Merge branch 'feature-generate_indexes' into 'develop'

Feature generate indexes

see also #187 

See merge request !211

public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/extensions/Cat.scala

@@ -31,12 +31,14 @@ class Cat(val root: Configurable) extends BiopetCommandLineFunction {
   @Output(doc = "Unzipped file", required = true)
   var output: File = _
 
+  var appending = false
+
   executable = config("exe", default = "cat")
 
   /** return commandline to execute */
   def cmdLine = required(executable) +
     (if (inputAsStdin) "" else repeat(input)) +
-    (if (outputAsStsout) "" else " > " + required(output))
+    (if (outputAsStsout) "" else (if (appending) " >> " else " > ") + required(output))
 }
 
 /**

public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/extensions/Curl.scala

+package nl.lumc.sasc.biopet.extensions
+
+import java.io.File
+
+import nl.lumc.sasc.biopet.core.{ Version, BiopetCommandLineFunction }
+import nl.lumc.sasc.biopet.utils.config.Configurable
+import org.broadinstitute.gatk.utils.commandline.Output
+
+/**
+ * Created by pjvan_thof on 8/11/15.
+ */
+class Curl(val root: Configurable) extends BiopetCommandLineFunction with Version {
+  @Output
+  var output: File = _
+
+  var url: String = _
+
+  executable = config("exe", default = "curl")
+  def versionCommand = executable + " --version"
+  def versionRegex = """curl (\w+\.\w+\.\w+) .*""".r
+
+  def cmdLine: String = required(executable) + required(url) + " > " + required(output)
+}

public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/extensions/Star.scala

@@ -62,7 +62,7 @@ class Star(val root: Configurable) extends BiopetCommandLineFunction with Refere
 
   var genomeDir: File = null
   var runmode: String = _
-  var sjdbOverhang: Int = _
+  var sjdbOverhang: Option[Int] = None
   var outFileNamePrefix: String = _
   var runThreadN: Option[Int] = config("runThreadN")
 
@@ -73,24 +73,24 @@ class Star(val root: Configurable) extends BiopetCommandLineFunction with Refere
   override def beforeGraph() {
     super.beforeGraph()
     if (reference == null) reference = referenceFasta()
-    genomeDir = config("genomeDir", new File(reference.getAbsoluteFile.getParent, "star"))
     if (outFileNamePrefix != null && !outFileNamePrefix.endsWith(".")) outFileNamePrefix += "."
     val prefix = if (outFileNamePrefix != null) outputDir + File.separator + outFileNamePrefix else outputDir + File.separator
     if (runmode == null) {
       outputSam = new File(prefix + "Aligned.out.sam")
       outputTab = new File(prefix + "SJ.out.tab")
+      genomeDir = config("genomeDir", new File(reference.getAbsoluteFile.getParent, "star"))
     } else if (runmode == "genomeGenerate") {
       genomeDir = outputDir
       outputGenome = new File(prefix + "Genome")
       outputSA = new File(prefix + "SA")
       outputSAindex = new File(prefix + "SAindex")
-      sjdbOverhang = config("sjdboverhang", 75)
+      sjdbOverhang = config("sjdboverhang")
     }
   }
 
   /** Returns command to execute */
   def cmdLine = {
-    var cmd: String = required("cd", outputDir) + "&&" + required(executable)
+    var cmd: String = required("cd", outputDir) + " && " + required(executable)
     if (runmode != null && runmode == "genomeGenerate") { // Create index
       cmd += required("--runMode", runmode) +
         required("--genomeFastaFiles", reference)
@@ -100,8 +100,8 @@ class Star(val root: Configurable) extends BiopetCommandLineFunction with Refere
     cmd += required("--genomeDir", genomeDir) +
       optional("--sjdbFileChrStartEnd", sjdbFileChrStartEnd) +
       optional("--runThreadN", threads) +
-      optional("--outFileNamePrefix", outFileNamePrefix)
-    if (sjdbOverhang > 0) cmd += optional("--sjdbOverhang", sjdbOverhang)
+      optional("--outFileNamePrefix", outFileNamePrefix) +
+      optional("--sjdbOverhang", sjdbOverhang)
 
     cmd
   }

public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/extensions/Tabix.scala

