diff --git a/fgbio.wdl b/fgbio.wdl new file mode 100644 index 0000000000000000000000000000000000000000..d50906d33ef5cfd62ad004561039379c074d15ab --- /dev/null +++ b/fgbio.wdl @@ -0,0 +1,68 @@ +version 1.0 + +# Copyright (c) 2017 Leiden University Medical Center +# +# Permission is hereby granted, free of charge, to any person obtaining a copy +# of this software and associated documentation files (the "Software"), to deal +# in the Software without restriction, including without limitation the rights +# to use, copy, modify, merge, publish, distribute, sublicense, and/or sell +# copies of the Software, and to permit persons to whom the Software is +# furnished to do so, subject to the following conditions: +# +# The above copyright notice and this permission notice shall be included in +# all copies or substantial portions of the Software. +# +# THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR +# IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, +# FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE +# AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER +# LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, +# OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE +# SOFTWARE. + +task AnnotateBamWithUmis { + input { + File inputBam + File inputUmi + String outputPath + + String memory = "120G" + Int timeMinutes = 360 + String javaXmx="100G" + String dockerImage = "quay.io/biocontainers/fgbio:1.4.0--hdfd78af_0" + } + + command { + set -e + mkdir -p "$(dirname ~{outputPath})" + fgbio -Xmx~{javaXmx} \ + AnnotateBamWithUmis \ + -i ~{inputBam} \ + -f ~{inputUmi} \ + -o ~{outputPath} + } + + output { + File outputBam = outputPath + } + + runtime { + memory: memory + time_minutes: timeMinutes + docker: dockerImage + } + + parameter_meta { + # inputs + inputBam: {description: "The input BAM file.", category: "required"} + inputUmi: {description: "The input fastq file with UMIs.", category: "required"} + outputPath: {description: "Output directory path + output file.", category: "required"} + javaXmx: {description: "The maximum memory available to the program. Should be lower than `memory` to accommodate JVM overhead.", category: "advanced"} + memory: {description: "The amount of memory this job will use.", category: "advanced"} + timeMinutes: {description: "The maximum amount of time the job will run in minutes.", category: "advanced"} + dockerImage: {description: "The docker image used for this task. Changing this may result in errors which the developers may choose not to address.", category: "advanced"} + + # outputs + outputBam: {description: "UMI-annotated output BAM file."} + } +}