Added flexiprep as separated artifact

public/flexiprep/.gitignore

+/target/
\ No newline at end of file

public/flexiprep/pom.xml

+<project xmlns="http://maven.apache.org/POM/4.0.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
+         xsi:schemaLocation="http://maven.apache.org/POM/4.0.0 http://maven.apache.org/xsd/maven-4.0.0.xsd">
+    <modelVersion>4.0.0</modelVersion>
+
+    <groupId>nl.lumc.sasc</groupId>
+    <artifactId>Flexiprep</artifactId>
+    <version>0.2.0-DEV</version>
+    <packaging>jar</packaging>
+    
+    <inceptionYear>2014</inceptionYear>
+    <name>Flexiprep</name>
+    <url>http://maven.apache.org</url>
+
+    <properties>
+        <project.build.sourceEncoding>UTF-8</project.build.sourceEncoding>
+        <sting.unpack.phase>prepare-package</sting.unpack.phase>
+        <sting.shade.phase>package</sting.shade.phase>
+        <app.main.class>nl.lumc.sasc.biopet.core.BiopetExecutable</app.main.class>
+    </properties>
+
+    <dependencies>
+        <dependency>
+            <groupId>nl.lumc.sasc</groupId>
+            <artifactId>BiopetFramework</artifactId>
+            <version>0.2.0-DEV</version>
+        </dependency>
+    </dependencies>
+    <build>
+        <resources>
+            <resource>
+                <directory>src/main/resources</directory>
+                <includes>
+                    <include>**/*</include>
+                </includes>
+            </resource>
+        </resources>
+        <plugins>
+            <plugin>
+                <groupId>org.scala-tools</groupId>
+                <artifactId>maven-scala-plugin</artifactId>
+                <version>2.15.2</version>
+                <executions>
+                    <execution>
+                        <id>scala-compile</id>
+                        <goals>
+                            <goal>compile</goal>
+                            <goal>testCompile</goal>
+                        </goals>
+                        <configuration>
+                            <args>
+                                <arg>-dependencyfile</arg>
+                                <arg>${project.build.directory}/.scala_dependencies</arg>
+                                <arg>-deprecation</arg>
+                                <arg>-feature</arg>
+                            </args>
+                        </configuration>
+                    </execution>
+                </executions>
+            </plugin>
+            <plugin>
+                <groupId>org.apache.maven.plugins</groupId>
+                <artifactId>maven-jar-plugin</artifactId>
+                <version>2.5</version>
+                <configuration>
+                    <archive>
+                        <manifest>
+                            <addDefaultImplementationEntries>true</addDefaultImplementationEntries>
+                            <addDefaultSpecificationEntries>true</addDefaultSpecificationEntries>
+                        </manifest>
+                    </archive>
+                </configuration>
+            </plugin>
+            <plugin>
+                <groupId>org.apache.maven.plugins</groupId>
+                <artifactId>maven-compiler-plugin</artifactId>
+                <version>2.3.2</version>
+                <configuration>
+                    <showDeprecation>true</showDeprecation>
+                </configuration>
+            </plugin>
+        </plugins>
+    </build>
+</project>

public/flexiprep/src/main/scala/nl/lumc/sasc/biopet/pipelines/flexiprep/Cutadapt.scala