@@ -66,6 +66,7 @@ class Tabix(val root: Configurable) extends BiopetCommandLineFunction with Versi
   private val validFormats: Set[String] = Set("gff", "bed", "sam", "vcf", "psltbl")
 
   override def beforeGraph(): Unit = {
+    super.beforeGraph()
     p match {
       case Some(fmt) =>
         require(validFormats.contains(fmt), "-p flag must be one of " + validFormats.mkString(", "))

public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/extensions/TarExtract.scala

+package nl.lumc.sasc.biopet.extensions
+
+import java.io.File
+
+import nl.lumc.sasc.biopet.core.{ Version, BiopetCommandLineFunction }
+import nl.lumc.sasc.biopet.utils.config.Configurable
+import org.broadinstitute.gatk.utils.commandline.{ Argument, Input }
+
+/**
+ * Created by pjvan_thof on 8/11/15.
+ */
+class TarExtract(val root: Configurable) extends BiopetCommandLineFunction with Version {
+  @Input(required = true)
+  var inputTar: File = _
+
+  @Argument(required = true)
+  var outputDir: File = _
+
+  executable = config("exe", default = "tar", freeVar = false)
+  def versionCommand = executable + " --version"
+  def versionRegex = """tar \(GNU tar\) (.*)""".r
+
+  override def beforeGraph: Unit = {
+    super.beforeGraph
+    jobLocalDir = outputDir
+    jobOutputFile = new File(outputDir, "." + inputTar.getName + ".tar.out")
+  }
+
+  def cmdLine: String = required(executable) +
+    required("-x") +
+    required("-f", inputTar) +
+    required("--directory", outputDir)
+}

public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/extensions/Zcat.scala

@@ -29,6 +29,8 @@ class Zcat(val root: Configurable) extends BiopetCommandLineFunction with Versio
   @Output(doc = "Unzipped file", required = true)
   var output: File = _
 
+  var appending = false
+
   executable = config("exe", default = "zcat")
 
   def versionRegex = """zcat \(gzip\) (.*)""".r
@@ -37,7 +39,7 @@ class Zcat(val root: Configurable) extends BiopetCommandLineFunction with Versio
   /** Returns command to execute */
   def cmdLine = required(executable) +
     (if (inputAsStdin) "" else repeat(input)) +
-    (if (outputAsStsout) "" else " > " + required(output))
+    (if (outputAsStsout) "" else (if (appending) " >> " else " > ") + required(output))
 }
 
 object Zcat {

public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/extensions/Bowtie.scala → public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/extensions/bowtie/Bowtie.scala

@@ -13,7 +13,7 @@
  * license; For commercial users or users who do not want to follow the AGPL
  * license, please contact us to obtain a separate license.
  */
-package nl.lumc.sasc.biopet.extensions
+package nl.lumc.sasc.biopet.extensions.bowtie
 
 import java.io.File
 

public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/extensions/Bowtie2.scala → public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/extensions/bowtie/Bowtie2.scala

-package nl.lumc.sasc.biopet.extensions
+package nl.lumc.sasc.biopet.extensions.bowtie
 
 import java.io.File
 
-import nl.lumc.sasc.biopet.core.{ BiopetCommandLineFunction, Reference, Version }
+import nl.lumc.sasc.biopet.core.{BiopetCommandLineFunction, Reference, Version}
 import nl.lumc.sasc.biopet.utils.Logging
 import nl.lumc.sasc.biopet.utils.config.Configurable
-import org.broadinstitute.gatk.utils.commandline.{ Input, Output }
+import org.broadinstitute.gatk.utils.commandline.{Input, Output}
 
 /**
  * Extension for bowtie 2

public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/extensions/bowtie/Bowtie2Build.scala

