lib.py 63.7 KB
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#!/usr/bin/env python

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import re, sys, argparse, random, itertools
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#import numpy as np  # Imported only when calling nnls()
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from ConfigParser import RawConfigParser, MissingSectionHeaderError
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from StringIO import StringIO
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# Patterns that match entire sequences.
PAT_SEQ_RAW = re.compile("^[ACGT]*$")
PAT_SEQ_TSSV = re.compile("^(?:[ACGT]+\(\d+\))*$")
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PAT_SEQ_ALLELENAME_STR = re.compile(  # First line: n_ACT[m] or alias.
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    "^(?:(?:(?:CE)?-?\d+(?:\.\d+)?_(?:[ACGT]+\[\d+\])*)|((?!_).+?))"
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    "(?:_[-+]\d+(?:\.1)?(?P<a>(?:(?<=\.1)-)|(?<!\.1)[ACGT]+)>"  # _+3A>
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        "(?!(?P=a))(?:[ACGT]+|-))*$")  # Portion of variants after '>'.
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PAT_SEQ_ALLELENAME_SNP = re.compile(
    "^REF$|^(?:(?:(?<=^)|(?<!^) )"  # 'REF' or space-separated variants.
    "\d+(?:\.1)?(?P<a>(?:(?<=\.1)-)|(?<!\.1)[ACGT]+)>"
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        "(?!(?P=a))(?:[ACGT]+|-))+$")  # Portion of variants after '>'.
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PAT_SEQ_ALLELENAME_MT = re.compile(
    "^REF$|^(?:(?:(?<=^)|(?<!^) )"  # 'REF' or space-separated variants.
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    "(?:-?\d+\.\d+[ACGT]|(?P<a>[ACGT])?\d+(?(a)(?!(?P=a)))(?:[ACGT-]|del)))+$")
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# Patterns that match blocks of TSSV-style sequences and allele names.
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PAT_TSSV_BLOCK = re.compile("([ACGT]+)\((\d+)\)")
PAT_ALLELENAME_BLOCK = re.compile("([ACGT]+)\[(\d+)\]")
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PAT_ALIAS = re.compile("^(?!_).+$")
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# Patterns that match a single variant.
PAT_VARIANT_STR = re.compile(
    "^(?P<pos>[-+]\d+)(?:\.(?P<ins>1))?"
    "(?P<old>(?:(?<=\.1)-)|(?<!\.1)[ACGT]+)>"
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    "(?!(?P=old))(?P<new>[ACGT]+|-)$")
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PAT_VARIANT_SNP = re.compile(
    "^(?P<pos>\d+)(?:\.(?P<ins>1))?"
    "(?P<old>(?:(?<=\.1)-)|(?<!\.1)[ACGT]+)>"
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    "(?!(?P=old))(?P<new>[ACGT]+|-)$")
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PAT_VARIANT_MT = re.compile(
    "^(?P<old>(?P<a>[ACGT])|-?)"
    "(?P<pos>\d+)(?(a)|(?:\.(?P<ins>\d+))?)"
    "(?P<new>[ACGT-]|del)$")
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# Patterns that match (parts of) an STR definition.
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PAT_STR_DEF = re.compile("^(?:(?:(?<=^)|(?<!^)\s+)[ACGT]+\s+\d+\s+\d+)*$")
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PAT_STR_DEF_BLOCK = re.compile("([ACGT]+)\s+(\d+)\s+(\d+)")

# Pattern to split a comma-, semicolon-, or space-separated list.
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PAT_SPLIT = re.compile("\s*[,; \t]\s*")

# Pattern that matches a chromosome name/number.
PAT_CHROMOSOME = re.compile(
    "^(?:[Cc][Hh][Rr](?:[Oo][Mm])?)?([1-9XYM]|1\d|2[0-2])$")
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# Default regular expression to capture sample tags in file names.
# This is the default of the -e command line option.
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DEF_TAG_EXPR = "^(.*?)(?:\.[^.]+)?$"
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# Default formatting template to write sample tags.
# This is the default of the -f command line option.
DEF_TAG_FORMAT = "\\1"

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# Default formatting template to construct output file names for batch
# processing.  \1 and \2 refer to sample tag and tool name.
# This is the default for the -o command line option with batch support.
DEF_OUTFILE_FORMAT = "\\1-\\2.out"

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# IUPAC Table of complementary bases.
COMPL = {"A": "T", "T": "A", "U": "A", "G": "C", "C": "G", "R": "Y", "Y": "R",
         "K": "M", "M": "K", "B": "V", "V": "B", "D": "H", "H": "D",
         "a": "t", "t": "a", "u": "a", "g": "c", "c": "g", "r": "y", "y": "r",
         "k": "m", "m": "k", "b": "v", "v": "b", "d": "h", "h": "d"}

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# Special values that may appear in the place of a sequence.
SEQ_SPECIAL_VALUES = ("No data", "Other sequences")

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def get_genome_pos(location, x, invert=False):
    """Get the genome position of the x-th base in a sequence."""
    if invert:
        offset = 0
        for i in range(1, len(location)):
            if i % 2:
                # Starting position.
                pos = location[i]
            elif pos <= x <= location[i]:
                # x is in the current range
                break
            else:
                offset += location[i]-pos+1
        else:
            if len(location) % 2:
                raise ValueError("Position %i is outside sequence range" % x)
        return offset + x - pos
    else:
        for i in range(1, len(location)):
            if i % 2:
                # Starting position.
                pos = location[i]
            elif location[i]-pos < x:
                # x is after this ending position
                x -= location[i]-pos+1
            else:
                # x is before this ending position
                break
        return pos + x
#get_genome_pos


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def call_variants(template, sequence, location="suffix", cache=True,
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                  debug=False):
    """
    Perform a global alignment of sequence to template and return a
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    list of variants detected.  The format (nomenclature) of the
    returned variants depends on the location argument.

