Merge branch 'develop' into fix-bedtools_coverage_sorted

Conflicts:
	bammetrics/src/main/scala/nl/lumc/sasc/biopet/pipelines/bammetrics/BamMetrics.scala

bammetrics/src/main/scala/nl/lumc/sasc/biopet/pipelines/bammetrics/BamMetrics.scala

@@ -17,15 +17,19 @@ package nl.lumc.sasc.biopet.pipelines.bammetrics
 
 import java.io.File
 
+import nl.lumc.sasc.biopet.core.annotations.{ AnnotationRefFlat, RibosomalRefFlat }
 import nl.lumc.sasc.biopet.core.annotations.{AnnotationRefFlat, RibosomalRefFlat}
 import nl.lumc.sasc.biopet.utils.config.Configurable
 import nl.lumc.sasc.biopet.core.summary.SummaryQScript
+import nl.lumc.sasc.biopet.core.{ BiopetFifoPipe, PipelineCommand, Reference, SampleLibraryTag }
+import nl.lumc.sasc.biopet.extensions.bedtools.{ BedtoolsCoverage, BedtoolsIntersect }
 import nl.lumc.sasc.biopet.core.{BiopetFifoPipe, PipelineCommand, Reference, SampleLibraryTag}
 import nl.lumc.sasc.biopet.extensions.bedtools.{BedtoolsCoverage, BedtoolsIntersect, BedtoolsSort}
 import nl.lumc.sasc.biopet.extensions.picard._
 import nl.lumc.sasc.biopet.extensions.samtools.SamtoolsFlagstat
 import nl.lumc.sasc.biopet.pipelines.bammetrics.scripts.CoverageStats
 import nl.lumc.sasc.biopet.extensions.tools.BiopetFlagstat
+import nl.lumc.sasc.biopet.utils.intervals.BedCheck
 import org.broadinstitute.gatk.queue.QScript
 
 class BamMetrics(val root: Configurable) extends QScript
@@ -74,6 +78,8 @@ class BamMetrics(val root: Configurable) extends QScript
   /** executed before script */
   def init(): Unit = {
     inputFiles :+= new InputFile(inputBam)
+    ampliconBedFile.foreach(BedCheck.checkBedFileToReference(_, referenceFasta(), biopetError = true))
+    roiBedFiles.foreach(BedCheck.checkBedFileToReference(_, referenceFasta(), biopetError = true))
   }
 
   /** Script to add jobs */

bammetrics/src/test/scala/nl/lumc/sasc/biopet/pipelines/bammetrics/BamMetricsTest.scala

@@ -61,9 +61,9 @@ class BamMetricsTest extends TestNGSuite with Matchers {
   def testBamMetrics(rois: Int, amplicon: Boolean, rna: Boolean, wgs: Boolean) = {
     val map = ConfigUtils.mergeMaps(Map("output_dir" -> BamMetricsTest.outputDir, "rna_metrics" -> rna, "wgs_metrics" -> wgs),
       Map(BamMetricsTest.executables.toSeq: _*)) ++
-      (if (amplicon) Map("amplicon_bed" -> "amplicon.bed") else Map()) ++
+      (if (amplicon) Map("amplicon_bed" -> BamMetricsTest.ampliconBed.getAbsolutePath) else Map()) ++
       (if (rna) Map("annotation_refflat" -> "transcripts.refFlat") else Map()) ++
-      Map("regions_of_interest" -> (1 to rois).map("roi_" + _ + ".bed").toList)
+      Map("regions_of_interest" -> (1 to rois).map(BamMetricsTest.roi(_).getAbsolutePath).toList)
     val bammetrics: BamMetrics = initPipeline(map)
 
     bammetrics.inputBam = BamMetricsTest.bam
@@ -94,6 +94,14 @@ object BamMetricsTest {
 
   val bam = new File(outputDir, "input" + File.separator + "bla.bam")
   Files.touch(bam)
+  val ampliconBed = new File(outputDir, "input" + File.separator + "amplicon_bed.bed")
+  Files.touch(ampliconBed)
+
+  def roi(i: Int): File = {
+    val roi = new File(outputDir, "input" + File.separator + s"roi${i}.bed")
+    Files.touch(roi)
+    roi
+  }
 
   private def copyFile(name: String): Unit = {
     val is = getClass.getResourceAsStream("/" + name)