+package nl.lumc.sasc.biopet.extensions.bowtie
+
+import java.io.File
+
+import nl.lumc.sasc.biopet.core.{ Version, BiopetCommandLineFunction }
+import nl.lumc.sasc.biopet.utils.config.Configurable
+import org.broadinstitute.gatk.utils.commandline.{ Argument, Input }
+
+/**
+ * Created by pjvan_thof on 8/15/15.
+ */
+class Bowtie2Build(val root: Configurable) extends BiopetCommandLineFunction with Version {
+  @Input(required = true)
+  var reference: File = _
+
+  @Argument(required = true)
+  var baseName: String = _
+
+  executable = config("exe", default = "bowtie2-build", freeVar = false)
+  def versionRegex = """.*[Vv]ersion:? (\d*\.\d*\.\d*)""".r
+  def versionCommand = executable + " --version"
+
+  override def defaultCoreMemory = 15.0
+
+  override def beforeGraph: Unit = {
+    outputFiles ::= new File(reference.getParentFile, baseName + ".1.bt2")
+    outputFiles ::= new File(reference.getParentFile, baseName + ".2.bt2")
+  }
+
+  def cmdLine = required("cd", reference.getParentFile) + " && " +
+    required(executable) +
+    required(reference) +
+    required(baseName)
+}

public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/extensions/bowtie/BowtieBuild.scala

+package nl.lumc.sasc.biopet.extensions.bowtie
+
+import java.io.File
+
+import nl.lumc.sasc.biopet.core.{ Version, BiopetCommandLineFunction }
+import nl.lumc.sasc.biopet.utils.config.Configurable
+import org.broadinstitute.gatk.utils.commandline.{ Argument, Input }
+
+/**
+ * Created by pjvan_thof on 8/15/15.
+ */
+class BowtieBuild(val root: Configurable) extends BiopetCommandLineFunction with Version {
+  @Input(required = true)
+  var reference: File = _
+
+  @Argument(required = true)
+  var baseName: String = _
+
+  executable = config("exe", default = "bowtie-build", freeVar = false)
+  def versionRegex = """.*[Vv]ersion:? (\d*\.\d*\.\d*)""".r
+  def versionCommand = executable + " --version"
+
+  override def defaultCoreMemory = 15.0
+
+  override def beforeGraph: Unit = {
+    outputFiles ::= new File(reference.getParentFile, baseName + ".1.ebwt")
+    outputFiles ::= new File(reference.getParentFile, baseName + ".2.ebwt")
+  }
+
+  def cmdLine = required("cd", reference.getParentFile) + " && " +
+    required(executable) +
+    required(reference) +
+    required(baseName)
+}

public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/extensions/bwa/BwaIndex.scala

+/**
+ * Biopet is built on top of GATK Queue for building bioinformatic
+ * pipelines. It is mainly intended to support LUMC SHARK cluster which is running
+ * SGE. But other types of HPC that are supported by GATK Queue (such as PBS)
+ * should also be able to execute Biopet tools and pipelines.
+ *
+ * Copyright 2014 Sequencing Analysis Support Core - Leiden University Medical Center
+ *
+ * Contact us at: sasc@lumc.nl
+ *
+ * A dual licensing mode is applied. The source code within this project that are
+ * not part of GATK Queue is freely available for non-commercial use under an AGPL
+ * license; For commercial users or users who do not want to follow the AGPL
+ * license, please contact us to obtain a separate license.
+ */
+package nl.lumc.sasc.biopet.extensions.bwa
+
+import java.io.File
+
+import nl.lumc.sasc.biopet.utils.config.Configurable
+import org.broadinstitute.gatk.utils.commandline.{ Input, Output }
+
+/**
+ * Extension for bwa aln
+ *
+ * Based on version 0.7.12-r1039
+ *
+ * Created by pjvan_thof on 1/16/15.
+ */
+class BwaIndex(val root: Configurable) extends Bwa {
+  @Input(doc = "Fastq file", required = true)
+  var reference: File = _
+
+  @Output(doc = "Index files for bwa", required = false)
+  private var output: List[File] = Nil
+
+  var a: Option[String] = config("a", freeVar = false)
+  var p: Option[String] = config("p", freeVar = false)
+  var b: Option[Int] = config("e", freeVar = false)
+  var _6: Boolean = config("6", default = false, freeVar = false)
+
+  override def defaultCoreMemory = 35.0
+
+  override def beforeGraph() {
+    super.beforeGraph()
+    List(".sa", ".pac")
+      .foreach(ext => output ::= new File(reference.getAbsolutePath + ext))
+    output = output.distinct
+  }
+
+  /** Returns command to execute */
+  def cmdLine = required(executable) +
+    required("index") +
+    optional("-a", a) +
+    optional("-p", p) +
+    optional("-b", b) +
+    conditional(_6, "-6") +
+    required(reference)
+}