    If location is "suffix" (the default), all variants are given as
    substitutions in the form posX>Y, where the first base in the
    template is pos=1.  With location set to "prefix", bases are counted
    from right to left instead.  Insertions and deletions are written as
    pos.1->Y and posX>-, respectively.

    If location is a tuple ("M", position) with any integer for the
    position, variants are written following the mtDNA nomenclature
    guidelines.  The given position is that of the first base in the
    template.

    If location is a tuple ("chromosome name", position), a
    NotImplementedError is raised.
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    By default, the results of this function are cached.  Set cache to
    False to suppress caching the result and reduce memory usage.

    Setting debug to True will cause the alignment matrices to be
    printed to sys.stdout.  Be aware that they can be quite large.
    """
    # Saving the results in a cache to avoid repeating alignments.
    try:
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        return call_variants.cache[template, sequence, location]
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    except KeyError:
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        cache_key = location
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    row_offset = len(template) + 1
    matrix_match = [0] * row_offset * (len(sequence)+1)
    matrix_gap1 = [-sys.maxint-1] * row_offset * (len(sequence)+1)
    matrix_gap2 = [-sys.maxint-1] * row_offset * (len(sequence)+1)

    MATCH_SCORE = 1
    MISMATCH_SCORE = -1
    GAP_OPEN_SCORE = -10
    GAP_EXTEND_SCORE = -1
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    variant_format = "%i%s>%s"

    if location == "prefix":
        location = ("prefix", -len(template))
    elif location == "suffix":
        # Include plus signs for position numbers.
        variant_format = "%+i%s>%s"
        location = ("suffix", 1)
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    elif type(location) != tuple or len(location) < 2:
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        raise ValueError("Unknown location %r. It should be 'prefix', "
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            "'suffix', or a tuple (chromosome, position [, endpos])" %
            location)
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    for i in range(len(matrix_match)):
        x = i % row_offset
        y = i / row_offset

        # Initialisation of first row and column.
        if x == 0 or y == 0:
            if x != 0:
                # Top row.
                matrix_gap1[i] = GAP_OPEN_SCORE + GAP_EXTEND_SCORE * (x - 1)
                matrix_match[i] = matrix_gap1[i]
            elif y != 0:
                # Left column.
                matrix_gap2[i] = GAP_OPEN_SCORE + GAP_EXTEND_SCORE * (y - 1)
                matrix_match[i] = matrix_gap2[i]
            continue

        matrix_gap1[i] = max(
            matrix_match[i-1] + GAP_OPEN_SCORE,
            matrix_gap1[i-1] + GAP_EXTEND_SCORE)
        matrix_gap2[i] = max(
            matrix_match[i-row_offset] + GAP_OPEN_SCORE,
            matrix_gap2[i-row_offset] + GAP_EXTEND_SCORE)

        if template[x-1] == sequence[y-1]:
            match = MATCH_SCORE
        else:
            match = MISMATCH_SCORE

        matrix_match[i] = max(
            matrix_match[i-1-row_offset] + match,
            matrix_gap1[i],
            matrix_gap2[i])

    if debug:
        print("GAP1")
        for i in range(0, len(matrix_gap1), row_offset):
            print(("%5i" * row_offset) % tuple(matrix_gap1[i:i+row_offset]))
        print("GAP2")
        for i in range(0, len(matrix_gap2), row_offset):
            print(("%5i" * row_offset) % tuple(matrix_gap2[i:i+row_offset]))
        print("Match")
        for i in range(0, len(matrix_match), row_offset):
            print(("%5i" * row_offset) % tuple(matrix_match[i:i+row_offset]))


    # Backtracking.
    variants = []
    variant_template = 0
    variant_sequence = 0
    i = len(matrix_match) - 1
    while i >= 0:
        x = i % row_offset
        y = i / row_offset

        if matrix_gap1[i] == matrix_match[i]:
            # Go horizontally.  Deletion.
            variant_template += 1
            i -= 1
            continue

        if matrix_gap2[i] == matrix_match[i]:
            # Go vertically.  Insertion.
            variant_sequence += 1
            i -= row_offset
            continue

        # Only backtracking diagonally if a gap is out of the question.
        # Go diagonally.  Either match or mismatch.
        if i == 0 or template[x - 1] == sequence[y - 1]:
            # Match.  Flush variants.
            if variant_template or variant_sequence:
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                if location[0] == "M":
                    # MtDNA variants are one-base-at-a-time.
                    for j in range(
                            max(variant_template, variant_sequence)-1, -1, -1):
                        variants.append("%s%i%s%s" % (
                            template[x+j] if j < variant_template else "",#"-",
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                            get_genome_pos(
                                location, x + min(j, variant_template-1)),
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                            ".%i" % (j-variant_template+1)
                                if j >= variant_template else "",
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                            sequence[y+j] if j < variant_sequence else "del"))
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                elif variant_template == 0:
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                    # Insertions: "-131.1->C" instead of "-130->C".
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                    variants.append(variant_format % (
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                        get_genome_pos(location, x - 1),
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                        ".1-",
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                        sequence[y:y+variant_sequence]))
                else:
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                    variants.append(variant_format % (
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                        get_genome_pos(location, x),
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                        template[x:x+variant_template],
                        sequence[y:y+variant_sequence] or "-"))
                variant_template = 0
                variant_sequence = 0
        else:
            # Start/extend mismatch.
            variant_template += 1
            variant_sequence += 1
        i -= 1 + row_offset

