diff --git a/CHANGELOG.md b/CHANGELOG.md index 22f4182626273068c3dc6f8faa1b3722574762ef..424dc76444073fce38836e0a6264c7ea7a5e6a9d 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -10,6 +10,7 @@ that users understand how the changes affect the new version. version 5.0.0-dev --------------------------- ++ Add new wdl file prepareShiny.wdl for creating input files for shiny app. + mergePacBio: Rename `mergedReport` to `outputPathMergedReport`. + Lima: Fix copy commands. + Fixed the `size` call in the default for gffread's timeMinutes, to retrieve diff --git a/prepareShiny.wdl b/prepareShiny.wdl new file mode 100644 index 0000000000000000000000000000000000000000..d669e2d1791fc8ea7462198c4493cfe5c3c342f5 --- /dev/null +++ b/prepareShiny.wdl @@ -0,0 +1,106 @@ +version 1.0 + +# Copyright (c) 2017 Sequencing Analysis Support Core - Leiden University Medical Center +# +# Permission is hereby granted, free of charge, to any person obtaining a copy +# of this software and associated documentation files (the "Software"), to deal +# in the Software without restriction, including without limitation the rights +# to use, copy, modify, merge, publish, distribute, sublicense, and/or sell +# copies of the Software, and to permit persons to whom the Software is +# furnished to do so, subject to the following conditions: + +# The above copyright notice and this permission notice shall be included in all +# copies or substantial portions of the Software. + +# THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR +# IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, +# FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE +# AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER +# LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, +# OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE +# SOFTWARE. + +task CreateDesignMatrix { + input { + File countTable + String shinyDir = "." + + String memory = "5G" + Int timeMinutes = 30 + String dockerImage = "quay.io/biocontainers/predex:0.9.2--pyh3252c3a_0" + } + + command { + set -e + mkdir -p ~{shinyDir} + predex design \ + --input ~{countTable} \ + --output ~{shinyDir} + } + + output { + File dgeDesign = shinyDir + "/design_matrix.tsv" + } + + runtime { + memory: memory + time_minutes: timeMinutes + docker: dockerImage + } + + parameter_meta { + # inputs + countTable: {description: "The created count table from HTseq.", category: "required"} + shinyDir: {description: "The directory to write the output to.", category: "required"} + memory: {description: "The amount of memory this job will use.", category: "advanced"} + timeMinutes: {description: "The maximum amount of time the job will run in minutes.", category: "advanced"} + dockerImage: {description: "The docker image used for this task. Changing this may result in errors which the developers may choose not to address.", category: "advanced"} + + # outputs + dgeDesign: {description: "Design matrix template to add sample information for DGE analysis."} + } +} + +task CreateAnnotation { + input { + File referenceFasta + File referenceGtfFile + String shinyDir = "." + + String memory = "5G" + Int timeMinutes = 30 + String dockerImage = "quay.io/biocontainers/predex:0.9.2--pyh3252c3a_0" + } + + command { + set -e + mkdir -p ~{shinyDir} + predex annotation \ + --fasta ~{referenceFasta} \ + --gtf ~{referenceGtfFile} \ + --output ~{shinyDir} + } + + output { + File dgeAnnotation = shinyDir + "/annotation.tsv" + } + + runtime { + memory: memory + time_minutes: timeMinutes + docker: dockerImage + } + + parameter_meta { + # inputs + referenceFasta: {description: "The reference Fasta file.", category: "required"} + referenceGtfFile: {description: "The reference GTF file.", category: "required"} + shinyDir: {description: "The directory to write the output to.", category: "required"} + memory: {description: "The amount of memory this job will use.", category: "advanced"} + timeMinutes: {description: "The maximum amount of time the job will run in minutes.", category: "advanced"} + dockerImage: {description: "The docker image used for this task. Changing this may result in errors which the developers may choose not to address.", category: "advanced"} + + # outputs + dgeAnnotation: {description: "Annotation file for DGE analysis."} + } +}