basty/src/main/scala/nl/lumc/sasc/biopet/pipelines/basty/Basty.scala

@@ -26,7 +26,6 @@ import nl.lumc.sasc.biopet.core.{ MultiSampleQScript, PipelineCommand }
 import nl.lumc.sasc.biopet.extensions.{ Cat, Raxml, RunGubbins }
 import nl.lumc.sasc.biopet.pipelines.shiva.Shiva
 import nl.lumc.sasc.biopet.extensions.tools.BastyGenerateFasta
-import nl.lumc.sasc.biopet.utils.ConfigUtils
 import nl.lumc.sasc.biopet.utils.config.Configurable
 import org.broadinstitute.gatk.queue.QScript
 

biopet-aggregate/pom.xml

@@ -17,6 +17,7 @@
 
     <modules>
         <module>../biopet-core</module>
+        <module>../generate-indexes</module>
         <module>../biopet-package</module>
         <module>../bammetrics</module>
         <module>../flexiprep</module>

biopet-core/src/main/scala/nl/lumc/sasc/biopet/core/BiopetFifoPipe.scala

@@ -73,6 +73,8 @@ class BiopetFifoPipe(val root: Configurable,
 
     _pipesJobs :::= commands
     _pipesJobs = _pipesJobs.distinct
+
+    analysisName = commands.map(_.analysisName).mkString("_")
   }
 
   override def beforeCmd(): Unit = {

biopet-core/src/main/scala/nl/lumc/sasc/biopet/core/BiopetPipe.scala

@@ -27,7 +27,7 @@ import org.broadinstitute.gatk.utils.commandline.{ Input, Output }
  */
 class BiopetPipe(val commands: List[BiopetCommandLineFunction]) extends BiopetCommandLineFunction {
 
-  @Input
+  @Input(required = false)
   lazy val input: List[File] = try {
     commands.flatMap(_.inputs)
   } catch {

biopet-core/src/main/scala/nl/lumc/sasc/biopet/core/report/ReportBuilder.scala

@@ -132,7 +132,7 @@ trait ReportBuilder extends ToolCommand {
     logger.info("Start")
 
     val argsParser = new OptParser
-    val cmdArgs: Args = argsParser.parse(args, Args()) getOrElse sys.exit(1)
+    val cmdArgs: Args = argsParser.parse(args, Args()) getOrElse (throw new IllegalArgumentException)
 
     require(cmdArgs.outputDir.exists(), "Output dir does not exist")
     require(cmdArgs.outputDir.isDirectory, "Output dir is not a directory")
@@ -205,7 +205,7 @@ trait ReportBuilder extends ToolCommand {
 
     val pageOutputDir = new File(outputDir, path.mkString(File.separator))
     pageOutputDir.mkdirs()
-    val rootPath = "./" + Array.fill(path.size)("src/main").mkString("")
+    val rootPath = "./" + Array.fill(path.size)("../").mkString
     val pageArgs = args ++ page.args ++
       Map("page" -> page,
         "path" -> path,

biopet-extensions/src/main/resources/nl/lumc/sasc/biopet/extensions/breakdancer/breakdancer2vcf.py

@@ -30,19 +30,21 @@ import csv
 import datetime
 
 
-def main(tsvfile, vcffile):
+def main(tsvfile, vcffile, samplename):
     '''
     :param tsvfile: filename of input file.tsv
     :type tsvfile: string
     :param vcffile: filename of output file.vcf
     :type vcffile: string
+    :param samplename: Name of the sample
+    :type samplename: string
     '''
     with open(tsvfile) as reader:
         # Parse file
         dictreader = _parse_tsvfile(reader)
 
         # Write out file
-        _format_vcffile(dictreader, vcffile)
+        _format_vcffile(dictreader, vcffile, samplename)
 
 def _parse_tsvfile(readable):
     '''
@@ -92,11 +94,11 @@ _tsv_fields = ('Chr1', 'Pos1', 'Orientation1',
 
 
 
-_vcf_fields = ('CHROM', 'POS', 'ID', 'REF', 'ALT', 'QUAL', 'FILTER', 'INFO', 'FORMAT', 'default')
+_vcf_fields = ['CHROM', 'POS', 'ID', 'REF', 'ALT', 'QUAL', 'FILTER', 'INFO', 'FORMAT']
 
 TS_NOW = datetime.datetime.now()
 
