static/getprimers.sh: Tweak parameters for primer3 call based on VCGS recommendations

prinia/static/getprimers.sh

@@ -32,8 +32,8 @@ PRIMER_INTERNAL_WT_GC_PERCENT_GT=0.0
 PRIMER_GC_CLAMP=0
 PRIMER_MAX_END_GC=5
 PRIMER_OPT_SIZE=25
-PRIMER_MIN_SIZE=20
-PRIMER_MAX_SIZE=30
+PRIMER_MIN_SIZE=18
+PRIMER_MAX_SIZE=25
 PRIMER_MAX_NS_ACCEPTED=0
 PRIMER_PRODUCT_SIZE_RANGE=$1
 PRIMER_PRODUCT_OPT_SIZE=$opt_size
@@ -42,10 +42,11 @@ PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.1
 P3_FILE_FLAG=1
 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21
 PRIMER_EXPLAIN_FLAG=1
-PRIMER_MIN_TM=58
+PRIMER_MIN_TM=57
 PRIMER_MAX_TM=62
+PRIMER_OPT_TM=60
+PRIMER_PAIR_MAX_DIFF_TM=2
 PRIMER_NUM_RETURN=200
+PRIMER_MAX_HAIRPIN_TH=47
 =
 EOF
-
-