+package nl.lumc.sasc.biopet.pipelines.flexiprep
+
+import scala.io.Source
+
+import nl.lumc.sasc.biopet.extensions.Ln
+import org.broadinstitute.gatk.utils.commandline.{ Input }
+
+import argonaut._, Argonaut._
+import scalaz._, Scalaz._
+
+import java.io.File
+import nl.lumc.sasc.biopet.core.config.Configurable
+import scala.collection.mutable.Map
+
+class Cutadapt(root: Configurable) extends nl.lumc.sasc.biopet.extensions.Cutadapt(root) {
+  @Input(doc = "Fastq contams file", required = false)
+  var contams_file: File = _
+
+  override def beforeCmd() {
+    super.beforeCmd
+    getContamsFromFile
+  }
+
+  override def cmdLine = {
+    if (!opt_adapter.isEmpty || !opt_anywhere.isEmpty || !opt_front.isEmpty) {
+      analysisName = getClass.getSimpleName
+      super.cmdLine
+    } else {
+      analysisName = getClass.getSimpleName + "-ln"
+      Ln(this, fastq_input, fastq_output, relative = true).cmd
+    }
+  }
+
+  def getContamsFromFile {
+    if (contams_file != null) {
+      if (contams_file.exists()) {
+        for (line <- Source.fromFile(contams_file).getLines) {
+          var s: String = line.substring(line.lastIndexOf("\t") + 1, line.size)
+          if (default_clip_mode == "3") opt_adapter += s
+          else if (default_clip_mode == "5") opt_front += s
+          else if (default_clip_mode == "both") opt_anywhere += s
+          else {
+            opt_adapter += s
+            logger.warn("Option default_clip_mode should be '3', '5' or 'both', falling back to default: '3'")
+          }
+          logger.info("Adapter: " + s + " found in: " + fastq_input)
+        }
+      } else logger.warn("File : " + contams_file + " does not exist")
+    }
+  }
+
+  def getSummary: Json = {
+    val trimR = """.*Trimmed reads: *(\d*) .*""".r
+    val tooShortR = """.*Too short reads: *(\d*) .*""".r
+    val tooLongR = """.*Too long reads: *(\d*) .*""".r
+    val adapterR = """Adapter '([C|T|A|G]*)'.*trimmed (\d*) times.""".r
+
+    var stats: Map[String, Int] = Map("trimmed" -> 0, "tooshort" -> 0, "toolong" -> 0)
+    var adapter_stats: Map[String, Int] = Map()
+
+    if (stats_output.exists) for (line <- Source.fromFile(stats_output).getLines) {
+      line match {
+        case trimR(m)                 => stats += ("trimmed" -> m.toInt)
+        case tooShortR(m)             => stats += ("tooshort" -> m.toInt)
+        case tooLongR(m)              => stats += ("toolong" -> m.toInt)
+        case adapterR(adapter, count) => adapter_stats += (adapter -> count.toInt)
+        case _                        =>
+      }
+    }
+    return ("num_reads_affected" := stats("trimmed")) ->:
+      ("num_reads_discarded_too_short" := stats("tooshort")) ->:
+      ("num_reads_discarded_too_long" := stats("toolong")) ->:
+      ("adapters" := adapter_stats.toMap) ->:
+      jEmptyObject
+  }
+}
+
+object Cutadapt {
+  def apply(root: Configurable, input: File, output: File): Cutadapt = {
+    val cutadapt = new Cutadapt(root)
+    cutadapt.fastq_input = input
+    cutadapt.fastq_output = output
+    cutadapt.stats_output = new File(output.getAbsolutePath.substring(0, output.getAbsolutePath.lastIndexOf(".")) + ".stats")
+    return cutadapt
+  }
+
+  def mergeSummaries(jsons: List[Json]): Json = {
+    var affected = 0
+    var tooShort = 0
+    var tooLong = 0
+    var adapter_stats: Map[String, Int] = Map()
+
+    for (json <- jsons) {
+      affected += json.field("num_reads_affected").get.numberOrZero.toInt
+      tooShort += json.field("num_reads_discarded_too_short").get.numberOrZero.toInt
+      tooLong += json.field("num_reads_discarded_too_long").get.numberOrZero.toInt
+
+      val adapters = json.fieldOrEmptyObject("adapters")
+      for (key <- adapters.objectFieldsOrEmpty) {
+        val number = adapters.field(key).get.numberOrZero.toInt
+        if (adapter_stats.contains(key)) adapter_stats(key) += number
+        else adapter_stats += (key -> number)
+      }
+    }
+
+    return ("num_reads_affected" := affected) ->:
+      ("num_reads_discarded_too_short" := tooShort) ->:
+      ("num_reads_discarded_too_long" := tooLong) ->:
+      ("adapters" := adapter_stats.toMap) ->:
+      jEmptyObject
+  }
+}

public/flexiprep/src/main/scala/nl/lumc/sasc/biopet/pipelines/flexiprep/Fastqc.scala