-VCF_HEADER = """##fileformat=VCFv4.1
+VCF_HEADER = """##fileformat=VCFv4.2
 ##fileDate={filedate}
 ##source=breakdancer-max
 ##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
@@ -106,6 +108,7 @@ VCF_HEADER = """##fileformat=VCFv4.1
 ##INFO=<ID=NOVEL,Number=0,Type=Flag,Description="Indicates a novel structural variation">
 ##INFO=<ID=SVEND,Number=1,Type=Integer,Description="End position of the variant described in this record">
 ##INFO=<ID=END,Number=1,Type=Integer,Description="End position of the variant described in this record">
+##INFO=<ID=SVMETHOD,Number=0,Type=String,Description="Program called with">
 ##INFO=<ID=SVTYPE,Number=1,Type=String,Description="Type of structural variant">
 ##INFO=<ID=SVLEN,Number=.,Type=Integer,Description="Difference in length between REF and ALT alleles">
 ##INFO=<ID=CIPOS,Number=2,Type=Integer,Description="Confidence interval around POS for imprecise variants">
@@ -138,7 +141,7 @@ VCF_HEADER = """##fileformat=VCFv4.1
 ##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
 ##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">""".format( filedate=TS_NOW.strftime( "%Y%m%d" ) )
 
-def _format_vcffile(dictreader, vcffile):
+def _format_vcffile(dictreader, vcffile, samplename):
     '''
     Create a pseudo .vcf file based on values read from DictReader instance.
     :param dictreader: DictReader instance to read data from
@@ -148,22 +151,22 @@ def _format_vcffile(dictreader, vcffile):
     '''
     FORMAT = "GT:DP"
     with open(vcffile, mode='w') as writer:
-        writer.write('{header}\n#{columns}\n'.format(header=VCF_HEADER, columns='\t'.join(_vcf_fields)))
+        writer.write('{header}\n#{columns}\n'.format(header=VCF_HEADER, columns='\t'.join(_vcf_fields + [samplename])))
         output_vcf = []
         for line in dictreader:
             CHROM = line['Chr1']
             # TODO Figure out whether we have zero or one based positioning
             POS = int(line['Pos1'])
-            ALT = '.'
+            ALT = '<{}>'.format(line['Type'])
             SVEND = int(line['Pos2'])
 
-            INFO = 'PROGRAM=breakdancer;SVTYPE={}'.format(line['Type'])
+            INFO = 'SVMETHOD=breakdancer;SVTYPE={}'.format(line['Type'])
 
             if line['Type'] not in ['CTX']:
                 INFO += ';SVLEN={}'.format(int(line['Size']))
                 INFO += ";SVEND={}".format(SVEND)
                 INFO += ";END={}".format(SVEND)
-            
+
             # write alternate ALT field for Intrachromosomal translocations
             if line['Type'] in ['CTX']:
                 ALT = "N[{}:{}[".format(line['Chr2'], line['Pos2'])
@@ -172,7 +175,7 @@ def _format_vcffile(dictreader, vcffile):
 
             SAMPLEINFO = "{}:{}".format( '1/.', line['num_Reads'] )
             # Create record
-            output_vcf.append([CHROM, POS, '.', '.', ALT, '.', 'PASS', INFO, FORMAT, SAMPLEINFO])
+            output_vcf.append([CHROM, POS, '.', 'N', ALT, '.', 'PASS', INFO, FORMAT, SAMPLEINFO])
 
         # Sort all results
         output_vcf.sort()
@@ -184,9 +187,11 @@ def _format_vcffile(dictreader, vcffile):
 if __name__ == '__main__':
     parser = argparse.ArgumentParser()
     parser.add_argument('-i', '--breakdancertsv', dest='breakdancertsv', type=str,
-            help='Breakdancer TSV outputfile')
+                        help='Breakdancer TSV outputfile')
     parser.add_argument('-o', '--outputvcf', dest='outputvcf', type=str,
-            help='Output vcf to')
+                        help='Output vcf to')
+    parser.add_argument('-s', '--sample', dest='sample', type=str,
+                        help='sample name')
 
     args = parser.parse_args()
-    main(args.breakdancertsv, args.outputvcf)
+    main(args.breakdancertsv, args.outputvcf, args.sample)