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    # Variants were called from right to left.  Reverse their order.
    if location[0] != "prefix":
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        variants.reverse()

    # Store the result in the cache.
    if cache:
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        call_variants.cache[template, sequence, cache_key] = variants
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    return variants
#call_variants
call_variants.cache = {}


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def mutate_sequence(seq, variants, location=None):
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    """Apply the given variants to the given sequence."""
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    if type(location) != tuple or len(location) < 2:
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        pattern = PAT_VARIANT_STR
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        location = (None, 0)
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    elif location[0] == "M":
        pattern = PAT_VARIANT_MT
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        location = (location[0], location[1]-1) + tuple(location[2:])
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    else:
        pattern = PAT_VARIANT_SNP
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        location = (location[0], location[1]-1) + tuple(location[2:])
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    seq = [[]] + [[base] for base in seq]
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    for variant in variants:
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        vm = pattern.match(variant)
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        if vm is None:
            raise ValueError("Unrecognised variant '%s'" % variant)
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        pos = int(vm.group("pos"))
        ins = int(vm.group("ins") or 0)
        old = vm.group("old")
        new = vm.group("new")
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        if old == "-":
            old = ""
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        if new == "-" or new == "del":
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            new = ""
        if pos < 0:
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            pos += len(seq)
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        pos = get_genome_pos(location, pos, True)
        if pos < 0 or (pos == 0 and not ins) or pos >= len(seq):
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            raise ValueError(
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                "Position of variant '%s' is outside sequence range" %
                    (variant))
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        if (not ins and old and old != "".join("".join(x[:1])
                for x in seq[pos:pos+len(old)])):
            raise ValueError(
                "Incorrect original sequence in variant '%s'; should be '%s'!"
                % (variant, "".join("".join(x[:1])
                    for x in seq[pos:pos+len(old)])))
        elif not ins and not old:
            # MtDNA substitution with reference base omitted.
            old = "".join("".join(x[:1]) for x in seq[pos:pos+len(new)])
        if not ins:
            # Remove old bases, retaining those inserted between/after.
            seq[pos:pos+len(old)] = [
                [""] + x[1:] for x in seq[pos:pos+len(old)]]
            # Place new entirely in the position of the first old base.
            seq[pos][0] = new
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        else:
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            # Insert new exactly ins positions after pos.
            while len(seq[pos]) <= ins:
                seq[pos].append("")
            seq[pos][ins] = new
    return "".join("".join(x) for x in seq)
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#mutate_sequence


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def parse_library(libfile, stream=False):
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    try:
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        if not stream:
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            libfile = sys.stdin if libfile == "-" else open(libfile, "r")
        if libfile == sys.stdin:
            # Can't seek on pipes, so read it into a buffer first.
            libfile = StringIO(sys.stdin.read())
        try:
            library = parse_library_ini(libfile)
            if not stream:
                libfile.close()
            return library
        except MissingSectionHeaderError:
            # Not an ini file.
            pass
        libfile.seek(0)
        library = parse_library_tsv(libfile)
        if not stream and libfile != sys.stdin:
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            libfile.close()
        return library
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    except ValueError as err:
        raise argparse.ArgumentTypeError(err)
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#parse_library


def parse_library_tsv(handle):
    """
    Parse a TSSV library file (tab-separated values format).

    The provided file should contain at least four columns: marker name,
    left flanking sequence, right flanking sequence, and STR definition.

    Return a nested dict with top-level keys "flanks" and "regex".
    """
    library = {
      "flanks": {},
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      "regex": {},
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      "blocks_middle": {}
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    }
    for line in handle:
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        line = [x.strip() for x in line.rstrip("\r\n").split("\t")]
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        if line == [""]:
            continue
        if len(line) < 4:
            raise ValueError(
                "Invalid library file: encountered line with %i columns, "
                "need at least 4" % len(line))
        marker = line[0]
        if PAT_SEQ_RAW.match(line[1]) is None:
            raise ValueError("Flanking sequence '%s' of marker %s is invalid" %
                             (line[1], marker))
        if PAT_SEQ_RAW.match(line[2]) is None:
            raise ValueError("Flanking sequence '%s' of marker %s is invalid" %
                             (line[2], marker))
        if PAT_STR_DEF.match(line[3]) is None:
            raise ValueError("STR definition '%s' of marker %s is invalid" %
                             (line[3], marker))
        library["flanks"][marker] = line[1:3]
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        library["blocks_middle"][marker] = [
            (block[0], int(block[1]), int(block[2])) for block in
                PAT_STR_DEF_BLOCK.findall(line[3])]
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        library["regex"][marker] = re.compile(
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            "".join(("^", "".join(
                "(%s){%s,%s}" % x for x in PAT_STR_DEF_BLOCK.findall(line[3])),
                "$")))
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    return library
#parse_library_tsv


def parse_library_ini(handle):
    library = {
      "flanks": {},
      "prefix": {},
      "suffix": {},
      "regex": {},
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      "blocks_middle": {},
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      "nostr_reference": {},
      "genome_position": {},
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      "length_adjust": {},
      "block_length": {},
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      "max_expected_copies": {},
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      "expected_length": {},
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      "aliases": {}
    }
    markers = set()

