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biopet.biopet
Commits
55fc13c4
Commit
55fc13c4
authored
Mar 16, 2015
by
Peter van 't Hof
Browse files
Add documentation for ShivaVariantcalling
parent
36ddbb2a
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1
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docs/pipelines/shiva.md
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55fc13c4
...
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@@ -24,6 +24,10 @@ The pipeline accepts ```.fastq & .bam``` files as input.
Note that one should first create the appropriate
[
configs
](
../general/config.md
)
.
### Full pipeline
The full pipeline can start from fastq or from bam file. This pipeline will include pre process steps for the bam files.
To get the help menu:
~~~
java -jar </path/to/biopet.jar> pipeline shiva -h
...
...
@@ -37,11 +41,36 @@ Arguments for Shiva:
To run the pipeline:
~~~
java -jar </path/to/biopet.jar> pipeline shiva
-run
-config MySamples.json -config MySettings.json
java -jar </path/to/biopet.jar> pipeline shiva -config MySamples.json -config MySettings.json
-run
~~~
To perform a dry run simply remove
`-run`
from the commandline call.
### Just variantcalling
This will not do any pre process steps on the bam files.
To get the help menu:
~~~
java -jar </path/to/biopet.jar> pipeline shivavariantcalling -h
Arguments for ShivaVariantcalling:
-BAM,--inputbams <inputbams> Bam files (should be deduped bams)
-sample,--sampleid <sampleid> Sample ID (only effects summary and not required)
-library,--libid <libid> Library ID (only effects summary and not required)
-config,--config_file <config_file> JSON config file(s)
-DSC,--disablescatter Disable all scatters
~~~
To run the pipeline:
~~~
java -jar </path/to/biopet.jar> pipeline shivavariantcalling -config MySettings.json -run
~~~
To perform a dry run simply remove
`-run`
from the commandline call.
----
## Variantcaller
...
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@@ -100,6 +129,7 @@ To view all possible config options please navigate to our Gitlab wiki page
}
}
},
"reference"
:
"<location of fasta of reference>"
,
"variantcallers"
:
[
"haplotypecaller"
,
"unifiedgenotyper"
],
"output_dir"
:
"<output directory>"
}
...
...
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