biopet-extensions/src/main/resources/nl/lumc/sasc/biopet/extensions/cnmops.R

+#!/usr/bin/env Rscript
+suppressPackageStartupMessages(library('cn.mops'))
+suppressPackageStartupMessages(library('optparse'))
+
+# Script from  https://git.lumc.nl/lgtc-bioinformatics/gapss3/blob/master/src/CNV/makeCnmops.sh
+# modified to take arguments
+
+option_list <- list(
+    make_option(c("--rawoutput"), dest="rawoutput"),
+    make_option(c("--cnv"), dest="cnv"),
+    make_option(c("--cnr"), dest="cnr"),
+    make_option(c("--chr"), dest="chr"),
+    make_option(c("--threads"), dest="threads", default=8, type="integer")
+    )
+
+parser <- OptionParser(usage = "%prog [options] file", option_list=option_list)
+arguments = parse_args(parser, positional_arguments=TRUE)
+opt = arguments$options
+args = arguments$args
+
+chromosome <- opt$chr
+CNVoutput <- opt$cnv
+CNRoutput <- opt$cnr
+bamFile <- args
+
+BAMFiles <- c(bamFile)
+bamDataRanges <- getReadCountsFromBAM(BAMFiles, mode="paired", refSeqName=chromosome, WL=1000, parallel=opt$threads)
+
+write.table(as.data.frame( bamDataRanges ), quote = FALSE, opt$rawoutput, row.names=FALSE)
+
+res <- cn.mops(bamDataRanges)
+res <- calcIntegerCopyNumbers(res)
+
+write.table(as.data.frame(cnvs(res)), quote = FALSE, CNVoutput, row.names=FALSE)
+write.table(as.data.frame(cnvr(res)), quote = FALSE, CNRoutput, row.names=FALSE)
+
+
+ppi <- 300
+plot_margins <- c(3,4,1,2)+0.1
+label_positions <- c(2,0.5,0)
+
+dir.create(chromosome, showWarnings=FALSE, recursive=TRUE, mode="0744")
+
+# Plot chromosome per sample.
+for ( i in 1:length(BAMFiles)){
+  png(file=paste(chromosome,"/",chromosome,"-segplot-",i,".png", sep=""),
+  width = 16 * ppi, height = 10 * ppi,
+    res=ppi, bg = "white"
+  )
+    par(mfrow = c(1,1))
+    par(mar=plot_margins)
+    par(mgp=label_positions)
+  segplot(res,sampleIdx=i)
+  dev.off()
+}
+
+# Plot cnvr regions.
+for ( i in 1:nrow(as.data.frame(cnvr(res)))) {
+  png(file=paste(chromosome,"/",chromosome,"-cnv-",i,".png",sep=""),
+  width = 16 * ppi, height = 10 * ppi,
+    res=ppi, bg = "white")
+    par(mfrow = c(1,1))
+    par(mar=plot_margins)
+    par(mgp=label_positions)
+  plot(res,which=i,toFile=TRUE)
+  dev.off()
+}
+

biopet-extensions/src/main/resources/nl/lumc/sasc/biopet/extensions/cnmops_wes.R

+#!/usr/bin/env Rscript
+suppressPackageStartupMessages(library('cn.mops'))
+suppressPackageStartupMessages(library('optparse'))
+
+# Script from  https://git.lumc.nl/lgtc-bioinformatics/gapss3/blob/master/src/CNV/makeCnmops.sh
+# modified to take arguments
+
+option_list <- list(
+    make_option(c("--rawoutput"), dest="rawoutput"),
+    make_option(c("--cnv"), dest="cnv"),
+    make_option(c("--cnr"), dest="cnr"),
+    make_option(c("--chr"), dest="chr"),
+    make_option(c("--targetBed"), dest="targetBed"),
+    make_option(c("--threads"), dest="threads", default=8, type="integer")
+    )
+
+parser <- OptionParser(usage = "%prog [options] file", option_list=option_list)
+arguments = parse_args(parser, positional_arguments=TRUE)
+opt = arguments$options
+args = arguments$args
+
+chromosome <- opt$chr
+CNVoutput <- opt$cnv
+CNRoutput <- opt$cnr
+bamFile <- args
+
+BAMFiles <- c(bamFile)
+
+### WES Specific code
+segments <- read.table(opt$targetBed, sep="\t", as.is=TRUE)
+# filter the segments by the requested chromosome
+segments <- segments[ segments[,1] == chromosome, ]
+gr <- GRanges(segments[,1],IRanges(segments[,2],segments[,3]))
+### END WES Specific code
+
+bamDataRanges <- getSegmentReadCountsFromBAM(BAMFiles, GR=gr, mode="paired", parallel=opt$threads)
+
+write.table(as.data.frame( bamDataRanges ), quote = FALSE, opt$rawoutput, row.names=FALSE)
+
+res <- exomecn.mops(bamDataRanges)
+res <- calcIntegerCopyNumbers(res)
+
+write.table(as.data.frame(cnvs(res)), quote = FALSE, CNVoutput, row.names=FALSE)
+write.table(as.data.frame(cnvr(res)), quote = FALSE, CNRoutput, row.names=FALSE)
+
+
+ppi <- 300
+plot_margins <- c(3,4,1,2)+0.1
+label_positions <- c(2,0.5,0)
+
+dir.create(chromosome, showWarnings=FALSE, recursive=TRUE, mode="0744")
+
+# Plot chromosome per sample.
+for ( i in 1:length(BAMFiles)){
+  png(file=paste(chromosome,"/",chromosome,"-segplot-",i,".png", sep=""),
+  width = 16 * ppi, height = 10 * ppi,
+    res=ppi, bg = "white"
+  )
+    par(mfrow = c(1,1))
+    par(mar=plot_margins)
+    par(mgp=label_positions)
+  segplot(res,sampleIdx=i)
+  dev.off()
+}
+
+# Plot cnvr regions.
+for ( i in 1:nrow(as.data.frame(cnvr(res)))) {
+  png(file=paste(chromosome,"/",chromosome,"-cnv-",i,".png",sep=""),
+  width = 16 * ppi, height = 10 * ppi,
+    res=ppi, bg = "white")
+    par(mfrow = c(1,1))
+    par(mar=plot_margins)
+    par(mgp=label_positions)
+  plot(res,which=i,toFile=TRUE)
+  dev.off()
+}
+