    ini = RawConfigParser()
    ini.optionxform = str
    ini.readfp(handle)
    for section in ini.sections():
        for marker in ini.options(section):
            value = ini.get(section, marker)
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            section_low = section.lower()
            if section_low == "flanks":
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                values = PAT_SPLIT.split(value)
                if len(values) != 2:
                    raise ValueError(
                        "For marker %s, %i flanking sequences were given,"
                        "need exactly 2" % (marker, len(values)))
                for value in values:
                    if PAT_SEQ_RAW.match(value) is None:
                        raise ValueError(
                            "Flanking sequence '%s' of marker %s is invalid" %
                            (value, marker))
                library["flanks"][marker] = values
                markers.add(marker)
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            elif section_low == "prefix":
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                if marker in library["nostr_reference"]:
                    raise ValueError(
                        "A prefix was defined for non-STR marker %s" % marker)
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                values = PAT_SPLIT.split(value)
                for value in values:
                    if PAT_SEQ_RAW.match(value) is None:
                        raise ValueError(
                            "Prefix sequence '%s' of marker %s is invalid" %
                            (value, marker))
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                library["prefix"][marker] = values
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                markers.add(marker)
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            elif section_low == "suffix":
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                if marker in library["nostr_reference"]:
                    raise ValueError(
                        "A suffix was defined for non-STR marker %s" % marker)
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                values = PAT_SPLIT.split(value)
                for value in values:
                    if PAT_SEQ_RAW.match(value) is None:
                        raise ValueError(
                            "Suffix sequence '%s' of marker %s is invalid" %
                            (value, marker))
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                library["suffix"][marker] = values
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                markers.add(marker)
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            elif section_low == "genome_position":
                values = PAT_SPLIT.split(value)
                chromosome = PAT_CHROMOSOME.match(values[0])
                if chromosome is None:
                    raise ValueError(
                        "Invalid chromosome '%s' for marker %s." %
                        (values[0], marker))
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                pos = [chromosome.group(1)]
                for i in range(1, len(values)):
                    try:
                        pos.append(int(values[i]))
                    except:
                        raise ValueError(
                            "Position '%s' of marker %s is not a valid integer"
                            % (values[i], marker))
                    if not i % 2 and pos[-2] >= pos[-1]:
                        raise ValueError(
                            "End position %i of marker %s must be higher than "
                            "corresponding start position %i" %
                            (pos[-1], marker, pos[-2]))
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                if len(values) == 1:
                    pos.append(1)
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                library["genome_position"][marker] = tuple(pos)
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                markers.add(marker)
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            elif section_low == "length_adjust":
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                try:
                    value = int(value)
                except:
                    raise ValueError(
                        "Length adjustment '%s' of marker %s is not a valid "
                        "integer" % (value, marker))
                library["length_adjust"][marker] = value
                markers.add(marker)
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            elif section_low == "block_length":
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                try:
                    value = int(value)
                except:
                    raise ValueError(
                        "Block length '%s' of marker %s is not a valid integer"
                        % (value, marker))
                library["block_length"][marker] = value
                markers.add(marker)
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            elif section_low == "max_expected_copies":
                try:
                    value = int(value)
                except:
                    raise ValueError(
                        "Maximum number of expected copies '%s' of marker %s "
                        "is not a valid integer" % (value, marker))
                library["max_expected_copies"][marker] = value
                markers.add(marker)
            elif section_low == "aliases":
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                values = PAT_SPLIT.split(value)
                if len(values) != 3:
                    raise ValueError("Alias %s does not have 3 values, but %i"
                                     % (marker, len(values)))
                if PAT_SEQ_RAW.match(values[1]) is None:
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                    raise ValueError(
                        "Alias sequence '%s' of alias %s is invalid" %
                        (values[1], marker))
                if PAT_ALIAS.match(values[2]) is None:
                    raise ValueError(
                        "Allele name '%s' of alias %s is invalid" %
                        (values[2], marker))
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                library["aliases"][marker] = {
                    "marker": values[0],
                    "sequence": values[1],
                    "name": values[2]
                }
                markers.add(marker)
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            elif section_low == "repeat":
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                if marker in library["nostr_reference"]:
                    raise ValueError(
                        "Marker %s was encountered in both [repeat] and "
                        "[no_repeat] sections" % marker)
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                if PAT_STR_DEF.match(value) is None:
                    raise ValueError(
                        "STR definition '%s' of marker %s is invalid" %
                        (value, marker))
                library["regex"][marker] = value
                markers.add(marker)
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            elif section_low == "no_repeat":
                if marker in library["regex"]:
                    raise ValueError(
                        "Marker %s was encountered in both [repeat] and "
                        "[no_repeat] sections" % marker)
                if marker in library["prefix"] or marker in library["suffix"]:
                    raise ValueError(
                        "A prefix or suffix was defined for non-STR marker %s"
                        % marker)
                if PAT_SEQ_RAW.match(value) is None:
                    raise ValueError(
                        "Reference sequence '%s' of marker %s is invalid" %
                        (value, marker))
                library["nostr_reference"][marker] = value
                markers.add(marker)
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            elif section_low == "expected_allele_length":
                values = PAT_SPLIT.split(value)
                try:
                    min_length = int(values[0])
                except:
                    raise ValueError(
                        "Minimum expected allele length '%s' of marker %s "
                        "is not a valid integer" % (values[0], marker))
                if len(values) > 1:
                    try:
                        max_length = int(values[1])
                    except:
                        raise ValueError(
                            "Maximum expected allele length '%s' of marker %s "
                            "is not a valid integer" % (values[1], marker))
                else:
                    max_length = sys.maxint
                library["expected_length"][marker] = (min_length, max_length)
                markers.add(marker)
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    # Sanity check: prohibit prefix/suffix for aliases of non-STRs.
    for alias in library["aliases"]:
        if library["aliases"][alias]["marker"] in library["nostr_reference"] \
                and (alias in library["prefix"] or alias in library["suffix"]):
            raise ValueError(
                "A prefix or suffix was defined for alias %s of non-STR "
                "marker %s" % (alias, library["aliases"][alias]["marker"]))
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    # Sanity check: end position of marker should reflect ref length.
    for marker in library["genome_position"]:
        if marker not in library["nostr_reference"]:
            continue
        pos = library["genome_position"][marker]
        reflength = len(library["nostr_reference"][marker])
        length = 0
        for i in range(2, len(pos), 2):
            length += pos[i] - pos[i-1] + 1
        if reflength < length or (len(pos) % 2 and reflength != length):
            raise ValueError(
                "Length of reference sequence of marker %s is %i bases, but "
                "genome positions add up to %i bases" %
                (marker, reflength, length))