public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/extensions/gmap/GmapBuild.scala

+/**
+ * Biopet is built on top of GATK Queue for building bioinformatic
+ * pipelines. It is mainly intended to support LUMC SHARK cluster which is running
+ * SGE. But other types of HPC that are supported by GATK Queue (such as PBS)
+ * should also be able to execute Biopet tools and pipelines.
+ *
+ * Copyright 2014 Sequencing Analysis Support Core - Leiden University Medical Center
+ *
+ * Contact us at: sasc@lumc.nl
+ *
+ * A dual licensing mode is applied. The source code within this project that are
+ * not part of GATK Queue is freely available for non-commercial use under an AGPL
+ * license; For commercial users or users who do not want to follow the AGPL
+ * license, please contact us to obtain a separate license.
+ */
+package nl.lumc.sasc.biopet.extensions.gmap
+
+import java.io.File
+
+import nl.lumc.sasc.biopet.core.{ Version, BiopetCommandLineFunction, Reference }
+import nl.lumc.sasc.biopet.utils.config.Configurable
+import org.broadinstitute.gatk.utils.commandline.Input
+
+/**
+ * Wrapper for the gsnap command line tool
+ * Written based on gsnap version 2014-05-15
+ */
+class GmapBuild(val root: Configurable) extends BiopetCommandLineFunction with Reference with Version {
+
+  /** default executable */
+  executable = config("exe", default = "gmap_build", freeVar = false)
+
+  /** input file */
+  @Input(doc = "Input fasta files", required = true) //var input: List[File] = _
+  var fastaFiles: List[File] = Nil
+
+  /** genome directory */
+  var dir: File = _
+
+  /** genome database */
+  var db: String = _
+
+  override def defaultCoreMemory = 25.0
+
+  def versionRegex = """.* version (.*)""".r
+  def versionCommand = executable
+  override def versionExitcode = List(0, 1, 255)
+
+  override def beforeGraph: Unit = {
+    super.beforeGraph
+    jobOutputFile = new File(dir, ".log.out")
+  }
+
+  def cmdLine = {
+    required(executable) +
+      required("--dir", dir) +
+      optional("--db", db) +
+      repeat(fastaFiles)
+  }
+}

public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/extensions/Gsnap.scala → public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/extensions/gmap/Gsnap.scala

@@ -13,7 +13,7 @@
  * license; For commercial users or users who do not want to follow the AGPL
  * license, please contact us to obtain a separate license.
  */
-package nl.lumc.sasc.biopet.extensions
+package nl.lumc.sasc.biopet.extensions.gmap
 
 import java.io.File
 

public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/extensions/picard/CreateSequenceDictionary.scala