+/*
+ * To change this license header, choose License Headers in Project Properties.
+ * To change this template file, choose Tools | Templates
+ * and open the template in the editor.
+ */
+
+package nl.lumc.sasc.biopet.pipelines.flexiprep
+
+import java.io.File
+import nl.lumc.sasc.biopet.core.config.Configurable
+import scala.io.Source
+
+import argonaut._, Argonaut._
+import scalaz._, Scalaz._
+
+class Fastqc(root: Configurable) extends nl.lumc.sasc.biopet.extensions.Fastqc(root) {
+  def getDataBlock(name: String): Array[String] = { // Based on Fastqc v0.10.1
+    val outputDir = output.getAbsolutePath.stripSuffix(".zip")
+    val dataFile = new File(outputDir + "/fastqc_data.txt")
+    if (!dataFile.exists) return null
+    val data = Source.fromFile(dataFile).mkString
+    for (block <- data.split(">>END_MODULE\n")) {
+      val b = if (block.startsWith("##FastQC")) block.substring(block.indexOf("\n") + 1) else block
+      if (b.startsWith(">>" + name))
+        return for (line <- b.split("\n"))
+          yield line
+    }
+    return null
+  }
+
+  def getEncoding: String = {
+    val block = getDataBlock("Basic Statistics")
+    if (block == null) return null
+    for (
+      line <- block if (line.startsWith("Encoding"))
+    ) return line.stripPrefix("Encoding\t")
+    return null // Could be default Sanger with a warning in the log
+  }
+
+  def getSummary: Json = {
+    val subfixs = Map("plot_duplication_levels" -> "Images/duplication_levels.png",
+      "plot_kmer_profiles" -> "Images/kmer_profiles.png",
+      "plot_per_base_gc_content" -> "Images/per_base_gc_content.png",
+      "plot_per_base_n_content" -> "Images/per_base_n_content.png",
+      "plot_per_base_quality" -> "Images/per_base_quality.png",
+      "plot_per_base_sequence_content" -> "Images/per_base_sequence_content.png",
+      "plot_per_sequence_gc_content" -> "Images/per_sequence_gc_content.png",
+      "plot_per_sequence_quality" -> "Images/per_sequence_quality.png",
+      "plot_sequence_length_distribution" -> "Images/sequence_length_distribution.png",
+      "fastqc_data" -> "fastqc_data.txt")
+    val dir = output.getAbsolutePath.stripSuffix(".zip") + "/"
+    var outputMap: Map[String, Map[String, String]] = Map()
+    for ((k, v) <- subfixs) outputMap += (k -> Map("path" -> (dir + v)))
+
+    val temp = ("" := outputMap) ->: jEmptyObject
+    return temp.fieldOrEmptyObject("")
+  }
+}
+
+object Fastqc {
+  def apply(root: Configurable, fastqfile: File, outDir: String): Fastqc = {
+    val fastqcCommand = new Fastqc(root)
+    fastqcCommand.fastqfile = fastqfile
+    var filename: String = fastqfile.getName()
+    if (filename.endsWith(".gz")) filename = filename.substring(0, filename.size - 3)
+    if (filename.endsWith(".gzip")) filename = filename.substring(0, filename.size - 5)
+    if (filename.endsWith(".fastq")) filename = filename.substring(0, filename.size - 6)
+    //if (filename.endsWith(".fq")) filename = filename.substring(0,filename.size - 3)
+    fastqcCommand.output = new File(outDir + "/" + filename + "_fastqc.zip")
+    fastqcCommand.afterGraph
+    return fastqcCommand
+  }
+}

public/flexiprep/src/main/scala/nl/lumc/sasc/biopet/pipelines/flexiprep/Flexiprep.scala