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    # Compile regular expressions.
    # NOTE: The libconvert tool expects "(seq){num,num}" blocks ONLY!
    for marker in markers:
        parts = []
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        blocksm = []
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        if marker in library["prefix"]:
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            parts += ("(%s){0,1}" % x for x in library["prefix"][marker])
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        if marker in library["aliases"]:
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            blocksm.append((library["aliases"][marker]["sequence"], 0, 1))
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        elif marker in library["regex"]:
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            blocksm += [(block[0], int(block[1]), int(block[2])) for block in
                        PAT_STR_DEF_BLOCK.findall(library["regex"][marker])]
        parts += ["(%s){%s,%s}" % x for x in blocksm]
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        if marker in library["suffix"]:
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            parts += ("(%s){0,1}" % x for x in library["suffix"][marker])
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        if parts:
            library["regex"][marker] = re.compile(
                "".join(["^"] + parts + ["$"]))
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        if blocksm:
            library["blocks_middle"][marker] = blocksm
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    return library
#parse_library_ini
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def load_profiles(profilefile, library=None):
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    column_names = profilefile.readline().rstrip("\r\n").split("\t")
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    (colid_marker, colid_allele, colid_sequence, colid_fmean, colid_rmean,
     colid_tool) = get_column_ids(column_names, "marker", "allele", "sequence",
        "fmean", "rmean", "tool")
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    profiles = {}
    for line in profilefile:
        line = line.rstrip("\r\n").split("\t")
        if line == [""]:
            continue
        marker = line[colid_marker]
        if marker not in profiles:
            profiles[marker] = {
                "m": set(),  # To be replaced by its length below.
                "n": set(),  # To be replaced by its length below.
                "seqs": [],
                "forward": {},  # To be replaced by a list below.
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                "reverse": {},  # To be replaced by a list below.
                "tool": {}  # To be replaced by a list below.
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                }
        allele = ensure_sequence_format(line[colid_allele], "raw",
            library=library, marker=marker)
        sequence = ensure_sequence_format(line[colid_sequence], "raw",
            library=library, marker=marker)
        if (allele, sequence) in profiles[marker]["forward"]:
            raise ValueError(
                "Invalid background noise profiles file: encountered "
                "multiple values for marker '%s' allele '%s' sequence '%s'" %
                (marker, allele, sequence))
        profiles[marker]["forward"][allele,sequence] = float(line[colid_fmean])
        profiles[marker]["reverse"][allele,sequence] = float(line[colid_rmean])
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        profiles[marker]["tool"][allele, sequence] = line[colid_tool]
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        profiles[marker]["m"].update((allele, sequence))
        profiles[marker]["n"].add(allele)

    # Check completeness and reorder true alleles.
    for marker in profiles:
        profiles[marker]["seqs"] = list(profiles[marker]["n"]) + \
            list(profiles[marker]["m"]-profiles[marker]["n"])
        profiles[marker]["n"] = len(profiles[marker]["n"])
        profiles[marker]["m"] = len(profiles[marker]["m"])
        newprofiles = {"forward": [], "reverse": []}
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        tools = []
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        for i in range(profiles[marker]["n"]):
            allele = profiles[marker]["seqs"][i]
            for direction in newprofiles:
                newprofiles[direction].append([0] * profiles[marker]["m"])
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            tools.append([""] * profiles[marker]["m"])
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            for j in range(profiles[marker]["m"]):
                sequence = profiles[marker]["seqs"][j]
                if (allele, sequence) in profiles[marker]["forward"]:
                    for direction in newprofiles:
                        newprofiles[direction][i][j] = \
                            profiles[marker][direction][allele, sequence]
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                    tools[i][j] = profiles[marker]["tool"][allele, sequence]
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        profiles[marker]["forward"] = newprofiles["forward"]
        profiles[marker]["reverse"] = newprofiles["reverse"]
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        profiles[marker]["tool"] = tools
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    return profiles
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#load_profiles
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def pattern_longest_match(pattern, subject):
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    """Return the longest match of the pattern in the subject string."""
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    # FIXME, this function tries only one match at each position in the
    # sequence, which is not neccessarily the longest match at that
    # position. For now, we'll search the reverse sequence as well.
    # Re-implement this without regular expressions to test all options.
    reverse = False
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    match = None
    pos = 0
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    pat = re.compile("".join("(%s){%i,%i}" % x for x in pattern))
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    while pos < len(subject):
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        m = pat.search(subject, pos)
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        if m is None:
            break
        if match is None or m.end()-m.start() > match.end()-match.start():
            match = m
        pos = m.start() + 1
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    # Try to find a longer match from the other end.
    if match is not None:
        subject = reverse_complement(subject)
        pos = 0
        pat = re.compile("".join(
            "(%s){%i,%i}" % (reverse_complement(x[0]), x[1], x[2])
            for x in reversed(pattern)))
        while pos < len(subject):
            m = pat.search(subject, pos)
            if m is None:
                break
            if m.end()-m.start() > match.end()-match.start():
                match = m
                reverse = True
            pos = m.start() + 1

