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biopet.biopet
Commits
2865ff53
Commit
2865ff53
authored
Nov 17, 2014
by
Peter van 't Hof
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Added option to disable raw caller
parent
109ff2b3
Changes
2
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2 changed files
with
31 additions
and
24 deletions
+31
-24
biopet-framework/src/main/scala/nl/lumc/sasc/biopet/pipelines/gatk/GatkPipeline.scala
...ala/nl/lumc/sasc/biopet/pipelines/gatk/GatkPipeline.scala
+7
-4
biopet-framework/src/main/scala/nl/lumc/sasc/biopet/pipelines/gatk/GatkVariantcalling.scala
.../lumc/sasc/biopet/pipelines/gatk/GatkVariantcalling.scala
+24
-20
No files found.
biopet-framework/src/main/scala/nl/lumc/sasc/biopet/pipelines/gatk/GatkPipeline.scala
View file @
2865ff53
...
...
@@ -89,17 +89,20 @@ class GatkPipeline(val root: Configurable) extends QScript with MultiSampleQScri
)
yield
file
val
allRawVcfFiles
=
for
((
sampleID
,
sampleOutput
)
<-
samplesOutput
)
yield
sampleOutput
.
variantcalling
.
rawFilterVcfFile
val
cvRaw
=
CombineVariants
(
this
,
allRawVcfFiles
.
toList
,
outputDir
+
"variantcalling/multisample.raw.vcf.gz"
)
add
(
cvRaw
)
val
gatkVariantcalling
=
new
GatkVariantcalling
(
this
)
{
override
protected
lazy
val
configName
=
"gatkvariantcalling"
override
def
configPath
:
List
[
String
]
=
"multisample"
::
super
.
configPath
}
if
(
gatkVariantcalling
.
useMpileup
)
{
val
cvRaw
=
CombineVariants
(
this
,
allRawVcfFiles
.
toList
,
outputDir
+
"variantcalling/multisample.raw.vcf.gz"
)
add
(
cvRaw
)
gatkVariantcalling
.
rawVcfInput
=
cvRaw
.
out
}
gatkVariantcalling
.
preProcesBams
=
Some
(
false
)
gatkVariantcalling
.
doublePreProces
=
Some
(
false
)
gatkVariantcalling
.
inputBams
=
allBamfiles
.
toList
gatkVariantcalling
.
rawVcfInput
=
cvRaw
.
out
gatkVariantcalling
.
outputDir
=
outputDir
+
"variantcalling"
gatkVariantcalling
.
outputName
=
"multisample"
gatkVariantcalling
.
init
...
...
biopet-framework/src/main/scala/nl/lumc/sasc/biopet/pipelines/gatk/GatkVariantcalling.scala
View file @
2865ff53
...
...
@@ -41,6 +41,7 @@ class GatkVariantcalling(val root: Configurable) extends QScript with BiopetQScr
var
useHaplotypecaller
:
Option
[
Boolean
]
=
config
(
"use_haplotypecaller"
,
default
=
true
)
var
useUnifiedGenotyper
:
Option
[
Boolean
]
=
config
(
"use_unifiedgenotyper"
,
default
=
false
)
var
useAllelesOption
:
Option
[
Boolean
]
=
config
(
"use_alleles_option"
,
default
=
false
)
var
useMpileup
:
Option
[
Boolean
]
=
config
(
"use_mpileup"
,
default
=
true
)
def
init
()
{
if
(
outputName
==
null
&&
sampleID
!=
null
)
outputName
=
sampleID
...
...
@@ -113,27 +114,30 @@ class GatkVariantcalling(val root: Configurable) extends QScript with BiopetQScr
}
// Generate raw vcf
if
(
sampleID
!=
null
&&
scriptOutput
.
bamFiles
.
size
==
1
)
{
val
m2v
=
new
MpileupToVcf
(
this
)
m2v
.
inputBam
=
scriptOutput
.
bamFiles
.
head
m2v
.
sample
=
sampleID
m2v
.
output
=
outputDir
+
outputName
+
".raw.vcf"
add
(
m2v
)
scriptOutput
.
rawVcfFile
=
m2v
.
output
val
vcfFilter
=
new
VcfFilter
(
this
)
vcfFilter
.
defaults
++=
Map
(
"min_sample_depth"
->
8
,
"min_alternate_depth"
->
2
,
"min_samples_pass"
->
1
,
"filter_ref_calls"
->
true
)
vcfFilter
.
inputVcf
=
m2v
.
output
vcfFilter
.
outputVcf
=
this
.
swapExt
(
outputDir
,
m2v
.
output
,
".vcf"
,
".filter.vcf.gz"
)
add
(
vcfFilter
)
scriptOutput
.
rawFilterVcfFile
=
vcfFilter
.
outputVcf
}
else
if
(
rawVcfInput
!=
null
)
scriptOutput
.
rawFilterVcfFile
=
rawVcfInput
if
(
scriptOutput
.
rawFilterVcfFile
==
null
)
throw
new
IllegalStateException
(
"Files can't be empty"
)
mergBuffer
+=
(
"9.raw"
->
scriptOutput
.
rawFilterVcfFile
)
if
(
useMpileup
)
{
if
(
sampleID
!=
null
&&
scriptOutput
.
bamFiles
.
size
==
1
)
{
val
m2v
=
new
MpileupToVcf
(
this
)
m2v
.
inputBam
=
scriptOutput
.
bamFiles
.
head
m2v
.
sample
=
sampleID
m2v
.
output
=
outputDir
+
outputName
+
".raw.vcf"
add
(
m2v
)
scriptOutput
.
rawVcfFile
=
m2v
.
output
val
vcfFilter
=
new
VcfFilter
(
this
)
vcfFilter
.
defaults
++=
Map
(
"min_sample_depth"
->
8
,
"min_alternate_depth"
->
2
,
"min_samples_pass"
->
1
,
"filter_ref_calls"
->
true
)
vcfFilter
.
inputVcf
=
m2v
.
output
vcfFilter
.
outputVcf
=
this
.
swapExt
(
outputDir
,
m2v
.
output
,
".vcf"
,
".filter.vcf.gz"
)
add
(
vcfFilter
)
scriptOutput
.
rawFilterVcfFile
=
vcfFilter
.
outputVcf
}
else
if
(
rawVcfInput
!=
null
)
scriptOutput
.
rawFilterVcfFile
=
rawVcfInput
if
(
scriptOutput
.
rawFilterVcfFile
==
null
)
throw
new
IllegalStateException
(
"Files can't be empty"
)
mergBuffer
+=
(
"9.raw"
->
scriptOutput
.
rawFilterVcfFile
)
}
// Allele mode
if
(
useAllelesOption
.
get
)
{
val
mergeAlleles
=
MergeAlleles
(
this
,
mergeList
.
toList
,
outputDir
+
"raw.allele__temp_only.vcf.gz"
)
add
(
mergeAlleles
,
isIntermediate
=
true
)
...
...
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