+package nl.lumc.sasc.biopet.pipelines.flexiprep
+
+import org.broadinstitute.gatk.queue.QScript
+import org.broadinstitute.gatk.utils.commandline.{ Input, Argument }
+
+import nl.lumc.sasc.biopet.core.{ BiopetQScript, PipelineCommand }
+import nl.lumc.sasc.biopet.core.config.Configurable
+import nl.lumc.sasc.biopet.extensions.{ Gzip, Pbzip2, Md5sum, Zcat, Seqstat }
+import nl.lumc.sasc.biopet.scripts.{ FastqSync, FastqcToContams }
+
+class Flexiprep(val root: Configurable) extends QScript with BiopetQScript {
+  def this() = this(null)
+
+  @Input(doc = "R1 fastq file (gzipped allowed)", shortName = "R1", required = true)
+  var input_R1: File = _
+
+  @Input(doc = "R2 fastq file (gzipped allowed)", shortName = "R2", required = false)
+  var input_R2: File = _
+
+  @Argument(doc = "Skip Trim fastq files", shortName = "skiptrim", required = false)
+  var skipTrim: Boolean = config("skiptrim", default = false)
+
+  @Argument(doc = "Skip Clip fastq files", shortName = "skipclip", required = false)
+  var skipClip: Boolean = config("skipclip", default = false)
+
+  @Argument(doc = "Sample name", shortName = "sample", required = true)
+  var sampleName: String = _
+
+  @Argument(doc = "Library name", shortName = "library", required = true)
+  var libraryName: String = _
+
+  var paired: Boolean = (input_R2 != null)
+  var R1_ext: String = _
+  var R2_ext: String = _
+  var R1_name: String = _
+  var R2_name: String = _
+
+  var fastqc_R1: Fastqc = _
+  var fastqc_R2: Fastqc = _
+  var fastqc_R1_after: Fastqc = _
+  var fastqc_R2_after: Fastqc = _
+
+  val summary = new FlexiprepSummary(this)
+
+  def init() {
+    if (input_R1 == null) throw new IllegalStateException("Missing R1 on flexiprep module")
+    if (outputDir == null) throw new IllegalStateException("Missing Output directory on flexiprep module")
+    if (sampleName == null) throw new IllegalStateException("Missing Sample name on flexiprep module")
+    if (libraryName == null) throw new IllegalStateException("Missing Library name on flexiprep module")
+    else if (!outputDir.endsWith("/")) outputDir += "/"
+    paired = (input_R2 != null)
+
+    if (input_R1.endsWith(".gz")) R1_name = input_R1.getName.substring(0, input_R1.getName.lastIndexOf(".gz"))
+    else if (input_R1.endsWith(".gzip")) R1_name = input_R1.getName.substring(0, input_R1.getName.lastIndexOf(".gzip"))
+    else R1_name = input_R1.getName
+    R1_ext = R1_name.substring(R1_name.lastIndexOf("."), R1_name.size)
+    R1_name = R1_name.substring(0, R1_name.lastIndexOf(R1_ext))
+
+    if (paired) {
+      if (input_R2.endsWith(".gz")) R2_name = input_R2.getName.substring(0, input_R2.getName.lastIndexOf(".gz"))
+      else if (input_R2.endsWith(".gzip")) R2_name = input_R2.getName.substring(0, input_R2.getName.lastIndexOf(".gzip"))
+      else R2_name = input_R2.getName
+      R2_ext = R2_name.substring(R2_name.lastIndexOf("."), R2_name.size)
+      R2_name = R2_name.substring(0, R2_name.lastIndexOf(R2_ext))
+    }
+
+    summary.out = outputDir + sampleName + "-" + libraryName + ".qc.summary.json"
+  }
+
+  def biopetScript() {
+    runInitialJobs()
+
+    val out = if (paired) runTrimClip(outputFiles("fastq_input_R1"), outputFiles("fastq_input_R2"), outputDir)
+    else runTrimClip(outputFiles("fastq_input_R1"), outputDir)
+
+    val R1_files = for ((k, v) <- outputFiles if k.endsWith("output_R1")) yield v
+    val R2_files = for ((k, v) <- outputFiles if k.endsWith("output_R2")) yield v
+    runFinalize(R1_files.toList, R2_files.toList)
+  }
+
+  def runInitialJobs() {
+    outputFiles += ("fastq_input_R1" -> extractIfNeeded(input_R1, outputDir))
+    if (paired) outputFiles += ("fastq_input_R2" -> extractIfNeeded(input_R2, outputDir))
+
+    fastqc_R1 = Fastqc(this, input_R1, outputDir + "/" + R1_name + ".fastqc/")
+    add(fastqc_R1)
+    summary.addFastqc(fastqc_R1)
+    outputFiles += ("fastqc_R1" -> fastqc_R1.output)