    # Extract the blocks from the match.
    match = [] if match is None or not match.group() else reduce(
        lambda x, i: (
            x[0] + [match.group(i)]*((match.end(i)-x[1])/len(match.group(i))),
            match.end(i)) if match.group(i) else x,
        range(1, match.lastindex+1), ([], match.start()))[0]

    # Return the match in the same sequence orientation as the input.
    return map(reverse_complement, reversed(match)) if reverse else match
#pattern_longest_match


def pattern_longest_match_veryslow(pattern, subject):
    """Return the longest match of the pattern in the subject string."""
    longest = 0
    the_match = []
    # Generate all possible matching sequences for this pattern.
    #print("Finding match of pattern %r to sequence %s" % (pattern, subject))
    for matching_blocks in itertools.product(*(
            [[block[0]]*i for i in range(block[1], block[2]+1)]
            for block in pattern)):
        matching = itertools.chain.from_iterable(matching_blocks)
        matching_seq = "".join(matching)
        matching_len = len(matching_seq)
        if matching_len <= longest:
            continue
        if matching_seq in subject:
            longest = matching_len
            the_match = matching
    #print("Found match covering %i/%i bases" % (longest, len(subject)))
    return the_match
#pattern_longest_match_veryslow
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def detect_sequence_format(seq):
    """Return format of seq.  One of 'raw', 'tssv', or 'allelename'."""
    if not seq:
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        raise ValueError("Empty sequence")
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    if seq in SEQ_SPECIAL_VALUES:
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        # Special case.
        return False
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    if PAT_SEQ_RAW.match(seq):
        return 'raw'
    if PAT_SEQ_TSSV.match(seq):
        return 'tssv'
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    if PAT_SEQ_ALLELENAME_STR.match(seq) or PAT_SEQ_ALLELENAME_MT.match(seq) \
            or PAT_SEQ_ALLELENAME_SNP.match(seq):
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        return 'allelename'
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    raise ValueError("Unrecognised sequence format")
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#detect_sequence_format


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def convert_sequence_tssv_raw(seq):
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    return "".join(block[0] * int(block[1])
                   for block in PAT_TSSV_BLOCK.findall(seq))
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#convert_sequence_tssv_raw


def convert_sequence_raw_tssv(seq, library, marker, return_alias=False):
    # Try to match this marker's pattern, or any of its aliases.
    match = None
    if "aliases" in library:
        for alias in library["aliases"]:
            if (library["aliases"][alias]["marker"] == marker and
                    alias in library["regex"]):
                match = library["regex"][alias].match(seq)
                if match is not None:
                    marker = alias
                    break
    if match is None and marker in library["regex"]:
        match = library["regex"][marker].match(seq)
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    if match is not None:
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        parts = ((match.group(i), match.end(i)) for i in range(1, 1 if
            match.lastindex is None else match.lastindex+1) if match.group(i))
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    # Use heuristics if the sequence does not match the pattern.
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    else:
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        # Find explictily provided prefix and/or suffix if present.
        pre_suf = ["", ""]
        if "prefix" in library and marker in library["prefix"]:
            for prefix in library["prefix"][marker]:
                if seq.startswith(prefix):
                    pre_suf[0] = prefix
                    seq = seq[len(prefix):]
                    break
        if "suffix" in library and marker in library["suffix"]:
            for suffix in library["suffix"][marker]:
                if seq.endswith(suffix):
                    pre_suf[1] = suffix
                    seq = seq[:-len(suffix)]
                    break