biopet-extensions/src/main/resources/nl/lumc/sasc/biopet/extensions/freec/freec_BAFPlot.R

+library('optparse')
+# Script taken from  http://bioinfo-out.curie.fr/projects/freec/tutorial.html and modified for biopet
+
+option_list <- list(
+    make_option(c("-i", "--input"), dest="input"),
+    make_option(c("-o", "--output"), dest="output")
+    )
+
+parser <- OptionParser(usage = "%prog [options] file", option_list=option_list)
+opt = parse_args(parser)
+
+
+#
+# Load Data
+#
+
+dataTable <-read.table(opt$input, header=TRUE);
+BAF<-data.frame(dataTable)
+chromosomes <- levels(dataTable$Chromosome)
+ppi <- 300
+plot_margins <- c(3,4,1,2)+0.1
+label_positions <- c(2,0.5,0)
+
+
+png(filename = opt$output, width = 16 * ppi, height = 10 * ppi,
+    res=ppi, bg = "white")
+par(mfrow = c(6,4))
+par(mar=plot_margins)
+par(mgp=label_positions)
+
+
+for (i in chromosomes) {
+    tt <- which(BAF$Chromosome==i)
+    if (length(tt)>0){
+    lBAF <-BAF[tt,]
+    plot(lBAF$Position,
+        lBAF$BAF,
+        ylim = c(-0.1,1.1),
+        xlab = paste ("position, chr",i),
+        ylab = "BAF",
+        pch = ".",
+        col = colors()[1])
+
+    tt <- which(lBAF$A==0.5)
+    points(lBAF$Position[tt],lBAF$BAF[tt],pch = ".",col = colors()[92])
+    tt <- which(lBAF$A!=0.5 & lBAF$A>=0)
+    points(lBAF$Position[tt],lBAF$BAF[tt],pch = ".",col = colors()[62])
+    tt <- 1
+    pres <- 1
+
+    if (length(lBAF$A)>4) {
+        for (j in c(2:(length(lBAF$A)-pres-1))) {
+            if (lBAF$A[j]==lBAF$A[j+pres]) {
+                tt[length(tt)+1] <- j
+            }
+        }
+        points(lBAF$Position[tt],lBAF$A[tt],pch = ".",col = colors()[24],cex=4)
+        points(lBAF$Position[tt],lBAF$B[tt],pch = ".",col = colors()[24],cex=4)
+    }
+
+    tt <- 1
+    pres <- 1
+    if (length(lBAF$FittedA)>4) {
+        for (j in c(2:(length(lBAF$FittedA)-pres-1))) {
+            if (lBAF$FittedA[j]==lBAF$FittedA[j+pres]) {
+                tt[length(tt)+1] <- j
+            }
+        }
+        points(lBAF$Position[tt],lBAF$FittedA[tt],pch = ".",col = colors()[463],cex=4)
+        points(lBAF$Position[tt],lBAF$FittedB[tt],pch = ".",col = colors()[463],cex=4)
+    }
+
+   }
+
+}
+dev.off()

biopet-extensions/src/main/resources/nl/lumc/sasc/biopet/extensions/freec/freec_CNVPlot.R

