Picard.scala 3.3 KB
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/**
 * Biopet is built on top of GATK Queue for building bioinformatic
 * pipelines. It is mainly intended to support LUMC SHARK cluster which is running
 * SGE. But other types of HPC that are supported by GATK Queue (such as PBS)
 * should also be able to execute Biopet tools and pipelines.
 *
 * Copyright 2014 Sequencing Analysis Support Core - Leiden University Medical Center
 *
 * Contact us at: sasc@lumc.nl
 *
 * A dual licensing mode is applied. The source code within this project that are
 * not part of GATK Queue is freely available for non-commercial use under an AGPL
 * license; For commercial users or users who do not want to follow the AGPL
 * license, please contact us to obtain a separate license.
 */
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package nl.lumc.sasc.biopet.extensions.picard
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import java.io.File

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import nl.lumc.sasc.biopet.core.BiopetJavaCommandLineFunction
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import org.broadinstitute.gatk.utils.commandline.{ Argument }
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import scala.io.Source

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/**
 * General picard extension
 *
 * This is based on using class files directly from the jar
 */
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abstract class Picard extends BiopetJavaCommandLineFunction {
  override def subPath = "picard" :: super.subPath

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  if (config.contains("picard_jar")) jarFile = config("picard_jar")

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  @Argument(doc = "VERBOSITY", required = false)
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  var verbosity: Option[String] = config("verbosity")
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  @Argument(doc = "QUIET", required = false)
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  var quiet: Boolean = config("quiet", default = false)
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  @Argument(doc = "VALIDATION_STRINGENCY", required = false)
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  var stringency: Option[String] = config("validationstringency")
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  @Argument(doc = "COMPRESSION_LEVEL", required = false)
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  var compression: Option[Int] = config("compressionlevel")
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  @Argument(doc = "MAX_RECORDS_IN_RAM", required = false)
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  var maxRecordsInRam: Option[Int] = config("maxrecordsinram")
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  @Argument(doc = "CREATE_INDEX", required = false)
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  var createIndex: Boolean = config("createindex", default = true)
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  @Argument(doc = "CREATE_MD5_FILE", required = false)
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  var createMd5: Boolean = config("createmd5", default = false)
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  override def versionCommand = {
    if (jarFile != null) executable + " -cp " + jarFile + " " + javaMainClass + " -h"
    else null
  }
  override val versionRegex = """Version: (.*)""".r
  override val versionExitcode = List(0, 1)
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  override val defaultVmem = "8G"
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  memoryLimit = Option(3.0)
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  override def commandLine = super.commandLine +
    required("TMP_DIR=" + jobTempDir) +
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    optional("VERBOSITY=", verbosity, spaceSeparated = false) +
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    conditional(quiet, "QUIET=TRUE") +
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    optional("VALIDATION_STRINGENCY=", stringency, spaceSeparated = false) +
    optional("COMPRESSION_LEVEL=", compression, spaceSeparated = false) +
    optional("MAX_RECORDS_IN_RAM=", maxRecordsInRam, spaceSeparated = false) +
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    conditional(createIndex, "CREATE_INDEX=TRUE") +
    conditional(createMd5, "CREATE_MD5_FILE=TRUE")
}
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object Picard {
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  /**
   * This function parse a metrics file in separated values
   * @param file input metrics file
   * @return (header, content)
   */
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  def getMetrics(file: File) = {
    val lines = Source.fromFile(file).getLines().toArray

    val start = lines.indexWhere(_.startsWith("## METRICS CLASS")) + 1
    val end = lines.indexOf("", start)

    val header = lines(start).split("\t")
    val content = (for (i <- (start + 1) until end) yield lines(i).split("\t")).toList

    (header, content)
  }
}