        # Find longest match of middle pattern.
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        middle = [(seq, len(pre_suf[0])+len(seq))] if seq else []
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        if middle and marker in library["blocks_middle"]:
            match = pattern_longest_match(library["blocks_middle"][marker],seq)
            matched = "".join(match)
            if matched:
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                # If this allele does not match the prefix of this
                # marker, but the canonical prefix of the marker ends
                # with the same sequence as the start of our match, we
                # move that portion of the match into the prefix.
                # Then, we do the same thing with the suffix.
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                middle = match
                match_start = seq.index(matched)
                match_end = match_start + len(matched)
                start = match_start
                end = match_end
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                modified = False
                if (not pre_suf[0] and "prefix" in library
                        and marker in library["prefix"]):
                    ref = library["prefix"][marker][0]
                    i = min(len(ref), len(matched))
                    while i > 0:
                        if ref.endswith(matched[:i]):
                            start += i
                            matched = matched[i:]
                            modified = True
                            break
                        i -= 1
                if (not pre_suf[1] and "suffix" in library
                        and marker in library["suffix"]):
                    ref = library["suffix"][marker][0]
                    i = min(len(ref), len(matched))
                    while i > 0:
                        if ref.startswith(matched[-i:]):
                            end -= i
                            matched = matched[:-i]
                            modified = True
                            break
                        i -= 1
                if modified:
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                    from_start = start - match_start
                    from_end = match_end - end
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                    while from_start:
                        if from_start < len(middle[0]):
                            middle[0] = middle[0][from_start:]
                            break
                        else:
                            from_start -= len(middle[0])
                            middle = middle[1:]
                    while from_end:
                        if from_end < len(middle[-1]):
                            middle[-1] = middle[-1][:-from_end]
                            break
                        else:
                            from_end -= len(middle[-1])
                            middle = middle[:-1]
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                if middle:
                    middle = reduce(
                        lambda x, y: (x[:-1] if x[-1][0] == y else x) +
                            [(y, x[-1][1]+len(y))], middle[1:],
                            [(middle[0],
                              start+len(middle[0])+len(pre_suf[0]))])
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                pre_suf[0] += seq[:start]
                pre_suf[1] = seq[end:] + pre_suf[1]
                seq = matched
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        # Now construct parts.
        parts = []
        if pre_suf[0]:
            parts.append((pre_suf[0], len(pre_suf[0])))
        parts += middle
        if pre_suf[1]:
            parts.append((pre_suf[1], sum(map(len,pre_suf))+len(seq)))

    seq = reduce(
        lambda a, b: (a[0] + "%s(%i)" % (b[0], (b[1]-a[1])/len(b[0])), b[1]),
        reduce(
            lambda x, y: x[:-1] + [y] if x[-1][0] == y[0] else x + [y],
            parts,
            [("", 0)]))[0]
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    return (seq, marker) if return_alias else seq
#convert_sequence_raw_tssv


def convert_sequence_allelename_tssv(seq, library, marker):
    # Check whether there is an alias for this sequence.
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    alias_of = None
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    if "aliases" in library:
        for alias in library["aliases"]:
            if library["aliases"][alias]["marker"] == marker and (
                    seq == library["aliases"][alias]["name"] or
                    seq.startswith(library["aliases"][alias]["name"] + "_")):
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                alias_of = marker
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                marker = alias
                seq = "".join([
                    "0_",
                    library["aliases"][alias]["sequence"] + "[1]",
                    seq[len(library["aliases"][alias]["name"]):]])
                break

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    nameformat = None
    if PAT_SEQ_ALLELENAME_MT.match(seq) is not None:
        nameformat = "MtDNA"
    elif PAT_SEQ_ALLELENAME_SNP.match(seq) is not None:
        nameformat = "SNP"
    if nameformat is not None:
        # MtDNA and SNP markers.
        try:
            reference = library["nostr_reference"][marker]
        except KeyError:
            raise ValueError(
                "%s allele '%s' found for marker %s, but "
                "no reference sequence was found in the library" %
                (nameformat, seq, marker))
        if seq == "REF":
            return reference + "(1)"
        return mutate_sequence(reference, seq.split(),
            library["genome_position"].get(marker,
                ("M" if nameformat == "MtDNA" else "", 1))) + "(1)"

    # Note: aliases of mtDNA and SNP markers end up here as well.
    # It should NOT look like an alias now, however.
    match = PAT_SEQ_ALLELENAME_STR.match(seq)
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    if match is None or match.group(1) is not None:
        raise ValueError("Invalid allele name '%s' encountered!" % seq)

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    allele = seq.split("_")

    # Get and mutate prefix and suffix.
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    prefix = ""
    suffix = ""
    if "prefix" in library:
        if marker in library["prefix"]:
            prefix = library["prefix"][marker][0]
        elif alias_of is not None and alias_of in library["prefix"]:
            prefix = library["prefix"][alias_of][0]
    if "suffix" in library:
        if marker in library["suffix"]:
            suffix = library["suffix"][marker][0]
        elif alias_of is not None and alias_of in library["suffix"]:
            suffix = library["suffix"][alias_of][0]
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    variants = [[], []]
    for variant in allele[2:]:
        if variant[0] == "-":
            if not prefix:
                raise ValueError("Encountered prefix variant '%s', but marker "
                                 "'%s' has no prefix!" % (variant, marker))
            variants[0].append(variant)
        elif variant[0] == "+":
            if not suffix:
                raise ValueError("Encountered suffix variant '%s', but marker "
                                 "'%s' has no suffix!" % (variant, marker))
            variants[1].append(variant)
        else:
            raise ValueError("Unrecognised variant '%s'" % variant)
    if variants[0]:
        prefix = mutate_sequence(prefix, variants[0])
    if variants[1]:
        suffix = mutate_sequence(suffix, variants[1])

    blocks = []
    if prefix:
        blocks.append((prefix, 1))
    for block in PAT_ALLELENAME_BLOCK.findall(allele[1]):
        blocks.append((block[0], int(block[1])))
    if suffix:
        blocks.append((suffix, 1))
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    return "".join("%s(%i)" % block for block in blocks)
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#convert_sequence_allelename_tssv


def convert_sequence_raw_allelename(seq, library, marker):
    # We actually convert raw->allelename via TSSV format.
    seq, alias = convert_sequence_raw_tssv(seq, library, marker, True)
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    blocks = PAT_TSSV_BLOCK.findall(seq)