+    outputFiles += ("contams_R1" -> getContams(fastqc_R1, R1_name))
+
+    val md5sum_R1 = Md5sum(this, input_R1, outputDir)
+    add(md5sum_R1)
+    summary.addMd5sum(md5sum_R1, R2 = false, after = false)
+
+    if (paired) {
+      fastqc_R2 = Fastqc(this, input_R2, outputDir + "/" + R2_name + ".fastqc/")
+      add(fastqc_R2)
+      summary.addFastqc(fastqc_R2, R2 = true)
+      outputFiles += ("fastqc_R2" -> fastqc_R2.output)
+      outputFiles += ("contams_R2" -> getContams(fastqc_R2, R2_name))
+
+      val md5sum_R2 = Md5sum(this, input_R2, outputDir)
+      add(md5sum_R2)
+      summary.addMd5sum(md5sum_R2, R2 = true, after = false)
+    }
+  }
+
+  def getContams(fastqc: Fastqc, pairname: String): File = {
+    val fastqcToContams = new FastqcToContams(this)
+    fastqcToContams.fastqc_output = fastqc.output
+    fastqcToContams.out = new File(outputDir + pairname + ".contams.txt")
+    fastqcToContams.contams_file = fastqc.contaminants
+    add(fastqcToContams)
+    return fastqcToContams.out
+  }
+
+  def runTrimClip(R1_in: File, outDir: String, chunk: String): (File, File, List[File]) = {
+    runTrimClip(R1_in, new File(""), outDir, chunk)
+  }
+  def runTrimClip(R1_in: File, outDir: String): (File, File, List[File]) = {
+    runTrimClip(R1_in, new File(""), outDir, "")
+  }
+  def runTrimClip(R1_in: File, R2_in: File, outDir: String): (File, File, List[File]) = {
+    runTrimClip(R1_in, R2_in, outDir, "")
+  }
+  def runTrimClip(R1_in: File, R2_in: File, outDir: String, chunkarg: String): (File, File, List[File]) = {
+    val chunk = if (chunkarg.isEmpty || chunkarg.endsWith("_")) chunkarg else chunkarg + "_"
+    var results: Map[String, File] = Map()
+
+    var R1: File = new File(R1_in)
+    var R2: File = new File(R2_in)
+    var deps: List[File] = if (paired) List(R1, R2) else List(R1)
+
+    val seqtkSeq_R1 = SeqtkSeq.apply(this, R1, swapExt(outDir, R1, R1_ext, ".sanger" + R1_ext), fastqc_R1)
+    add(seqtkSeq_R1, isIntermediate = true)
+    R1 = seqtkSeq_R1.output
+    deps ::= R1
+
+    if (paired) {
+      val seqtkSeq_R2 = SeqtkSeq.apply(this, R2, swapExt(outDir, R2, R2_ext, ".sanger" + R2_ext), fastqc_R2)
+      add(seqtkSeq_R2, isIntermediate = true)
+      R2 = seqtkSeq_R2.output
+      deps ::= R2
+    }
+
+    val seqstat_R1 = Seqstat(this, R1, outDir)
+    add(seqstat_R1, isIntermediate = true)
+    summary.addSeqstat(seqstat_R1, R2 = false, after = false, chunk)
+
+    if (paired) {
+      val seqstat_R2 = Seqstat(this, R2, outDir)
+      add(seqstat_R2, isIntermediate = true)
+      summary.addSeqstat(seqstat_R2, R2 = true, after = false, chunk)
+    }
+
+    if (!skipClip) { // Adapter clipping
+
+      val cutadapt_R1 = Cutadapt(this, R1, swapExt(outDir, R1, R1_ext, ".clip" + R1_ext))
+      if (outputFiles.contains("contams_R1")) cutadapt_R1.contams_file = outputFiles("contams_R1")
+      add(cutadapt_R1, isIntermediate = true)
+      summary.addCutadapt(cutadapt_R1, R2 = false, chunk)
+      R1 = cutadapt_R1.fastq_output
+      deps ::= R1
+      outputFiles += ("cutadapt_R1_stats" -> cutadapt_R1.stats_output)
+
+      if (paired) {
+        val cutadapt_R2 = Cutadapt(this, R2, swapExt(outDir, R2, R2_ext, ".clip" + R2_ext))
+        outputFiles += ("cutadapt_R2_stats" -> cutadapt_R2.stats_output)
+        if (outputFiles.contains("contams_R2")) cutadapt_R2.contams_file = outputFiles("contams_R2")
+        add(cutadapt_R2, isIntermediate = true)
+        summary.addCutadapt(cutadapt_R2, R2 = true, chunk)
+        R2 = cutadapt_R2.fastq_output
+        deps ::= R2
+
+        val fastqSync = FastqSync(this, cutadapt_R1.fastq_input, cutadapt_R1.fastq_output, cutadapt_R2.fastq_output,
+          swapExt(outDir, R1, R1_ext, ".sync" + R1_ext), swapExt(outDir, R2, R2_ext, ".sync" + R2_ext), swapExt(outDir, R1, R1_ext, ".sync.stats"))
+        fastqSync.deps :::= deps
+        add(fastqSync, isIntermediate = true)
+        summary.addFastqcSync(fastqSync, chunk)