+library('optparse')
+library('naturalsort')
+
+# Script taken from  http://bioinfo-out.curie.fr/projects/freec/tutorial.html and modified for biopet
+
+option_list <- list(
+    make_option(c("-m", "--mappability"), dest="mappability"),
+    make_option(c("-p", "--ploidy"), default=2, type="integer", dest="ploidy"),
+    make_option(c("-i", "--input"), dest="input"),
+    make_option(c("-o", "--output"), dest="output")
+    )
+
+parser <- OptionParser(usage = "%prog [options] file", option_list=option_list)
+opt = parse_args(parser)
+
+
+#
+# Load mappability track
+#
+
+mappabilityFile <- opt$mappability
+mappabilityTrack <- read.table(mappabilityFile, header=FALSE, col.names=c("chrom", "start", "end", "score"))
+
+mappabilityTrack$Start <- mappabilityTrack$start+1
+mappabilityTrack$Chromosome <- gsub("chr", "", mappabilityTrack$chrom)
+
+
+#
+# Load Data
+#
+
+
+dataTable <- read.table( opt$input , header=TRUE)
+input_ratio <- data.frame(dataTable)
+
+chromosomes <- naturalsort(levels(input_ratio$Chromosome))
+input_ratio$Chromosome <- factor(input_ratio$Chromosome, levels=chromosomes, ordered=T)
+
+sorted_ratio <- input_ratio[order(input_ratio$Chromosome),]
+ratio <- merge(sorted_ratio, mappabilityTrack, sort=TRUE)
+ratio <- ratio[order(ratio$Chromosome, ratio$Start),]
+
+
+ploidy <- opt$ploidy
+ppi <- 300
+plot_margins <- c(3,4,1,2)+0.1
+label_positions <- c(2,0.5,0)
+
+
+maxLevelToPlot <- 3
+for (i in c(1:length(ratio$Ratio))) {
+	if (ratio$Ratio[i]>maxLevelToPlot) {
+		ratio$Ratio[i]=maxLevelToPlot
+	}
+}
+
+#
+# Plot the graphs per chromosome
+#
+
+for (i in chromosomes) {
+
+    png(filename = paste(opt$output, ".", i,".png",sep=""), width = 4 * ppi, height = 2.5 * ppi,
+        res=ppi, bg = "white")
+    par(mfrow = c(1,1))
+    par(mar=plot_margins)
+    par(mgp=label_positions)
+
+
+    tt <- which(ratio$Chromosome==i)
+    if (length(tt)>0) {
+        plot(ratio$Start[tt],
+                ratio$Ratio[tt]*ploidy,
+                ylim = c(0,maxLevelToPlot*ploidy),
+                xlab = paste ("position, chr",i),
+                ylab = "normalized CN",
+                pch = ".",
+                col = colors()[88])
+
+        title(outer=TRUE)
+        tt <- which(ratio$Chromosome==i  & ratio$CopyNumber>ploidy )
+        points(ratio$Start[tt],ratio$Ratio[tt]*ploidy,pch = ".",col = colors()[136])
+
+        tt <- which(ratio$Chromosome==i  & ratio$Ratio==maxLevelToPlot & ratio$CopyNumber>ploidy)
+        points(ratio$Start[tt],ratio$Ratio[tt]*ploidy,pch = ".",col = colors()[136],cex=4)
+
+        tt <- which(ratio$Chromosome==i  & ratio$CopyNumber<ploidy & ratio$CopyNumber!= -1)
+        points(ratio$Start[tt],ratio$Ratio[tt]*ploidy,pch = ".",col = colors()[461])
+        tt <- which(ratio$Chromosome==i)
+
+        #UNCOMMENT HERE TO SEE THE PREDICTED COPY NUMBER LEVEL:
+        #points(ratio$Start[tt],ratio$CopyNumber[tt], pch = ".", col = colors()[24],cex=4)
+    }
+    #tt <- which(ratio$Chromosome==i)
+
+	#UNCOMMENT HERE TO SEE THE EVALUATED MEDIAN LEVEL PER SEGMENT:
+	#points(ratio$Start[tt],ratio$MedianRatio[tt]*ploidy, pch = ".", col = colors()[463],cex=4)
+
+    dev.off()
+}
+
+
+
+
+