+/**
+ * Biopet is built on top of GATK Queue for building bioinformatic
+ * pipelines. It is mainly intended to support LUMC SHARK cluster which is running
+ * SGE. But other types of HPC that are supported by GATK Queue (such as PBS)
+ * should also be able to execute Biopet tools and pipelines.
+ *
+ * Copyright 2014 Sequencing Analysis Support Core - Leiden University Medical Center
+ *
+ * Contact us at: sasc@lumc.nl
+ *
+ * A dual licensing mode is applied. The source code within this project that are
+ * not part of GATK Queue is freely available for non-commercial use under an AGPL
+ * license; For commercial users or users who do not want to follow the AGPL
+ * license, please contact us to obtain a separate license.
+ */
+package nl.lumc.sasc.biopet.extensions.picard
+
+import java.io.File
+
+import nl.lumc.sasc.biopet.utils.config.Configurable
+import org.broadinstitute.gatk.utils.commandline.{ Input, Output }
+
+class CreateSequenceDictionary(val root: Configurable) extends Picard {
+
+  javaMainClass = new picard.sam.CreateSequenceDictionary().getClass.getName
+
+  @Input(required = true)
+  var reference: File = _
+
+  @Output(required = true)
+  var output: File = _
+
+  var genomeAssembly: Option[String] = config("genomeAssembly")
+  var uri: Option[String] = config("uri")
+  var species: Option[String] = config("species")
+  var truncateAtWhiteSpace: Boolean = config("truncateAtWhiteSpace", default = false)
+  var numSequences: Option[Int] = config("numSequences")
+
+  override def cmdLine = super.cmdLine +
+    required("REFERENCE=", reference, spaceSeparated = false) +
+    required("OUTPUT=", output, spaceSeparated = false) +
+    optional("GENOME_ASSEMBLY=", genomeAssembly, spaceSeparated = false) +
+    optional("URI=", uri, spaceSeparated = false) +
+    optional("SPECIES=", species, spaceSeparated = false) +
+    conditional(truncateAtWhiteSpace, "TRUNCATE_NAMES_AT_WHITESPACE=true") +
+    optional("NUM_SEQUENCES=", numSequences, spaceSeparated = false)
+}

public/biopet-extensions/src/main/scala/nl/lumc/sasc/biopet/extensions/samtools/SamtoolsFaidx.scala

+/**
+ * Biopet is built on top of GATK Queue for building bioinformatic
+ * pipelines. It is mainly intended to support LUMC SHARK cluster which is running
+ * SGE. But other types of HPC that are supported by GATK Queue (such as PBS)
+ * should also be able to execute Biopet tools and pipelines.
+ *
+ * Copyright 2014 Sequencing Analysis Support Core - Leiden University Medical Center
+ *
+ * Contact us at: sasc@lumc.nl
+ *
+ * A dual licensing mode is applied. The source code within this project that are
+ * not part of GATK Queue is freely available for non-commercial use under an AGPL
+ * license; For commercial users or users who do not want to follow the AGPL
+ * license, please contact us to obtain a separate license.
+ */
+package nl.lumc.sasc.biopet.extensions.samtools
+
+import java.io.File
+
+import nl.lumc.sasc.biopet.utils.config.Configurable
+import org.broadinstitute.gatk.utils.commandline.{ Input, Output }
+
+/** Extension for samtools flagstat */
+class SamtoolsFaidx(val root: Configurable) extends Samtools {
+  @Input(doc = "Bam File")
+  var input: File = _
+
+  @Output(doc = "output File")
+  private var _output: File = _
+
+  def output = _output
+
+  override def beforeGraph: Unit = {
+    super.beforeGraph
+    _output = new File(input.getParentFile, input.getName + ".fai")
+  }
+
+  /** Returns command to execute */
+  def cmdLine = required(executable) + required("faidx") + required(input)
+}
+
+object SamtoolsFaidx {
+  def apply(root: Configurable, input: File): SamtoolsFaidx = {
+    val faidx = new SamtoolsFaidx(root)
+    faidx.input = input
+    faidx._output = new File(input.getParentFile, input.getName + ".fai")
+    faidx
+  }
+}
\ No newline at end of file

public/biopet-extensions/src/test/scala/nl/lumc/sasc/biopet/extensions/GsnapTest.scala

