Commit 288e605c by Laros

### Got rid of the class.

parent 35e0fc3d
 ... ... @@ -7,7 +7,7 @@ Copyright (c) 2013-2016 Jeroen F.J. Laros Licensed under the MIT license, see the LICENSE file. """ from .barcode import BarCode from .barcode import filter_distance, all_barcodes, filter_stretches __version_info__ = ('0', '6', '0') ... ...
 import Levenshtein class BarCode(object): """ Design and test NGS barcodes. """ _nucleotides = ['A', 'C', 'G', 'T'] _nucleotides = ['A', 'C', 'G', 'T'] def __init__(self, distance=Levenshtein.distance): """ Initialise the class. :arg function distance: Distance function. """ self.distance = distance def _all_words(self, bucket, word, length, result): def _all_words(bucket, word, length, result): """ Generate all possible words of a certain length over a specified alphabet. ... ... @@ -27,11 +16,12 @@ class BarCode(object): """ if length: for i in bucket: self._all_words(bucket, word + i, length - 1, result) _all_words(bucket, word + i, length - 1, result) else: result.append(word) def _filter_stretch(self, barcode, stretches): def _filter_stretch(barcode, stretches): """ Test whether {barcode} contains none of the stretches in {stretches}. ... ... @@ -46,7 +36,8 @@ class BarCode(object): return True def _filter_distance(self, barcodes, candidate, min_dist): def _filter_distance(barcodes, candidate, min_dist, distance): """ Test whether {candidate} can be added to {barcodes} based on the minimum distance between {candidate} and all barcodes in {barcodes}. ... ... @@ -54,16 +45,18 @@ class BarCode(object): :arg list barcodes: List of barcodes. :arg str candidate: Candidate barcode. :arg int min_dist: Minimum distance between the barcodes. :arg function distance: Distance function. :returns bool: True if the barcode is clean, False otherwise. """ for i in barcodes: if self.distance(i, candidate) < min_dist: if distance(i, candidate) < min_dist: return False return True def all_barcodes(self, length): def all_barcodes(length): """ Generate all possible barcodes of a certain length. ... ... @@ -73,11 +66,12 @@ class BarCode(object): """ result = [] self._all_words(self._nucleotides, '', length, result) _all_words(_nucleotides, '', length, result) return result def filter_stretches(self, barcodes, max_stretch): def filter_stretches(barcodes, max_stretch): """ Filter a list of barcodes for mononucleotide stretches. ... ... @@ -86,21 +80,23 @@ class BarCode(object): :returns list: List of barcodes filtered for mononucleotide stretches. """ stretches = map(lambda x: (max_stretch + 1) * x, self._nucleotides) stretches = map(lambda x: (max_stretch + 1) * x, _nucleotides) result = [] for i in barcodes: if self._filter_stretch(i, stretches): if _filter_stretch(i, stretches): result.append(i) return result def filter_distance(self, barcodes, min_dist): def filter_distance(barcodes, min_dist, distance=Levenshtein.distance): """ Filter a list of barcodes for distance to other barcodes. :arg list barcodes: List of barcodes. :arg int min_dist: Minimum distance between the barcodes. :arg function distance: Distance function. :returns list: List of barcodes filtered for distance to other barcodes. ... ... @@ -108,7 +104,7 @@ class BarCode(object): result = [] for i in barcodes: if self._filter_distance(result, i, min_dist): if _filter_distance(result, i, min_dist, distance): result.append(i) return result
 ... ... @@ -5,8 +5,8 @@ import sys import Levenshtein from . import doc_split, version, usage from .barcode import BarCode from . import doc_split, usage, version from .barcode import all_barcodes, filter_distance, filter_stretches def make_barcodes(length, max_stretch, min_dist, distance): ... ... @@ -19,10 +19,8 @@ def make_barcodes(length, max_stretch, min_dist, distance): :arg int min_dist: Minimum distance between the barcodes. :arg function distance: Distance function. """ bc = BarCode(distance) return bc.filter_distance( bc.filter_stretches(bc.all_barcodes(length), max_stretch), min_dist) return filter_distance( filter_stretches(all_barcodes(length), max_stretch), min_dist) def test_barcodes(barcodes, min_dist, distance, handle): ... ... @@ -32,12 +30,11 @@ def test_barcodes(barcodes, min_dist, distance, handle): :arg list barcodes: List of barcodes. :arg int min_dist: Minimum distance between the barcodes. :arg function distance: Distance function. :arg steam handle: Open readable handle to a file. :returns int: The number of barcodes that violate the distance constraint. """ bc = BarCode(distance) good_subset = bc.filter_distance(barcodes, min_dist) good_subset = filter_distance(barcodes, min_dist) if handle: handle.write('\n'.join(good_subset)) ... ...
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