version 1.0 # Copyright (c) 2017 Leiden University Medical Center # # Permission is hereby granted, free of charge, to any person obtaining a copy # of this software and associated documentation files (the "Software"), to deal # in the Software without restriction, including without limitation the rights # to use, copy, modify, merge, publish, distribute, sublicense, and/or sell # copies of the Software, and to permit persons to whom the Software is # furnished to do so, subject to the following conditions: # # The above copyright notice and this permission notice shall be included in # all copies or substantial portions of the Software. # # THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR # IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, # FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE # AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER # LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, # OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE # SOFTWARE. task HTSeqCount { input { Array[File]+ inputBams File gtfFile String outputTable = "output.tsv" String order = "pos" String stranded = "no" String? featureType String? idattr Array[String] additionalAttributes = [] Int threads = 1 String memory = "8G" Int timeMinutes = 10 + ceil(size(inputBams, "G") * 60) String dockerImage = "quay.io/biocontainers/htseq:0.12.4--py37hb3f55d8_0" } command { set -e mkdir -p "$(dirname ~{outputTable})" htseq-count \ --nprocesses ~{threads} \ -r ~{order} \ -s ~{stranded} \ ~{"--type " + featureType} \ ~{"--idattr " + idattr} \ ~{true="--additional-attr " false="" length(additionalAttributes) > 0 }~{sep=" --additional-attr " additionalAttributes} \ ~{sep=" " inputBams} \ ~{gtfFile} \ -c ~{outputTable} } output { File counts = outputTable } runtime { cpu: threads time_minutes: timeMinutes memory: memory docker: dockerImage } parameter_meta { inputBams: {description: "The input BAM files.", category: "required"} gtfFile: {description: "A GTF/GFF file containing the features of interest.", category: "required"} outputTable: {description: "The path to which the output table should be written.", category: "common"} format: {description: "Equivalent to the -f option of htseq-count.", category: "advanced"} order: {description: "Equivalent to the -r option of htseq-count.", category: "advanced"} stranded: {description: "Equivalent to the -s option of htseq-count.", category: "common"} featureType: {description: "Equivalent to the --type option of htseq-count.", category: "advanced"} idattr: {description: "Equivalent to the --idattr option of htseq-count.", category: "advanced"} additionalAttributes: {description: "Equivalent to the --additional-attr option of htseq-count.", category: "advanced"} memory: {description: "The amount of memory the job requires in GB.", category: "advanced"} timeMinutes: {description: "The maximum amount of time the job will run in minutes.", category: "advanced"} dockerImage: {description: "The docker image used for this task. Changing this may result in errors which the developers may choose not to address.", category: "advanced"} } }