version 1.0 # Copyright (c) 2017 Leiden University Medical Center # # Permission is hereby granted, free of charge, to any person obtaining a copy # of this software and associated documentation files (the "Software"), to deal # in the Software without restriction, including without limitation the rights # to use, copy, modify, merge, publish, distribute, sublicense, and/or sell # copies of the Software, and to permit persons to whom the Software is # furnished to do so, subject to the following conditions: # # The above copyright notice and this permission notice shall be included in # all copies or substantial portions of the Software. # # THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR # IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, # FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE # AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER # LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, # OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE # SOFTWARE. task Unicycler { input { String out String? preCommand File? short1 File? short2 File? unpaired File? long Int? verbosity Int? minFastaLength Int? keep Boolean? vcf String? mode Float? minBridgeQual Int? linearSeqs File? spadesPath Boolean? noCorrect Float? minKmerFrac Float? maxKmerFrac Int? kmerCount Float? depthFilter Boolean? noMiniasm File? raconPath File? existingLongReadAssembly Boolean? noRotate File? startGenes Float? startGeneId Float? startGeneCov String? makeblastdbPath File? tblastnPath Boolean? noPilon File? bowtie2Path File? bowtie2buildPath File? samtoolsPath File? pilonPath File? javaPath Int? minPolishSize File? bcftoolsPath Int? minComponentSize Int? minDeadEndSize File? contamination String? scores String? lowScore Int threads = 1 String memory = "4GiB" } command { set -e -o pipefail mkdir -p ~{out} ~{preCommand} unicycler \ ~{"--short1 " + short1} \ ~{"--short2 " + short2} \ ~{"--unpaired " + unpaired} \ ~{"--long " + long} \ --out ~{out} \ ~{"--min_fasta_length " + minFastaLength} \ ~{"--keep " + keep } \ ~{true="--vcf" false="" vcf } \ ~{"--threads " + threads } \ ~{"--mode " + mode } \ ~{"--min_bridge_qual " + minBridgeQual } \ ~{"--linear_seqs " + linearSeqs } \ ~{"--spades_path " + spadesPath } \ ~{true="--no_correct" false="" noCorrect } \ ~{"--min_kmer_frac " + minKmerFrac } \ ~{"--max_kmer_frac " + maxKmerFrac } \ ~{"--kmer_count " + kmerCount } \ ~{"--depth_filter " + depthFilter } \ ~{true="--no_miniasm" false="" noMiniasm } \ ~{"--racon_path " + raconPath } \ ~{"--existing_long_read_assembly " + existingLongReadAssembly } \ ~{true="--no_rotate" false="" noRotate } \ ~{"--start_genes " + startGenes } \ ~{"--start_gene_id " + startGeneId } \ ~{"--start_gene_cov " + startGeneCov } \ ~{"--makeblastdb_path " + makeblastdbPath } \ ~{"--tblastn_path " + tblastnPath } \ ~{true="--no_pilon" false="" noPilon } \ ~{"--bowtie2_path " + bowtie2Path } \ ~{"--bowtie2_build_path " + bowtie2buildPath } \ ~{"--samtools_path " + samtoolsPath } \ ~{"--pilon_path " + pilonPath } \ ~{"--java_path " + javaPath } \ ~{"--min_polish_size " + minPolishSize } \ ~{"--bcftools_path " + bcftoolsPath } \ ~{"--min_component_size " + minComponentSize } \ ~{"--min_dead_end_size " + minDeadEndSize } \ ~{"--contamination " + contamination } \ ~{"--scores " + scores } \ ~{"--low_score " + lowScore } } output { File assemblyFasta = out + "/assembly.fasta" File assemblyGfa = out + "/assembly.gfa" File log = out + "/unicycler.log" } runtime { cpu: threads memory: memory } }