primer3_conf_default.txt 838 Bytes
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SEQUENCE_ID=prinia_template
SEQUENCE_TEMPLATE=ACTG
SEQUENCE_TARGET=50-60
SEQUENCE_EXCLUDED_REGION=50-60
PRIMER_TASK=pick_detection_primers
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_MIN_GC=20
PRIMER_INTERNAL_MIN_GC=20
PRIMER_OPT_GC_PERCENT=50
PRIMER_MAX_GC=80
PRIMER_INTERNAL_MAX_GC=80
PRIMER_WT_GC_PERCENT_LT=0
PRIMER_INTERNAL_WT_GC_PERCENT_LT=0
PRIMER_WT_GC_PERCENT_GT=0
PRIMER_INTERNAL_WT_GC_PERCENT_GT=0
PRIMER_GC_CLAMP=0
PRIMER_MAX_END_GC=5
PRIMER_OPT_SIZE=25
PRIMER_MIN_SIZE=20
PRIMER_MAX_SIZE=30
PRIMER_MAX_NS_ACCEPTED=0
PRIMER_PRODUCT_SIZE_RANGE=200-450
PRIMER_PRODUCT_OPT_SIZE=325
PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.1
PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.1
P3_FILE_FLAG=1
SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21
PRIMER_EXPLAIN_FLAG=1
PRIMER_MIN_TM=58
PRIMER_MAX_TM=62
PRIMER_NUM_RETURN=200