1. 30 Jul, 2015 1 commit
  2. 29 Jul, 2015 1 commit
    • jhoogenboom's avatar
      Introducing bgestimate · be745e64
      jhoogenboom authored
      I could write about all its features here, but instead I will point
      out some future plans to highlight the things that are possibly not
      optimal in their current implementation.
      
      There are a number of things I plan to change in the future:
      * The output format is currently JSON, perhaps a carefully designed
        tabular format is a better choice. The benefit of switching to a
        tabluar format is that the data can be loaded into e.g. Excel as
        well.
      * The profiles are currently produced separately for forward and
        reverse reads. I would prefer to integrate these into a single
        computation that estimates allele balance in the heterozygotes
        using both strands as well.
      * I would like to add information about strand bias of the alleles
        as well. The most straightforward way to do this is to set only
        the forward reads of the true allele to 100 and treat the reverse
        reads the same as all background products. You will then obtain a
        number of reverse reads observed for ever 100 forward reads of
        the true allele.
      * I think it would be appropriate to make sure the values in the
        allele balance matrices of each sample ('Ax' in the source code)
        should add up to 1. For homozygotes, it is currently a scalar 1,
        the sum of the elements tend to be more than 1. This means that a
        heterozygous sample has a stronger influence on the profiles than
        a homozygous sample.
      be745e64
  3. 27 Jul, 2015 1 commit
    • jhoogenboom's avatar
      Updates to allelefinder · f9543ed9
      jhoogenboom authored
      * Allelefinder can now combine data from multiple files into a
        single sample (this happens when the same sample tag was
        extracted from their names).
      * Allelefinder can now automatically convert sequences to a given
        format (this is optional though). This is particularly useful
        when combining the knownalleles.csv and newalleles.csv files of
        a sample. (Note that allelefinder still assumes that the files
        contain different alleles; no attempt is made to check whether
        the same allele was represented in multiple files.)
      f9543ed9
  4. 24 Jul, 2015 1 commit
  5. 23 Jul, 2015 1 commit
    • jhoogenboom's avatar
      Laying foundations · 160594c5
      jhoogenboom authored
      * Introducing a new, extended library file format to support
        allele name generation.  The new libconvert tool can convert
        TSSV libraries to the new format and vice versa.
      * Added functions for converting between raw sequences, TSSV-style
        sequences, and allele names.
      * Added global -d/--debug option.
      
      Stuttermark updates:
      * Stuttermark now automatically converts input sequences to
        TSSV-style if a library is provided.
      * Stuttermark will no longer crash if there is no 'name' column.
        Instead, all sequences are taken to belong to the same marker.
      
      New tools:
      * libconvert converts between FDSTools and TSSV library formats.
      * seqconvert converts between raw sequences, TSSV-style sequences,
        and allele names.
      * allelefinder detects the true alleles in reference samples.
      160594c5
  6. 02 Jul, 2015 1 commit
    • jhoogenboom's avatar
      Initial commit · 668970ed
      jhoogenboom authored
      FDSTools v0.0.1 with Stuttermark v1.3.
      Other tools will come later.
      668970ed