@@ -16,6 +16,7 @@
 package nl.lumc.sasc.biopet.extensions
 
 import nl.lumc.sasc.biopet.utils.config.Config
+import nl.lumc.sasc.biopet.extensions.gmap.Gsnap
 import org.scalatest.Matchers
 import org.scalatest.testng.TestNGSuite
 import org.testng.SkipException

public/generate-indexes/pom.xml

+<?xml version="1.0" encoding="UTF-8"?>
+<!--
+
+    Biopet is built on top of GATK Queue for building bioinformatic
+    pipelines. It is mainly intended to support LUMC SHARK cluster which is running
+    SGE. But other types of HPC that are supported by GATK Queue (such as PBS)
+    should also be able to execute Biopet tools and pipelines.
+
+    Copyright 2014 Sequencing Analysis Support Core - Leiden University Medical Center
+
+    Contact us at: sasc@lumc.nl
+
+    A dual licensing mode is applied. The source code within this project that are
+    not part of GATK Queue is freely available for non-commercial use under an AGPL
+    license; For commercial users or users who do not want to follow the AGPL
+    license, please contact us to obtain a separate license.
+
+-->
+<project xmlns="http://maven.apache.org/POM/4.0.0"
+         xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
+         xsi:schemaLocation="http://maven.apache.org/POM/4.0.0 http://maven.apache.org/xsd/maven-4.0.0.xsd">
+    <modelVersion>4.0.0</modelVersion>
+
+    <artifactId>GenerateIndexes</artifactId>
+    <packaging>jar</packaging>
+
+    <parent>
+        <groupId>nl.lumc.sasc</groupId>
+        <artifactId>Biopet</artifactId>
+        <version>0.6.0-SNAPSHOT</version>
+        <relativePath>../</relativePath>
+    </parent>
+
+    <inceptionYear>2014</inceptionYear>
+    <name>GenerateIndexes</name>
+
+    <dependencies>
+        <dependency>
+            <groupId>nl.lumc.sasc</groupId>
+            <artifactId>BiopetCore</artifactId>
+            <version>${project.version}</version>
+        </dependency>
+        <dependency>
+            <groupId>nl.lumc.sasc</groupId>
+            <artifactId>BiopetExtensions</artifactId>
+            <version>${project.version}</version>
+        </dependency>
+    </dependencies>
+
+</project>
\ No newline at end of file

public/generate-indexes/src/main/scala/nl/lumc/sasc/biopet/pipelines/GenerateIndexes.scala

+/**
+ * Biopet is built on top of GATK Queue for building bioinformatic
+ * pipelines. It is mainly intended to support LUMC SHARK cluster which is running
+ * SGE. But other types of HPC that are supported by GATK Queue (such as PBS)
+ * should also be able to execute Biopet tools and pipelines.
+ *
+ * Copyright 2014 Sequencing Analysis Support Core - Leiden University Medical Center
+ *
+ * Contact us at: sasc@lumc.nl
+ *
+ * A dual licensing mode is applied. The source code within this project that are
+ * not part of GATK Queue is freely available for non-commercial use under an AGPL
+ * license; For commercial users or users who do not want to follow the AGPL
+ * license, please contact us to obtain a separate license.
+ */
+package nl.lumc.sasc.biopet.pipelines
+
+import java.io.PrintWriter
+import java.util
+
+import nl.lumc.sasc.biopet.core.extensions.Md5sum
+import nl.lumc.sasc.biopet.utils.config.Configurable
+import nl.lumc.sasc.biopet.core.{ BiopetCommandLineFunction, BiopetQScript, PipelineCommand }
+import nl.lumc.sasc.biopet.extensions._
+import nl.lumc.sasc.biopet.extensions.bowtie.{ Bowtie2Build, BowtieBuild }
+import nl.lumc.sasc.biopet.extensions.bwa.BwaIndex
+import nl.lumc.sasc.biopet.extensions.gatk.CombineVariants
+import nl.lumc.sasc.biopet.extensions.gmap.GmapBuild