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    if "nostr_reference" in library and marker in library["nostr_reference"]:
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        # Handle non-STR markers here.
        if alias != marker:
            return library["aliases"][alias]["name"]
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        if not blocks:
            # Oh dear, empty sequence... Primer dimer?
            blocks = (("",),)
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        if library["nostr_reference"][marker] == blocks[0][0]:
            return "REF"
        return " ".join(
            call_variants(library["nostr_reference"][marker], blocks[0][0],
                library["genome_position"].get(marker, "suffix")))

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    # Find prefix and suffix.
    prefix = suffix = this_prefix = this_suffix = ""
    remaining_blocks = len(blocks)
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    if "prefix" in library:
        if alias in library["prefix"]:
            prefix = library["prefix"][alias][0]
        elif marker in library["prefix"]:
            prefix = library["prefix"][marker][0]
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        if prefix and remaining_blocks > 0 and blocks[0][1] == "1":
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            remaining_blocks -= 1
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    if "suffix" in library:
        if alias in library["suffix"]:
            suffix = library["suffix"][alias][0]
        elif marker in library["suffix"]:
            suffix = library["suffix"][marker][0]
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        if suffix and remaining_blocks > 0 and blocks[-1][1] == "1":
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            remaining_blocks -= 1
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    if remaining_blocks > 0 and prefix and blocks[0][1] == "1":
        this_prefix = blocks[0][0]
        blocks = blocks[1:]
    if remaining_blocks > 0 and suffix and blocks[-1][1] == "1":
        this_suffix = blocks[-1][0]
        blocks = blocks[:-1]
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    # Generate prefix/suffix variants.
    length = 0
    variants = []
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    if prefix != this_prefix:
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        variants += call_variants(prefix, this_prefix, "prefix")
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        length += len(this_prefix) - len(prefix)
    if suffix != this_suffix:
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        variants += call_variants(suffix, this_suffix, "suffix")
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        length += len(this_suffix) - len(suffix)
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    # We are ready to return the allele name of aliases.
    if alias != marker:
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        return "_".join([library["aliases"][alias]["name"]] + variants)
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    # Compute CE allele number for the other alleles.
    # TODO: perhaps produce a more intelligent name if there is exactly
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    #       1 alias with the same length, or only 1 alias sequence is
    #       contained somewhere within the allele.
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    blocknames = []
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    blocksize = library.get("block_length", {}).get(marker, 4)
    length -= library.get("length_adjust", {}).get(marker, 0)
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    for block in blocks:
        blocknames.append("%s[%s]" % (block[0], block[1]))
        length += len(block[0]) * int(block[1])

    allelename = "CE" + str(length / blocksize)
    if length % blocksize:
        allelename += "." + str(length % blocksize)
    return "_".join([allelename, "".join(blocknames)] + variants)
#convert_sequence_raw_allelename


def ensure_sequence_format(seq, to_format, from_format=None, library=None,
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                           marker=None):
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    """Convert seq to 'raw', 'tssv', or 'allelename' format."""
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    if seq in SEQ_SPECIAL_VALUES:
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        # Special case.
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        return seq
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    known_formats = ("raw", "tssv", "allelename")
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    if to_format not in known_formats:
        raise ValueError("Unknown format '%s', choose from %s" %
                         (to_format, known_formats))
    if from_format is None:
        from_format = detect_sequence_format(seq) if seq else "raw"
    elif from_format not in known_formats:
        raise ValueError("Unknown format '%s', choose from %s" %
                         (from_format, known_formats))

    # No conversion needed?
    if to_format == from_format:
        return seq

    # From TSSV to raw sequence is easy.
    # We'll need a library and marker name for anything else.
    if (library is None or marker is None) and (from_format != "tssv" or
            to_format != "raw"):
        raise ValueError("Sequence needs to be converted from %s to %s, this "
                          "conversion requires a library file" %
                          (from_format, to_format))

    # Perform conversions.
    if from_format == "allelename":
        seq = convert_sequence_allelename_tssv(seq, library, marker)
    if to_format == "tssv":
        if from_format == "raw":
            return convert_sequence_raw_tssv(seq, library, marker)
        return seq
    if from_format != "raw":
        seq = convert_sequence_tssv_raw(seq)
    if to_format == "raw":
        return seq
    return convert_sequence_raw_allelename(seq, library, marker)
#ensure_sequence_format


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def reverse_complement(sequence):
    """Return the reverse complement of the given DNA sequence."""
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    return "".join(COMPL[x] if x in COMPL else x for x in reversed(sequence))
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#reverse_complement


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def nnls(A, C, B=None, max_iter=200, min_change=0.0001, debug=False):
    """
    Solve for B in A * B = C in the least squares sense, s.t. B >= 0.

    Hint: call nnls(B.T, C.T).T to solve for A.

    Algorithm has converged if the sum of squared error has decreased
    by less than a factor of min_change in one iteration.  If debug is
    True, print the sum of squared error to sys.stdout after each
    iteration.

    This code was partially adopted from nimfa.methods.factorization.bd.
    """
    import numpy as np
    if B is None:
        B = np.matrix(np.zeros([A.shape[1], C.shape[1]]))
    E = A.T * A
    F = A.T * C
    prev_score = cur_score = sys.float_info.max
    for i in range(max_iter):
        for n in range(B.shape[0]):
            nn = list(range(n)) + list(range(n + 1, B.shape[0]))
            tmp = (F[n, :] - E[n, nn] * B[nn, :])