+        outputFiles += ("syncStats" -> fastqSync.output_stats)
+        R1 = fastqSync.output_R1
+        R2 = fastqSync.output_R2
+        deps :::= R1 :: R2 :: Nil
+      }
+    }
+
+    if (!skipTrim) { // Quality trimming
+      val sickle = new Sickle(this)
+      sickle.input_R1 = R1
+      sickle.output_R1 = swapExt(outDir, R1, R1_ext, ".trim" + R1_ext)
+      if (paired) {
+        sickle.input_R2 = R2
+        sickle.output_R2 = swapExt(outDir, R2, R2_ext, ".trim" + R2_ext)
+        sickle.output_singles = swapExt(outDir, R2, R2_ext, ".trim.singles" + R1_ext)
+      }
+      sickle.output_stats = swapExt(outDir, R1, R1_ext, ".trim.stats")
+      sickle.deps = deps
+      add(sickle, isIntermediate = true)
+      summary.addSickle(sickle, chunk)
+      R1 = sickle.output_R1
+      if (paired) R2 = sickle.output_R2
+    }
+
+    val seqstat_R1_after = Seqstat(this, R1, outDir)
+    seqstat_R1_after.deps = deps
+    add(seqstat_R1_after)
+    summary.addSeqstat(seqstat_R1_after, R2 = false, after = true, chunk)
+
+    if (paired) {
+      val seqstat_R2_after = Seqstat(this, R2, outDir)
+      seqstat_R2_after.deps = deps
+      add(seqstat_R2_after)
+      summary.addSeqstat(seqstat_R2_after, R2 = true, after = true, chunk)
+    }
+
+    outputFiles += (chunk + "output_R1" -> R1)
+    if (paired) outputFiles += (chunk + "output_R2" -> R2)
+    return (R1, R2, deps)
+  }
+
+  def runFinalize(fastq_R1: List[File], fastq_R2: List[File]) {
+    if (fastq_R1.length != fastq_R2.length && paired) throw new IllegalStateException("R1 and R2 file number is not the same")
+    val R1 = new File(outputDir + R1_name + ".qc" + R1_ext + ".gz")
+    val R2 = new File(outputDir + R2_name + ".qc" + R2_ext + ".gz")
+
+    add(Gzip(this, fastq_R1, R1))
+    if (paired) add(Gzip(this, fastq_R2, R2))
+
+    outputFiles += ("output_R1_gzip" -> R1)
+    if (paired) outputFiles += ("output_R2_gzip" -> R2)
+
+    if (!skipTrim || !skipClip) {
+      val md5sum_R1 = Md5sum(this, R1, outputDir)
+      add(md5sum_R1)
+      summary.addMd5sum(md5sum_R1, R2 = false, after = true)
+      if (paired) {
+        val md5sum_R2 = Md5sum(this, R2, outputDir)
+        add(md5sum_R2)
+        summary.addMd5sum(md5sum_R2, R2 = true, after = true)
+      }
+      fastqc_R1_after = Fastqc(this, R1, outputDir + "/" + R1_name + ".qc.fastqc/")
+      add(fastqc_R1_after)
+      summary.addFastqc(fastqc_R1_after, after = true)
+      if (paired) {
+        fastqc_R2_after = Fastqc(this, R2, outputDir + "/" + R2_name + ".qc.fastqc/")
+        add(fastqc_R2_after)
+        summary.addFastqc(fastqc_R2_after, R2 = true, after = true)
+      }
+    }
+
+    add(summary)
+  }
+
+  def extractIfNeeded(file: File, runDir: String): File = {
+    if (file.getName().endsWith(".gz") || file.getName().endsWith(".gzip")) {
+      var newFile: File = swapExt(runDir, file, ".gz", "")
+      if (file.getName().endsWith(".gzip")) newFile = swapExt(runDir, file, ".gzip", "")
+      val zcatCommand = Zcat(this, file, newFile)
+      zcatCommand.isIntermediate = true
+      add(zcatCommand)
+      return newFile
+    } else if (file.getName().endsWith(".bz2")) {
+      var newFile = swapExt(runDir, file, ".bz2", "")
+      val pbzip2 = Pbzip2(this, file, newFile)
+      pbzip2.isIntermediate = true
+      add(pbzip2)
+      return newFile
+    } else return file
+  }
+}
+
+object Flexiprep extends PipelineCommand

public/flexiprep/src/main/scala/nl/lumc/sasc/biopet/pipelines/flexiprep/FlexiprepSummary.scala

+package nl.lumc.sasc.biopet.pipelines.flexiprep
+
+import java.io.PrintWriter
+import nl.lumc.sasc.biopet.core.config.Configurable
+import nl.lumc.sasc.biopet.extensions.{ Md5sum, Seqstat }
+import nl.lumc.sasc.biopet.scripts.{ FastqSync }
+import org.broadinstitute.gatk.queue.function.InProcessFunction
+import org.broadinstitute.gatk.utils.commandline.{ Input, Output }
+import java.io.File
+import argonaut._, Argonaut._
+impor