR	50	60	10M	*	0	0	TACGTACGTA	EEFFGGHHII	RG:Z:001
+r03	16	chrR	500	60	10M	*	0	0	GGGGGAAAAA	GGGGGGGGGG	RG:Z:001
+r05	4	*	0	0	*	*	0	0	ATATATATAT	HIHIHIHIHI	RG:Z:001
diff --git a/biopet-framework/src/test/scala/nl/lumc/sasc/biopet/tools/WipeReadsUnitTest.scala b/biopet-framework/src/test/scala/nl/lumc/sasc/biopet/tools/WipeReadsUnitTest.scala
index 772ed62950b4d08074ce535db339a4865fb69db3..f05b34219fa9494c5cbf2778c733bd5b170b46d9 100644
--- a/biopet-framework/src/test/scala/nl/lumc/sasc/biopet/tools/WipeReadsUnitTest.scala
+++ b/biopet-framework/src/test/scala/nl/lumc/sasc/biopet/tools/WipeReadsUnitTest.scala
@@ -35,6 +35,7 @@ class WipeReadsUnitTest extends TestNGSuite with MockitoSugar with Matchers {
   private val samP: SAMLineParser = {
     val samh = new SAMFileHeader
     samh.addSequence(new SAMSequenceRecord("chrQ", 10000))
+    samh.addSequence(new SAMSequenceRecord("chrR", 10000))
     samh.addReadGroup(new SAMReadGroupRecord("001"))
     samh.addReadGroup(new SAMReadGroupRecord("002"))
     new SAMLineParser(samh)
@@ -83,6 +84,15 @@ class WipeReadsUnitTest extends TestNGSuite with MockitoSugar with Matchers {
   val sBamFile3 = new File(resourcePath("/single03.bam"))
   val sBamFile4 = new File(resourcePath("/single04.bam"))
 
+  val sBamFile5 = new File(resourcePath("/single05.bam"))
+  val sBamRecs5 = makeSams(
+    "r02\t16\tchrR\t50\t60\t10M\t*\t0\t0\tTACGTACGTA\tEEFFGGHHII\tRG:Z:001",
+    "r04\t0\tchrQ\t500\t60\t10M\t*\t0\t0\tCGTACGTACG\tEEFFGGHHII\tRG:Z:001",
+    "r01\t0\tchrR\t50\t60\t10M\t*\t0\t0\tTACGTACGTA\tEEFFGGHHII\tRG:Z:001",
+    "r03\t16\tchrQ\t500\t60\t10M\t*\t0\t0\tGGGGGAAAAA\tGGGGGGGGGG\tRG:Z:001",
+    "r05\t4\t*\t0\t0\t*\t*\t0\t0\tATATATATAT\tHIHIHIHIHI\tRG:Z:001"
+  )
+
   val pBamFile1 = new File(resourcePath("/paired01.bam"))
   val pBamRecs1 = makeSams(
     "r02\t99\tchrQ\t50\t60\t10M\t=\t90\t50\tTACGTACGTA\tEEFFGGHHII\tRG:Z:001",
@@ -196,6 +206,19 @@ class WipeReadsUnitTest extends TestNGSuite with MockitoSugar with Matchers {
     filterNotFunc(sBamRecs1(6)) shouldBe false
   }
 
+  @Test def testSingleBamDifferentChromosomes() = {
+    val intervals: List[Interval] = List(
+      new Interval("chrQ", 50, 55),
+      new Interval("chrR", 500, 505)
+    )
+    val filterNotFunc = makeFilterNotFunction(intervals, sBamFile5, bloomSize = bloomSize, bloomFp = bloomFp)
+    filterNotFunc(sBamRecs5(0)) shouldBe true
+    filterNotFunc(sBamRecs5(1)) shouldBe false
+    filterNotFunc(sBamRecs5(2)) shouldBe false
+    filterNotFunc(sBamRecs5(3)) shouldBe true
+    filterNotFunc(sBamRecs5(4)) shouldBe false
+  }
+
   @Test def testSingleBamFilterOutMultiNotSet() = {
     val intervals: List[Interval] = List(
       new Interval("chrQ", 291, 320), // overlaps r01, second hit,